PMID- 38311009
OWN - NLM
STAT- MEDLINE
DCOM- 20240226
LR  - 20240417
IS  - 1872-7905 (Electronic)
IS  - 0022-1759 (Linking)
VI  - 526
DP  - 2024 Mar
TI  - Comparison of three methods for the detection of antibodies against 
      muscle-specific kinase.
PG  - 113627
LID - S0022-1759(24)00012-7 [pii]
LID - 10.1016/j.jim.2024.113627 [doi]
AB  - OBJECTIVES: To compare 3 different methods for the detection of antibodies 
      against muscle-specific kinase (MuSK). METHODS: MuSK antibody testing was 
      performed in 237 serum samples by enzyme-linked immunosorbent assay (ELISA) and 
      fixed cell-based assay (f-CBA-IFA). One hundred and forty-eight (148) of the sera 
      had previously been tested by RIA during clinical testing: 47 MuSK antibody 
      positive and 101 MuSK antibody negative. Of the MuSK RIA negative antibodies, 46 
      tested positive for other neural antibodies. Additionally, 89 sera were 
      subsequently tested by all three methods: 70 healthy controls and 19 sera 
      positive for other neural antibodies. RESULTS: Qualitative inter-assay agreement 
      based on tiered RIA values was 100% for results of 1.00 nmol/L or greater by both 
      methods; 81% and 94% for results between 0.21 and 0.99 nmol/L by ELISA and 
      f-CBA-IFA, respectively; and 0% for results of 0.04-0.20 nmol/L by both methods. 
      Negative results showed 100% agreement between RIA and both ELISA and f-CBA-IFA 
      (n = 55). None of the controls positive for other neural autoantibodies or 
      healthy controls were positive in any assay. CONCLUSION: Overall, excellent 
      agreement was observed between the 3 methods used to detect antibodies against 
      MuSK. Both the f-CBA-IFA and ELISA performed comparably to RIA and exhibited 
      excellent overall accuracy for MuSK IgG detection, with the f-CBA-IFA 
      demonstrating higher agreement between positive samples with the RIA than the 
      ELISA without identifying false positives in the control samples. Advantages of 
      non-radioactive methods for the detection of MuSK antibodies include reduced 
      handling and disposal of hazardous materials, potential for automation and the 
      reagents having a longer shelf-life, reducing costs associated with both workflow 
      and lot validations. Thus, commercially available ELISA and transfected 
      cell-based assays are viable alternatives to the traditional radioactive assay 
      used for serologic determination of MuSK IgG.
CI  - Copyright (c) 2024 Elsevier B.V. All rights reserved.
FAU - Luong, Kyphuong
AU  - Luong K
AD  - ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt 
      Lake City, UT 84108, USA.
FAU - Lozier, Bucky K
AU  - Lozier BK
AD  - ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt 
      Lake City, UT 84108, USA.
FAU - Novis, Camille L
AU  - Novis CL
AD  - ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt 
      Lake City, UT 84108, USA.
FAU - Smith, Tammy L
AU  - Smith TL
AD  - ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt 
      Lake City, UT 84108, USA; Department of Pathology, University of Utah School of 
      Medicine, 15 N Medical Dr. East Ste. 1100, Salt Lake City, UT 84112, USA; 
      Geriatric Research Education and Clinical Center, George E. Whalen Department of 
      Veterans Affairs Medical Center, Salt Lake City, UT 84148, USA; Department of 
      Neurology, University of Utah School of Medicine, Salt Lake City, UT 84132, USA; 
      Neurology Service, George E. Whalen Department of Veterans Affairs Medical 
      Center, Salt Lake City, UT 84148, USA.
FAU - Zuromski, Lauren M
AU  - Zuromski LM
AD  - ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt 
      Lake City, UT 84108, USA.
FAU - Peterson, Lisa K
AU  - Peterson LK
AD  - ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt 
      Lake City, UT 84108, USA; Department of Pathology, University of Utah School of 
      Medicine, 15 N Medical Dr. East Ste. 1100, Salt Lake City, UT 84112, USA. 
      Electronic address: lisa.k.peterson@aruplab.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20240203
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Receptors, Cholinergic)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - 0 (Autoantibodies)
RN  - 0 (Antibodies, Monoclonal, Humanized)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Humans
MH  - *Myasthenia Gravis
MH  - Receptors, Cholinergic
MH  - Receptor Protein-Tyrosine Kinases
MH  - Autoantibodies
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Antibodies, Monoclonal, Humanized
MH  - Immunoglobulin G
MH  - Muscles
OTO - NOTNLM
OT  - Autoimmune disorder
OT  - Cell-based assay
OT  - ELISA
OT  - Myasthenia gravis
OT  - Radioimmunoassay
COIS- Declaration of competing interest Reagents used for the detection of MuSK 
      antibodies by CBA were provided free of charge by Euroimmun. LKP is a member of 
      the Werfen Autoimmune Disease Strategic Advisory Council and has received 
      associated travel and consulting fees for work unrelated to this manuscript. TLS 
      receives research funding from Alexion Pharmaceuticals for work unrelated to this 
      manuscript and has received travel support from Euroimmun US to attend the 
      Euroimmun Academy. KL, BKL, CLN and LZ do not have any other declarations of 
      interest.
EDAT- 2024/02/05 00:42
MHDA- 2024/02/26 06:43
CRDT- 2024/02/04 19:27
PHST- 2023/11/01 00:00 [received]
PHST- 2024/02/01 00:00 [revised]
PHST- 2024/02/01 00:00 [accepted]
PHST- 2024/02/26 06:43 [medline]
PHST- 2024/02/05 00:42 [pubmed]
PHST- 2024/02/04 19:27 [entrez]
AID - S0022-1759(24)00012-7 [pii]
AID - 10.1016/j.jim.2024.113627 [doi]
PST - ppublish
SO  - J Immunol Methods. 2024 Mar;526:113627. doi: 10.1016/j.jim.2024.113627. Epub 2024 
      Feb 3.