PMID- 38316940
OWN - NLM
STAT- MEDLINE
DCOM- 20240207
LR  - 20240210
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 14
IP  - 1
DP  - 2024 Feb 5
TI  - NRF2 attenuation aggravates detrimental consequences of metabolic stress on 
      cultured porcine parthenote embryos.
PG  - 2973
LID - 10.1038/s41598-024-53480-8 [doi]
LID - 2973
AB  - The nuclear factor erythroid 2-related factor 2 (NRF2) is a crucial transcription 
      factor that plays a central role in regulating oxidative stress pathways by 
      binding antioxidant response elements, but its involvement in early embryo 
      development remains largely unexplored. In this study, we demonstrated that NRF2 
      mRNA is expressed in porcine embryos from day 2 to day 7 of development, showing 
      a decrease in abundance from day 2 to day 3, followed by an increase on day 5 and 
      day 7. Comparable levels of NRF2 mRNA were observed between early-cleaving and 
      more developmental competent embryos and late-cleaving and less developmental 
      competent embryos on day 4 and day 5 of culture. Attenuation of NRF2 mRNA 
      significantly decreased development of parthenote embryos to the blastocyst 
      stage. When NRF2-attenuated embryos were cultured in presence of 3.5 mM or 7 mM 
      glucose, development to the blastocyst stage was dramatically decreased in 
      comparison to the control group (15.9% vs. 27.8% for 3.5 mM glucose, and 5.4% vs. 
      25.3% for 7 mM glucose). Supplementation of melatonin moderately improved the 
      development of NRF2-attenuated embryos cultured in presence of 0.6 mM glucose. 
      These findings highlight the importance of NRF2 in early embryo development, 
      particularly in embryos cultured under metabolically stressful conditions.
CI  - (c) 2024. The Author(s).
FAU - Glanzner, Werner Giehl
AU  - Glanzner WG
AD  - Department of Animal Science, McGill University, 21111, Lakeshore Road, Sainte 
      Anne de Bellevue, QC, H9X 3V9, Canada. werner.glanzner@mcgill.ca.
FAU - da Silva Sousa, Leticia Rabello
AU  - da Silva Sousa LR
AD  - Veterinary Medicine Department, College of Animal Science and Food Engineering, 
      University of Sao Paulo (USP), Pirassununga, SP, Brazil.
FAU - Gutierrez, Karina
AU  - Gutierrez K
AD  - Department of Animal Science, McGill University, 21111, Lakeshore Road, Sainte 
      Anne de Bellevue, QC, H9X 3V9, Canada.
FAU - de Macedo, Mariana Priotto
AU  - de Macedo MP
AD  - Department of Animal Science, McGill University, 21111, Lakeshore Road, Sainte 
      Anne de Bellevue, QC, H9X 3V9, Canada.
FAU - Currin, Luke
AU  - Currin L
AD  - Department of Animal Science, McGill University, 21111, Lakeshore Road, Sainte 
      Anne de Bellevue, QC, H9X 3V9, Canada.
FAU - Perecin, Felipe
AU  - Perecin F
AD  - Veterinary Medicine Department, College of Animal Science and Food Engineering, 
      University of Sao Paulo (USP), Pirassununga, SP, Brazil.
FAU - Bordignon, Vilceu
AU  - Bordignon V
AD  - Department of Animal Science, McGill University, 21111, Lakeshore Road, Sainte 
      Anne de Bellevue, QC, H9X 3V9, Canada. vilceu.bordignon@mcgill.ca.
LA  - eng
PT  - Journal Article
DEP - 20240205
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (NF-E2-Related Factor 2)
RN  - IY9XDZ35W2 (Glucose)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Swine
MH  - Animals
MH  - *Embryonic Development/genetics
MH  - *NF-E2-Related Factor 2/genetics/metabolism
MH  - Blastocyst/metabolism
MH  - Glucose/metabolism
MH  - Stress, Physiological
MH  - RNA, Messenger/metabolism
MH  - Embryo Culture Techniques
PMC - PMC10844622
COIS- The authors declare no competing interests.
EDAT- 2024/02/06 00:42
MHDA- 2024/02/07 06:43
PMCR- 2024/02/05
CRDT- 2024/02/05 23:36
PHST- 2023/09/16 00:00 [received]
PHST- 2024/01/31 00:00 [accepted]
PHST- 2024/02/07 06:43 [medline]
PHST- 2024/02/06 00:42 [pubmed]
PHST- 2024/02/05 23:36 [entrez]
PHST- 2024/02/05 00:00 [pmc-release]
AID - 10.1038/s41598-024-53480-8 [pii]
AID - 53480 [pii]
AID - 10.1038/s41598-024-53480-8 [doi]
PST - epublish
SO  - Sci Rep. 2024 Feb 5;14(1):2973. doi: 10.1038/s41598-024-53480-8.