PMID- 38293066
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240131
DP  - 2024 Jan 16
TI  - Forced mouth opening induces post-traumatic hyperalgesia and associated 
      peripheral sensitization after temporomandibular joints injury in mice.
LID - 2024.01.16.575891 [pii]
LID - 10.1101/2024.01.16.575891 [doi]
AB  - Temporomandibular disorder (TMD) is the most prevalent painful condition in the 
      craniofacial area. The pathophysiology of TMD is not fully understood, and it is 
      necessary to understand pathophysiology underlying painful TMD conditions to 
      develop more effective treatment methods. Recent studies suggested that external 
      or intrinsic trauma to TMJ is associated with chronic TMD in patients. Here, we 
      investigated the effects of the TMJ trauma through forced-mouth opening (FMO) in 
      mice to determine pain behaviors and peripheral sensitization of trigeminal 
      nociceptors. FMO increased mechanical hyperalgesia assessed by von Frey test, 
      spontaneous pain-like behaviors assessed by mouse grimace scale, and anxiety-like 
      behaviors assessed by open-field test. In vivo GCaMP Ca (2+) imaging of intact 
      trigeminal ganglia (TG) showed increased spontaneous Ca (2+) activity and 
      mechanical hypersensitivity of TG neurons in the FMO compared to the sham group. 
      Ca (2+) responses evoked by cold, heat, and capsaicin stimuli were also 
      increased. FMO-induced hyperalgesia and neuronal hyperactivities were not sex 
      dependent. TG neurons sensitized following FMO were primarily small to 
      medium-sized nociceptive afferents. Consistently, most TMJ afferents in the TG 
      were small-sized peptidergic neurons expressing calcitonin gene-related peptides, 
      whereas nonpeptidergic TMJ afferents were relatively low. FMO-induced intraneural 
      inflammation in the surrounding tissues of the TMJ indicates potentially novel 
      mechanisms of peripheral sensitization following TMJ injury. These results 
      suggest that the TMJ injury leads to persistent post-traumatic hyperalgesia 
      associated with peripheral sensitization of trigeminal nociceptors.
FAU - Alshanqiti, Ishraq
AU  - Alshanqiti I
FAU - Son, Hyeonwi
AU  - Son H
FAU - Shannonhouse, John
AU  - Shannonhouse J
FAU - Hu, Jiaxin
AU  - Hu J
FAU - Kumari, Sinu
AU  - Kumari S
FAU - Parastooei, Ghazaal
AU  - Parastooei G
FAU - Wang, Sheng
AU  - Wang S
FAU - Ro, Jin Y
AU  - Ro JY
FAU - Kim, Yu Shin
AU  - Kim YS
FAU - Chung, Man-Kyo
AU  - Chung MK
LA  - eng
PT  - Preprint
DEP - 20240116
PL  - United States
TA  - bioRxiv
JT  - bioRxiv : the preprint server for biology
JID - 101680187
PMC - PMC10827102
EDAT- 2024/01/31 06:43
MHDA- 2024/01/31 06:44
PMCR- 2024/01/30
CRDT- 2024/01/31 04:19
PHST- 2024/01/31 06:44 [medline]
PHST- 2024/01/31 06:43 [pubmed]
PHST- 2024/01/31 04:19 [entrez]
PHST- 2024/01/30 00:00 [pmc-release]
AID - 2024.01.16.575891 [pii]
AID - 10.1101/2024.01.16.575891 [doi]
PST - epublish
SO  - bioRxiv [Preprint]. 2024 Jan 16:2024.01.16.575891. doi: 
      10.1101/2024.01.16.575891.