PMID- 38316232
OWN - NLM
STAT- MEDLINE
DCOM- 20240301
LR  - 20240301
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 154
DP  - 2024 May
TI  - The effects of four paralogous piscidin antimicrobial peptides on the chemotaxis, 
      macrophage respiratory burst, phagocytosis and expression of immune-related genes 
      in orange-spotted grouper (Epinephelus coicodes).
PG  - 105144
LID - S0145-305X(24)00016-8 [pii]
LID - 10.1016/j.dci.2024.105144 [doi]
AB  - Antimicrobial peptides (AMPs) are an essential part of the vertebrate innate 
      immune system. Piscidins are a family of AMPs specific in fish. In our previous 
      investigation, we identified four paralogous genes of piscidins in the 
      orange-spotted grouper (Epinephelus coicodes), which exhibited distinct 
      activities against bacteria, fungi, and parasitic ciliated protozoa. Piscidins 
      demonstrated their capability to modulate the expression of diverse 
      immune-related genes; however, their precise immunoregulatory functions remain 
      largely unexplored. In this study, we examined the immunomodulatory properties of 
      putative mature peptides derived from four E. coicodes piscidins (ecPis1S, 
      ecPis2S, ecPis3S, and ecPis4S) in head kidney leukocytes (HKLs) or 
      monocytes/macrophages (MO/MPhi)-like cells isolated from E. coicodes. Our data 
      demonstrate that E. coicodes piscidins exhibit immunomodulatory activities 
      supported by multiple lines of evidence. Firstly, all four piscidins displayed 
      chemotactic activities towards HKLs, with the most potent chemotactic activity 
      observed in ecPis2S. Secondly, stimulation with E. coicodes piscidins enhanced 
      respiratory burst and phagocytic activity in MO/Mcapital EF, Cyrillic-like cells, with ecPis3S 
      showing the highest efficacy in increasing phagocytosis of MO/MPhi-like cells. 
      Thirdly, mRNA expression levels of chemokine receptors, Toll-like receptors, T 
      cell receptors, and proinflammatory cytokines were modulated to varying extents 
      by the four piscidins in E. coicodes HKLs. Overall, our findings indicate that 
      the immunological activities of these four paralogous piscidins from E. coicodes 
      are exhibited in a paralog-specific and concentration-dependent manner, 
      highlighting their distinct and versatile immunomodulatory properties. This study 
      makes a significant contribution to the field of fish AMPs immunology by 
      elucidating the novel mechanisms through which members of the piscidin family 
      exert their immunomodulatory effects. Moreover, it provides valuable insights for 
      further exploration of fish immunomodulating agents.
CI  - Copyright (c) 2024 Elsevier Ltd. All rights reserved.
FAU - Huang, Xiazi
AU  - Huang X
AD  - Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, 
      Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life 
      Sciences and Oceanography, Shenzhen University, Shenzhen, 518055, China.
FAU - Mao, Wei
AU  - Mao W
AD  - Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, 
      Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life 
      Sciences and Oceanography, Shenzhen University, Shenzhen, 518055, China.
FAU - Yi, Yonghao
AU  - Yi Y
AD  - Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, 
      Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life 
      Sciences and Oceanography, Shenzhen University, Shenzhen, 518055, China.
FAU - Lu, Yubin
AU  - Lu Y
AD  - Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, 
      Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life 
      Sciences and Oceanography, Shenzhen University, Shenzhen, 518055, China.
FAU - Liu, Feihong
AU  - Liu F
AD  - Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, 
      Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life 
      Sciences and Oceanography, Shenzhen University, Shenzhen, 518055, China.
FAU - Deng, Li
AU  - Deng L
AD  - Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, 
      Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life 
      Sciences and Oceanography, Shenzhen University, Shenzhen, 518055, China. 
      Electronic address: lideng03@szu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20240203
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Antimicrobial Peptides)
RN  - 0 (Antimicrobial Cationic Peptides)
RN  - 0 (Fish Proteins)
SB  - IM
MH  - Animals
MH  - *Bass/genetics/metabolism
MH  - Amino Acid Sequence
MH  - Antimicrobial Peptides
MH  - Chemotaxis
MH  - Respiratory Burst
MH  - Antimicrobial Cationic Peptides/metabolism
MH  - Sequence Alignment
MH  - Fish Proteins/metabolism
MH  - Macrophages/metabolism
MH  - Phagocytosis
OTO - NOTNLM
OT  - Antimicrobial peptide
OT  - Chemotaxis
OT  - Epinephelus coicodes
OT  - Gene expression
OT  - Phagocytosis
OT  - Piscidin
COIS- Declaration of competing interest The authors declare that they have no conflicts 
      of interest.
EDAT- 2024/02/06 00:42
MHDA- 2024/03/01 06:44
CRDT- 2024/02/05 19:22
PHST- 2023/08/30 00:00 [received]
PHST- 2024/01/09 00:00 [revised]
PHST- 2024/02/02 00:00 [accepted]
PHST- 2024/03/01 06:44 [medline]
PHST- 2024/02/06 00:42 [pubmed]
PHST- 2024/02/05 19:22 [entrez]
AID - S0145-305X(24)00016-8 [pii]
AID - 10.1016/j.dci.2024.105144 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2024 May;154:105144. doi: 10.1016/j.dci.2024.105144. Epub 2024 
      Feb 3.