PMID- 38307565
OWN - NLM
STAT- MEDLINE
DCOM- 20240205
LR  - 20240214
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 44
IP  - 2
DP  - 2024 Feb
TI  - Effects of Extended Fixation on Advanced Gastric Cancer HER2 Status Assessment 
      Using IHC and FISH.
PG  - 621-630
LID - 10.21873/anticanres.16851 [doi]
AB  - BACKGROUND/AIM: In gastric cancer, accurate determination of human epidermal 
      growth factor receptor type 2 (HER2) status is crucial for treatment 
      decision-making. However, the optimal formalin fixation time of gastric cancer 
      specimens for HER2 status determination remains unestablished. Here, we 
      investigated real-world data on formalin overfixation and its effect on HER2 
      status determination in gastric cancer. PATIENTS AND METHODS: We comprehensively 
      analyzed HER2 testing results in 228 gastric cancer specimens, including those 
      subjected to formalin overfixation. Subsequently, we divided 52 resected 
      specimens of advanced gastric cancer into three groups and studied the effects of 
      short-term (6-72 h) and long-term (1 and 2 weeks) fixation on HER2 status 
      determination using immunohistochemistry (IHC) and fluorescence in situ 
      hybridization (FISH). RESULTS: A total of 21.5% (49/228) of the specimens were 
      HER2-positive, whereas 78.5% (179/228) were negative. Among the HER2-negative 
      specimens, no biopsies were overfixed, whereas 12.5% (9/72) of the surgical 
      resection specimens were overfixed. The HER2 status of the 6-72-h group was 82.7% 
      and 76.9% identical to that of the 1- and 2-week groups, when determined using 
      IHC, and 73.1% and 36.5%, when determined using FISH, respectively. However, HER2 
      determination was not feasible in 26.9% and 63.5% of the specimens in the 1- and 
      2-week groups, respectively. CONCLUSION: Formalin overfixation may hinder the 
      determination of HER2 status and should be avoided in gastric cancer sample 
      preparation.
CI  - Copyright (c) 2024 International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Kondo, Junya
AU  - Kondo J
AD  - Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi 
      University Graduate School of Medicine, Ube, Japan.
AD  - Department of Surgery, National Hospital Organization Kanmon Medical Center, 
      Shimonoseki, Japan.
FAU - Yoshino, Shigefumi
AU  - Yoshino S
AD  - Department of Surgery, National Hospital Organization Kanmon Medical Center, 
      Shimonoseki, Japan.
FAU - Iida, Michihisa
AU  - Iida M
AD  - Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi 
      University Graduate School of Medicine, Ube, Japan.
FAU - Takeda, Shigeru
AU  - Takeda S
AD  - Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi 
      University Graduate School of Medicine, Ube, Japan.
FAU - Nakashima, Chiyo
AU  - Nakashima C
AD  - Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi 
      University Graduate School of Medicine, Ube, Japan.
FAU - Watanabe, Yusaku
AU  - Watanabe Y
AD  - Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi 
      University Graduate School of Medicine, Ube, Japan.
FAU - Nishiyama, Mitsuo
AU  - Nishiyama M
AD  - Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi 
      University Graduate School of Medicine, Ube, Japan.
FAU - Tokumitsu, Yukio
AU  - Tokumitsu Y
AD  - Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi 
      University Graduate School of Medicine, Ube, Japan.
FAU - Shindo, Yoshitaro
AU  - Shindo Y
AD  - Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi 
      University Graduate School of Medicine, Ube, Japan.
FAU - Nishimura, Taku
AU  - Nishimura T
AD  - Department of Gastroenterological Surgery, Japan Community Health Care 
      Organization Shimonoseki Medical Center, Shimonoseki, Japan.
FAU - Suzuki, Nobuaki
AU  - Suzuki N
AD  - Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi 
      University Graduate School of Medicine, Ube, Japan.
FAU - Hoshii, Yoshinobu
AU  - Hoshii Y
AD  - Department of Diagnostic Pathology, Yamaguchi University Hospital, Yamaguchi, 
      Japan.
FAU - Itoh, Hiroshi
AU  - Itoh H
AD  - Department of Molecular Pathology, Yamaguchi University Graduate School of 
      Medicine, Yamaguchi, Japan.
FAU - Nagano, Hiroaki
AU  - Nagano H
AD  - Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi 
      University Graduate School of Medicine, Ube, Japan; hnagano@yamaguchi-u.ac.jp.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Humans
MH  - *Biomarkers, Tumor/genetics/metabolism
MH  - Immunohistochemistry
MH  - *Stomach Neoplasms/genetics/surgery/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Receptor, ErbB-2/genetics/metabolism
MH  - Formaldehyde
OTO - NOTNLM
OT  - FISH analysis
OT  - HER2
OT  - formalin fixation
OT  - gastric cancer
OT  - immunohistochemistry
OT  - trastuzumab
EDAT- 2024/02/03 00:42
MHDA- 2024/02/05 06:42
CRDT- 2024/02/02 20:34
PHST- 2023/10/26 00:00 [received]
PHST- 2023/12/19 00:00 [revised]
PHST- 2023/12/20 00:00 [accepted]
PHST- 2024/02/05 06:42 [medline]
PHST- 2024/02/03 00:42 [pubmed]
PHST- 2024/02/02 20:34 [entrez]
AID - 44/2/621 [pii]
AID - 10.21873/anticanres.16851 [doi]
PST - ppublish
SO  - Anticancer Res. 2024 Feb;44(2):621-630. doi: 10.21873/anticanres.16851.