PMID- 38306432
OWN - NLM
STAT- MEDLINE
DCOM- 20240205
LR  - 20240206
IS  - 2375-2548 (Electronic)
IS  - 2375-2548 (Linking)
VI  - 10
IP  - 5
DP  - 2024 Feb 2
TI  - T-FINDER: A highly sensitive, pan-HLA platform for functional T cell receptor and 
      ligand discovery.
PG  - eadk3060
LID - 10.1126/sciadv.adk3060 [doi]
LID - eadk3060
AB  - Effective, unbiased, high-throughput methods to functionally identify both class 
      II and class I HLA-presented T cell epitopes and their cognate T cell receptors 
      (TCRs) are essential for and prerequisite to diagnostic and therapeutic 
      applications, yet remain underdeveloped. Here, we present T-FINDER [T cell 
      Functional Identification and (Neo)-antigen Discovery of Epitopes and Receptors], 
      a system to rapidly deconvolute CD4 and CD8 TCRs and targets physiologically 
      processed and presented by an individual's unmanipulated, complete human 
      leukocyte antigen (HLA) haplotype. Combining a highly sensitive TCR signaling 
      reporter with an antigen processing system to overcome previously undescribed 
      limitations to target expression, T-FINDER both robustly identifies unknown 
      peptide:HLA ligands from antigen libraries and rapidly screens and functionally 
      validates the specificity of large TCR libraries against known or predicted 
      targets. To demonstrate its capabilities, we apply the platform to multiple 
      TCR-based applications, including diffuse midline glioma, celiac disease, and 
      rheumatoid arthritis, providing unique biological insights and showcasing 
      T-FINDER's potency and versatility.
FAU - Cetin, Miray
AU  - Cetin M
AUID- ORCID: 0009-0001-7711-0211
AD  - BioMed X GmbH, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.
FAU - Pinamonti, Veronica
AU  - Pinamonti V
AUID- ORCID: 0000-0001-5253-095X
AD  - BioMed X GmbH, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.
AD  - Faculty of Biosciences, Heidelberg University, 69120 Heidelberg, Germany.
FAU - Schmid, Theresa
AU  - Schmid T
AUID- ORCID: 0009-0007-4796-6776
AD  - BioMed X GmbH, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.
FAU - Boschert, Tamara
AU  - Boschert T
AUID- ORCID: 0009-0007-8957-5319
AD  - Faculty of Biosciences, Heidelberg University, 69120 Heidelberg, Germany.
AD  - DKTK CCU Neuroimmunology and Brain Tumor Immunology, German Cancer Research 
      Center (DKFZ), 69120 Heidelberg, Germany.
AD  - Helmoltz Institute for Translational Oncology (HI-TRON), Heidelberg, Germany.
FAU - Mellado Fuentes, Ana
AU  - Mellado Fuentes A
AUID- ORCID: 0009-0000-9193-1430
AD  - BioMed X GmbH, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.
FAU - Kromer, Kristina
AU  - Kromer K
AUID- ORCID: 0009-0005-5617-7635
AD  - BioMed X GmbH, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.
AD  - Faculty of Biosciences, Heidelberg University, 69120 Heidelberg, Germany.
FAU - Lerner, Taga
AU  - Lerner T
AD  - BioMed X GmbH, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.
FAU - Zhang, Jing
AU  - Zhang J
AD  - BioMed X GmbH, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.
FAU - Herzig, Yonatan
AU  - Herzig Y
AD  - BioMed X GmbH, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.
FAU - Ehlert, Christopher
AU  - Ehlert C
AD  - Heidelberg Institute for Theoretical Studies (HITS gGmbH), 69118 Heidelberg, 
      Germany.
FAU - Hernandez-Hernandez, Miguel
AU  - Hernandez-Hernandez M
AUID- ORCID: 0000-0001-8503-1954
AD  - BioMed X GmbH, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.
FAU - Samaras, Georgios
AU  - Samaras G
AUID- ORCID: 0000-0002-1538-0048
AD  - BioMed X GmbH, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.
FAU - Torres, Claudia Maldonado
AU  - Torres CM
AD  - BioMed X GmbH, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.
FAU - Fisch, Laura
AU  - Fisch L
AUID- ORCID: 0000-0002-0529-3186
AD  - BioMed X GmbH, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.
AD  - Faculty of Biosciences, Heidelberg University, 69120 Heidelberg, Germany.
FAU - Dragan, Valeriia
AU  - Dragan V
AUID- ORCID: 0009-0008-7173-8199
AD  - BioMed X GmbH, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.
AD  - Faculty of Biosciences, Heidelberg University, 69120 Heidelberg, Germany.
FAU - Kouwenhoven, Arlette
AU  - Kouwenhoven A
AD  - Janssen Research and Development, Beerse, Belgium.
FAU - Van Schoubroeck, Bertrand
AU  - Van Schoubroeck B
AUID- ORCID: 0009-0000-1798-6927
AD  - Janssen Research and Development, Beerse, Belgium.
FAU - Wils, Hans
AU  - Wils H
AD  - Janssen Research and Development, Beerse, Belgium.
FAU - Van Hove, Carl
AU  - Van Hove C
AUID- ORCID: 0009-0002-9271-5689
AD  - Janssen Research and Development, Beerse, Belgium.
FAU - Platten, Michael
AU  - Platten M
AUID- ORCID: 0000-0002-4746-887X
AD  - DKTK CCU Neuroimmunology and Brain Tumor Immunology, German Cancer Research 
      Center (DKFZ), 69120 Heidelberg, Germany.
AD  - Helmoltz Institute for Translational Oncology (HI-TRON), Heidelberg, Germany.
AD  - Department of Neurology, Medical Faculty Mannheim, MCTN Heidelberg University, 
      Mannheim, Germany.
FAU - Green, Edward W
AU  - Green EW
AUID- ORCID: 0000-0003-1922-2298
AD  - DKTK CCU Neuroimmunology and Brain Tumor Immunology, German Cancer Research 
      Center (DKFZ), 69120 Heidelberg, Germany.
AD  - Department of Neurology, Medical Faculty Mannheim, MCTN Heidelberg University, 
      Mannheim, Germany.
FAU - Stevenaert, Frederik
AU  - Stevenaert F
AD  - Janssen Research and Development, Beerse, Belgium.
FAU - Felix, Nathan J
AU  - Felix NJ
AD  - Janssen Research and Development, Spring House, PA, USA.
FAU - Lindner, John M
AU  - Lindner JM
AUID- ORCID: 0000-0002-4940-5354
AD  - BioMed X GmbH, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.
LA  - eng
PT  - Journal Article
DEP - 20240202
PL  - United States
TA  - Sci Adv
JT  - Science advances
JID - 101653440
RN  - 0 (Ligands)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
CIN - doi: 10.1126/sciadv.adi9091
MH  - Humans
MH  - Ligands
MH  - *Histocompatibility Antigens Class I
MH  - *Receptors, Antigen, T-Cell/metabolism
MH  - HLA Antigens
MH  - Histocompatibility Antigens Class II
PMC - PMC10836725
EDAT- 2024/02/02 18:42
MHDA- 2024/02/05 06:42
PMCR- 2024/02/02
CRDT- 2024/02/02 14:05
PHST- 2024/02/05 06:42 [medline]
PHST- 2024/02/02 18:42 [pubmed]
PHST- 2024/02/02 14:05 [entrez]
PHST- 2024/02/02 00:00 [pmc-release]
AID - adk3060 [pii]
AID - 10.1126/sciadv.adk3060 [doi]
PST - ppublish
SO  - Sci Adv. 2024 Feb 2;10(5):eadk3060. doi: 10.1126/sciadv.adk3060. Epub 2024 Feb 2.