PMID- 38296626
OWN - NLM
STAT- MEDLINE
DCOM- 20240202
LR  - 20240228
IS  - 1999-6187 (Electronic)
IS  - 1009-3419 (Print)
IS  - 1009-3419 (Linking)
VI  - 27
IP  - 1
DP  - 2024 Jan 20
TI  - [Establishment of a 21-color Panel for the Detection of Immune Cell Subsets 
in 
      Human Non-small Cell Lung Cancer Tumor Tissues with Flow Cytometry].
PG  - 56-64
LID - 10.3779/j.issn.1009-3419.2024.102.02 [doi]
AB  - BACKGROUND: With the rise of multicolor flow cytometry, flow cytometry has become 
      an important means to detect the immune microenvironment of lung cancer, but most 
      of them are used to detect the proportion of cell subsets or the function of 
      major cell subsets, and they cannot be detected at the same time. Therefore, a 
      reliable 21-color flow cytometry protocol was established to detect the immune 
      cell subsets in human non-small cell lung cancer (NSCLC) tumor tissues. METHODS: 
      Cell membrane surface antibodies cluster of differentiation (CD)45, CD3, CD19, 
      CD4, CD8, programmed cell death 1 (PD-1), CD39, CD103, CD25, CD127, chemokine 
      receptor 8 (CCR8), CD56, CD11c, human leukocyte antigen (HLA)-DR, CD38, CD27, 
      CD69, CD62L, CD45RA, CCR7 and nucleic acid dye L/D were used to develop the 
      protocol. Firstly, antibody titration experiments, voltage optimization, 
      subtraction of one color staining and single color staining experiments were 
      carried out for each antibody, and after the experimental conditions and 
      detection schemes were determined, the feasibility of the scheme was verified by 
      using peripheral blood mononuclear cells (PBMCs) specimens of six healthy adult 
      volunteers. Tumor tissue samples from 6 NSCLC patients were tested and analyzed. 
      RESULTS: The established 21-color flow cytometry protocol was used to detect the 
      tumor tissue samples of 6 NSCLC patients, and the proportion of each cell subset 
      in lung cancer tissue, as well as the immunophenotype and differentiation of the 
      main cell population, were analyzed. CONCLUSIONS: The successfully established 
      21-color flow cytometry protocol is suitable for the detection of PBMCs and NSCLC 
      tissue samples, which provides an effective new idea for monitoring the immune 
      microenvironment status in lung cancer.
FAU - Guo, Tingting
AU  - Guo T
AD  - College of Ecology and Environment, Chengdu University of Technology, Chengdu 
      610059, China.
AD  - Institute of Respiratory Health, West China Hospital, Sichuan University, Chengdu 
      610041, China.
FAU - Xie, Hongguan
AU  - Xie H
AD  - College of Ecology and Environment, Chengdu University of Technology, Chengdu 
      610059, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Fei Ai Za Zhi
JT  - Zhongguo fei ai za zhi = Chinese journal of lung cancer
JID - 101126433
SB  - IM
MH  - Adult
MH  - Humans
MH  - *Carcinoma, Non-Small-Cell Lung/pathology
MH  - *Lung Neoplasms/pathology
MH  - Flow Cytometry
MH  - Leukocytes, Mononuclear/metabolism/pathology
MH  - Lung/pathology
MH  - Tumor Microenvironment
PMC - PMC10895290
OTO - NOTNLM
OT  - Immunophenotype
OT  - Lung neoplasms
OT  - Multicolor flow cytometry
OT  - Peripheral blood mononuclear cells
COIS- Competing interests: The authors declare that they have no competing interests.
EDAT- 2024/02/01 00:42
MHDA- 2024/02/02 06:43
PMCR- 2024/01/20
CRDT- 2024/01/31 21:37
PHST- 2024/02/02 06:43 [medline]
PHST- 2024/02/01 00:42 [pubmed]
PHST- 2024/01/31 21:37 [entrez]
PHST- 2024/01/20 00:00 [pmc-release]
AID - 10.3779/j.issn.1009-3419.2024.102.02 [doi]
PST - ppublish
SO  - Zhongguo Fei Ai Za Zhi. 2024 Jan 20;27(1):56-64. doi: 
      10.3779/j.issn.1009-3419.2024.102.02.