PMID- 38303002
OWN - NLM
STAT- MEDLINE
DCOM- 20240206
LR  - 20240206
IS  - 2662-7671 (Electronic)
IS  - 2662-7671 (Linking)
VI  - 24
IP  - 1
DP  - 2024 Feb 1
TI  - Melissa officinalis extract palliates redox imbalance and inflammation associated 
      with hyperthyroidism-induced liver damage by regulating Nrf-2/ Keap-1 gene 
      expression in gamma-irradiated rats.
PG  - 71
LID - 10.1186/s12906-024-04370-z [doi]
LID - 71
AB  - BACKGROUND: Melissa officinalis (MO) is a well-known medicinal plant species used 
      in the treatment of several diseases; it is widely used as a vegetable, adding 
      flavour to dishes. This study was designed to evaluate the therapeutic effect of 
      MO Extract against hyperthyroidism induced by Eltroxin and gamma-radiation. METHODS: 
      Hyperthyroidism was induced by injecting rats with Eltroxin (100 microg/kg/ day) for 
      14 days and exposure to gamma-radiation (IR) (5 Gy single dose). The hyperthyroid 
      rats were orally treated with MO extract (75 mg/kg/day) at the beginning of the 
      second week of the Eltroxin injection and continued for another week. The levels 
      of thyroid hormones, liver enzymes and proteins besides the impaired hepatic 
      redox status and antioxidant parameters were measured using commercial kits. The 
      hepatic gene expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and 
      its inhibitor Kelch-like ECH-associated protein-1(Keap-1) in addition to hepatic 
      inflammatory mediators including tumor necrosis factor-alpha (TNF- alpha), Monocyte 
      chemoattractant protein-1 (MCP-1) and fibrogenic markers such as transforming 
      growth factor-beta1 (TGF-beta1) were determined. RESULTS: MO Extract reversed the 
      effect of Eltroxin + IR on rats and attenuated the thyroid hormones. Moreover, it 
      alleviated hyperthyroidism-induced hepatic damage by inhibiting the hepatic 
      enzymes' activities as well as enhancing the production of proteins concomitant 
      with improving cellular redox homeostasis by attenuating the deranged redox 
      balance and modulating the Nrf2/Keap-1 pathway. Additionally, MO Extract 
      alleviated the inflammatory response by suppressing the TNF- alpha and MCP-1 and 
      prevented hepatic fibrosis via Nrf2-mediated inhibition of the TGF-beta1/Smad 
      pathway. CONCLUSION: Accordingly, these results might strengthen the 
      hepatoprotective effect of MO Extract in a rat model of hyperthyroidism by 
      regulating the Nrf-2/ Keap-1 pathway.
CI  - (c) 2024. The Author(s).
FAU - Kawara, Ragaa Sm
AU  - Kawara RS
AD  - Biochemistry and Molecular Biology Department, Faculty of Vet. Med, Benha 
      University, Banha, Egypt.
FAU - Moawed, Fatma Sm
AU  - Moawed FS
AUID- ORCID: 0000-0002-5792-0815
AD  - Health radiation research, National Center for Radiation Research and Technology, 
      Egyptian Atomic Energy Authority, Nasr City, 11787, Cairo, Egypt.
FAU - Elsenosi, Yakout
AU  - Elsenosi Y
AD  - Biochemistry and Molecular Biology Department, Faculty of Vet. Med, Benha 
      University, Banha, Egypt.
FAU - Elmaksoud, Hussein Abd
AU  - Elmaksoud HA
AD  - Biochemistry and Molecular Biology Department, Faculty of Vet. Med, Benha 
      University, Banha, Egypt.
FAU - Ahmed, Esraa S A
AU  - Ahmed ESA
AUID- ORCID: 0000-0001-5358-4394
AD  - Radiation Biology Research, National Center for Radiation Research and 
      Technology, Egyptian Atomic Energy Authority, Nasr City, 11787, Cairo, Egypt. 
      esraa.tamim@yahoo.com.
FAU - Abo-Zaid, Omayma Ar
AU  - Abo-Zaid OA
AUID- ORCID: 0000-0002-1752-6709
AD  - Biochemistry and Molecular Biology Department, Faculty of Vet. Med, Benha 
      University, Banha, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20240201
PL  - England
TA  - BMC Complement Med Ther
JT  - BMC complementary medicine and therapies
JID - 101761232
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Plant Extracts)
RN  - 0 (Thyroid Hormones)
RN  - Q51BO43MG4 (Thyroxine)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Animals
MH  - Rats
MH  - Gene Expression
MH  - *Hyperthyroidism/complications/drug therapy
MH  - Inflammation/metabolism
MH  - Liver
MH  - *Melissa/chemistry
MH  - NF-E2-Related Factor 2/metabolism
MH  - Oxidation-Reduction
MH  - *Plant Extracts/pharmacology/therapeutic use
MH  - Thyroid Hormones/metabolism
MH  - Thyroxine/genetics/metabolism
MH  - Transforming Growth Factor beta1/metabolism
MH  - *Liver Diseases/etiology/therapy
PMC - PMC10832092
OTO - NOTNLM
OT  - Hyperthyroidism
OT  - Inflammation
OT  - Liver damage
OT  - Melissa officinalis
OT  - Nrf2/Keap1
COIS- The authors declare no competing interests.
EDAT- 2024/02/02 06:43
MHDA- 2024/02/05 06:43
PMCR- 2024/02/01
CRDT- 2024/02/02 00:07
PHST- 2023/10/22 00:00 [received]
PHST- 2024/01/22 00:00 [accepted]
PHST- 2024/02/05 06:43 [medline]
PHST- 2024/02/02 06:43 [pubmed]
PHST- 2024/02/02 00:07 [entrez]
PHST- 2024/02/01 00:00 [pmc-release]
AID - 10.1186/s12906-024-04370-z [pii]
AID - 4370 [pii]
AID - 10.1186/s12906-024-04370-z [doi]
PST - epublish
SO  - BMC Complement Med Ther. 2024 Feb 1;24(1):71. doi: 10.1186/s12906-024-04370-z.