PMID- 38295722
OWN - NLM
STAT- MEDLINE
DCOM- 20240219
LR  - 20240219
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1234
DP  - 2024 Feb 15
TI  - Facial on-line enrichment of glycoproteins by capillary electrophoresis with 
      boronate-functionalized poly(glycidyl methacrylate) microparticles coated column.
PG  - 124013
LID - S1570-0232(24)00021-7 [pii]
LID - 10.1016/j.jchromb.2024.124013 [doi]
AB  - A facial and rapid method for glycoproteins enrichment by capillary 
      electrophoresis was developed. The 3-aminophenylboronic acid-functionalized 
      poly(glycidyl methacrylate) microparticles (PGMA@APBA) were attached to the 
      capillary inlet (length of  approximately 1.5 cm) by electrostatic self-assemble action to 
      prepare a partially coated capillary column. The process is simple and 
      reversible, allowing for easy renewal of the PGMA@APBA coating when its 
      enrichment efficiency decreases. By utilizing the coated column, glycoproteins 
      can be enriched within 2 min. The column exhibits a specific enrichment for 
      glycoproteins and can be consecutively used for approximately 60 runs. The 
      relative standard deviations (RSDs) of peak area of run-to-run (n = 5) and 
      batch-to-batch (n = 3) were 1.5 % and 1.0%, respectively. The method was 
      successfully applied to enrich glycoproteins from 1 x 10(12)-fold diluted real 
      egg white sample, indicating its practical applicability.
CI  - Copyright (c) 2024 Elsevier B.V. All rights reserved.
FAU - Zhang, Jian
AU  - Zhang J
AD  - School of Pharmacy, Xi' an Medical University, Xi'an 710021, China; Institute of 
      Medicine, Xi' an Medical University, Xi'an 710021, China.
FAU - Miao, Yanqing
AU  - Miao Y
AD  - School of Pharmacy, Xi' an Medical University, Xi'an 710021, China; Institute of 
      Medicine, Xi' an Medical University, Xi'an 710021, China.
FAU - Jing, Hui
AU  - Jing H
AD  - School of Pharmacy, Xi' an Medical University, Xi'an 710021, China; Institute of 
      Medicine, Xi' an Medical University, Xi'an 710021, China.
FAU - Wu, Jingwen
AU  - Wu J
AD  - School of Pharmacy, Xi' an Medical University, Xi'an 710021, China.
FAU - Liu, Chunye
AU  - Liu C
AD  - School of Pharmacy, Xi' an Medical University, Xi'an 710021, China; Institute of 
      Medicine, Xi' an Medical University, Xi'an 710021, China. Electronic address: 
      liuchunye@xiyi.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20240126
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 25067-05-4 (polyglycidyl methacrylate)
RN  - 0 (Polymethacrylic Acids)
RN  - R8WN29J8VF (glycidyl methacrylate)
RN  - 0 (Glycoproteins)
RN  - 0 (Epoxy Compounds)
RN  - 0 (Methacrylates)
SB  - IM
MH  - *Electrophoresis, Capillary
MH  - *Polymethacrylic Acids
MH  - Glycoproteins
MH  - *Epoxy Compounds
MH  - *Methacrylates
OTO - NOTNLM
OT  - Boronate
OT  - Capillary electrophoresis
OT  - Glycoprotein
OT  - On-line enrichment
OT  - Poly(glycidyl methacrylate) microparticles
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2024/02/01 00:42
MHDA- 2024/02/19 06:42
CRDT- 2024/01/31 18:13
PHST- 2023/10/24 00:00 [received]
PHST- 2024/01/08 00:00 [revised]
PHST- 2024/01/14 00:00 [accepted]
PHST- 2024/02/19 06:42 [medline]
PHST- 2024/02/01 00:42 [pubmed]
PHST- 2024/01/31 18:13 [entrez]
AID - S1570-0232(24)00021-7 [pii]
AID - 10.1016/j.jchromb.2024.124013 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2024 Feb 15;1234:124013. doi: 
      10.1016/j.jchromb.2024.124013. Epub 2024 Jan 26.