PMID- 38302672
OWN - NLM
STAT- MEDLINE
DCOM- 20240205
LR  - 20240206
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 14
IP  - 1
DP  - 2024 Feb 1
TI  - Analysis of m6A regulators related immune characteristics in ankylosing 
      spondylitis by integrated bioinformatics and computational strategies.
PG  - 2724
LID - 10.1038/s41598-024-53184-z [doi]
LID - 2724
AB  - N6-methyladenosine (m6A) modification, as a common epigenetic modification, has 
      been widely studied in autoimmune diseases. However, the role of m6A in the 
      regulation of the immune microenvironment of ankylosing spondylitis (AS) remains 
      unclear. Therefore, we aimed to investigate the effect of m6A modification on the 
      immune microenvironment of AS. We first evaluated RNA modification patterns 
      mediated by 26 m6A regulators in 52 AS samples and 20 healthy samples. 
      Thereafter, an m6A related classifier composed of seven genes was constructed and 
      could effectively distinguish healthy and AS samples. Then, the correlation 
      between m6A regulators and immune characteristics were investigated, including 
      infiltrating immunocytes, immune reactions activity, and human leukocyte antigen 
      (HLA) genes expression. The results indicated that m6A regulators was closely 
      correlated with immune characteristics. For example, EIF3A was significantly 
      related to infiltrating immunocytes; IGF2BP2 and EIF3A were significant 
      regulators in immune reaction of TGF-beta family member, and the expression of 
      HLA-DPA1 and HLA-E were affected by EIF3A and ALKBH5. Next, two distinct m6A 
      expression patterns were identified through unsupervised clustering analysis, and 
      diverse immune characteristics were found between them. A total of 5889 m6A 
      phenotype-related genes were obtained between the two expression patterns, and 
      their biological functions were revealed. Finally, we validated the expression 
      status of m6A modification regulators using two additional datasets. Our findings 
      illustrate that m6A modifications play a critical role in the diversity and 
      complexity of the AS immune microenvironment.
CI  - (c) 2024. The Author(s).
FAU - Guo, Da
AU  - Guo D
AD  - Osteonecrosis and Joint Reconstruction Ward, Department of Joint Surgery, HongHui 
      Hospital, Xi'an Jiaotong University, Xi'an, 710054, Shaanxi, China.
FAU - Liu, Jiayi
AU  - Liu J
AD  - Xinglin College, Liaoning University of Traditional Chinese Medicine, Shenyang, 
      110167, Liaoning, China.
FAU - Li, Shuang
AU  - Li S
AD  - Department of Neurology, Tangdu Hospital, The Fourth Military Medical University, 
      Xi'an, 710038, Shaanxi, China.
FAU - Xu, Peng
AU  - Xu P
AD  - Osteonecrosis and Joint Reconstruction Ward, Department of Joint Surgery, HongHui 
      Hospital, Xi'an Jiaotong University, Xi'an, 710054, Shaanxi, China. 
      sousou369@163.com.
LA  - eng
PT  - Journal Article
DEP - 20240201
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - W7IBY2BGAX (6-methyladenine)
RN  - JAC85A2161 (Adenine)
RN  - 0 (IGF2BP2 protein, human)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - Humans
MH  - *Spondylitis, Ankylosing/genetics
MH  - *Autoimmune Diseases
MH  - Adenine
MH  - Computational Biology
MH  - RNA-Binding Proteins
PMC - PMC10834589
COIS- The authors declare no competing interests.
EDAT- 2024/02/02 00:42
MHDA- 2024/02/05 06:42
PMCR- 2024/02/01
CRDT- 2024/02/01 23:48
PHST- 2023/06/09 00:00 [received]
PHST- 2024/01/29 00:00 [accepted]
PHST- 2024/02/05 06:42 [medline]
PHST- 2024/02/02 00:42 [pubmed]
PHST- 2024/02/01 23:48 [entrez]
PHST- 2024/02/01 00:00 [pmc-release]
AID - 10.1038/s41598-024-53184-z [pii]
AID - 53184 [pii]
AID - 10.1038/s41598-024-53184-z [doi]
PST - epublish
SO  - Sci Rep. 2024 Feb 1;14(1):2724. doi: 10.1038/s41598-024-53184-z.