PMID- 38293551 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240201 IS - 2405-8440 (Print) IS - 2405-8440 (Electronic) IS - 2405-8440 (Linking) VI - 10 IP - 2 DP - 2024 Jan 30 TI - Triptolide attenuates cardiac remodeling by inhibiting pyroptosis and EndMT via modulating USP14/Keap1/Nrf2 pathway. PG - e24010 LID - 10.1016/j.heliyon.2024.e24010 [doi] LID - e24010 AB - BACKGROUND: Cardiac remodeling is a common pathological feature in many cardiac diseases, characterized by cardiac hypertrophy and fibrosis. Triptolide (TP) is a natural compound derived from Tripterygium wilfordii Hook F. However, the related mechanism of it in cardiac remodeling has not been fully understood. METHODS AND RESULTS: Transverse aortic constriction (TAC)-induced cardiac hypertrophic mouse model and angiotensin II (Ang II)-induced cardiomyocytes hypertrophic model were performed. Firstly, the results indicate that TP can improve cardiac function, decreased cardiomyocyte surface area and fibrosis area, as well as lowered the protein expressions of brain natriuretic peptide (BNP), beta-major histocompatibility complex (beta-MHC), type I and III collagen (Col I and III). Secondly, TP suppressed cardiac pyroptosis, and decreased the levels of Interleukin-1beta (IL-1beta), Interleukin-18 (IL-18) by Enzyme-linked immunosorbent assay (ELISA), and pyroptosis-associated proteins. Furthermore, TP enhanced the expressions of Nuclear factor erythroid 2-related factor 2 (Nrf2) and Heme oxygenase 1 (HO-1). Interestingly, when Nrf2 was silenced by siRNA, TP lost its properties of reducing pyroptosis and cardiac hypertrophy. In addition, in the Transforming Growth Factor beta1 (TGF-beta1)-induced primary human coronary artery endothelial cells (HCAEC) model, TP was found to inhibit the process of endothelial-to-mesenchymal transition (EndMT), characterized by the loss of endothelial-specific markers and the gain of mesenchymal markers. This was accompanied by a suppression of Slug, Snail, and Twist expression. Meanwhile, the inhibitory effect of TP on EndMT was weakened when Nrf2 was silenced by siRNA. Lastly, potential targets of TP were identified through network pharmacology analysis, and found that Ubiquitin-Specific Protease 14 (USP14) was one of them. Simultaneously, the data indicated that decrease the upregulation of USP14 and Kelch-like ECH-Associated Protein 1 (Keap1) caused by cardiac remodeling. However, Keap1 was decreased and Nrf2 was increased when USP14 was silenced. Furthermore, CoIP analysis showed that USP14 directly interacts with Keap1. CONCLUSION: TP can observably reduce pyroptosis and EndMT by targeting the USP14/Keap1/Nrf2 pathway, thereby significantly attenuating cardiac remodeling. CI - (c) 2024 The Authors. Published by Elsevier Ltd. FAU - Ba, Lina AU - Ba L AD - Department of Pharmacology, State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Medicine Research, Ministry of Education, College of Pharmacy, Harbin Medical University, Harbin, 150081, China. AD - Department of Pharmacology, Harbin Medical University-Daqing, Daqing, Heilongjiang, 163319, China. FAU - E, Mingyao AU - E M AD - Department of Pharmacology, Harbin Medical University-Daqing, Daqing, Heilongjiang, 163319, China. AD - Key Laboratory of Bio-Macromolecules of Chinese Medicine, Changchun University of Chinese Medicine, Changchun, 130117, China. FAU - Wang, Ruixuan AU - Wang R AD - Department of Pharmacology, Harbin Medical University-Daqing, Daqing, Heilongjiang, 163319, China. FAU - Wu, Nan AU - Wu N AD - Department of Pharmacology, Harbin Medical University-Daqing, Daqing, Heilongjiang, 163319, China. FAU - Wang, Rui AU - Wang R AD - Department of Pharmacology, Harbin Medical University-Daqing, Daqing, Heilongjiang, 163319, China. FAU - Liu, Renling AU - Liu R AD - Department of Pharmacology, Harbin Medical University-Daqing, Daqing, Heilongjiang, 163319, China. FAU - Feng, Xiang AU - Feng X AD - Department of Pharmacology, Harbin Medical University-Daqing, Daqing, Heilongjiang, 163319, China. FAU - Qi, Hanping AU - Qi H AD - Department of Pharmacology, Harbin Medical University-Daqing, Daqing, Heilongjiang, 163319, China. FAU - Sun, Hongli AU - Sun H AD - Department of Pharmacology, Harbin Medical University-Daqing, Daqing, Heilongjiang, 163319, China. FAU - Qiao, Guofen AU - Qiao G AD - Department of Pharmacology, State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Medicine Research, Ministry of Education, College of Pharmacy, Harbin Medical University, Harbin, 150081, China. LA - eng PT - Journal Article DEP - 20240103 PL - England TA - Heliyon JT - Heliyon JID - 101672560 PMC - PMC10825440 OTO - NOTNLM OT - Cardiac fibrosis OT - Cardiac hypertrophy OT - Cardiac remodeling OT - EndMT OT - Pyroptosis OT - Triptolide COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2024/01/31 06:42 MHDA- 2024/01/31 06:43 PMCR- 2024/01/03 CRDT- 2024/01/31 04:28 PHST- 2023/08/04 00:00 [received] PHST- 2023/12/21 00:00 [revised] PHST- 2024/01/02 00:00 [accepted] PHST- 2024/01/31 06:43 [medline] PHST- 2024/01/31 06:42 [pubmed] PHST- 2024/01/31 04:28 [entrez] PHST- 2024/01/03 00:00 [pmc-release] AID - S2405-8440(24)00041-0 [pii] AID - e24010 [pii] AID - 10.1016/j.heliyon.2024.e24010 [doi] PST - epublish SO - Heliyon. 2024 Jan 3;10(2):e24010. doi: 10.1016/j.heliyon.2024.e24010. eCollection 2024 Jan 30.