PMID- 38319409
OWN - NLM
STAT- MEDLINE
DCOM- 20240207
LR  - 20240207
IS  - 1557-1904 (Electronic)
IS  - 1557-1890 (Linking)
VI  - 19
IP  - 1
DP  - 2024 Feb 6
TI  - Heat Shock Protein 22 Attenuates Nerve Injury-induced Neuropathic Pain Via 
      Improving Mitochondrial Biogenesis and Reducing Oxidative Stress Mediated By 
      Spinal AMPK/PGC-1alpha Pathway in Male Rats.
PG  - 5
LID - 10.1007/s11481-024-10100-6 [doi]
AB  - Heat shock protein 22 (hsp22) plays a significant role in mitochondrial 
      biogenesis and redox balance. Moreover, it's well accepted that the impairment of 
      mitochondrial biogenesis and redox imbalance contributes to the progress of 
      neuropathic pain. However, there is no available evidence indicating that hsp22 
      can ameliorate mechanical allodynia and thermal hyperalgesia, sustain 
      mitochondrial biogenesis and redox balance in rats with neuropathic pain. In this 
      study, pain behavioral test, western blotting, immunofluorescence staining, 
      quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, and 
      Dihydroethidium staining are applied to confirm the role of hsp22 in a male rat 
      model of spared nerve injury (SNI). Our results indicate that hsp22 was 
      significantly decreased in spinal neurons post SNI. Moreover, it was found that 
      intrathecal injection (i.t.) with recombinant heat shock protein 22 protein 
      (rhsp22) ameliorated mechanical allodynia and thermal hyperalgesia, facilitated 
      nuclear respiratory factor 1 (NRF1)/ mitochondrial transcription factor A 
      (TFAM)-dependent mitochondrial biogenesis, decreased the level of reactive oxygen 
      species (ROS), and suppressed oxidative stress via activation of spinal adenosine 
      5'monophosphate-activated protein kinase (AMPK)/ peroxisome proliferative 
      activated receptor gamma coactivator 1alpha (PGC-1alpha) pathway in male rats with SNI. 
      Furthermore, it was also demonstrated that AMPK antagonist (compound C, CC) or 
      PGC-1alpha siRNA reversed the improved mechanical allodynia and thermal hyperalgesia, 
      mitochondrial biogenesis, oxidative stress, and the decreased ROS induced by 
      rhsp22 in male rats with SNI. These results revealed that hsp22 alleviated 
      mechanical allodynia and thermal hyperalgesia, improved the impairment of 
      NRF1/TFAM-dependent mitochondrial biogenesis, down-regulated the level of ROS, 
      and mitigated oxidative stress through stimulating the spinal AMPK/PGC-1alpha pathway 
      in male rats with SNI.
CI  - (c) 2024. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Zhang, Longqing
AU  - Zhang L
AD  - Department of Anesthesiology, Hubei Key Laboratory of Geriatric Anesthesia and 
      Perioperative Brain Health, and Wuhan Clinical Research Center for Geriatric 
      Anesthesia, Tongji Hospital, Tongji Medical College, Huazhong University of 
      Science and Technology, Wuhan, Hubei Province, China.
FAU - Liu, Lin
AU  - Liu L
AD  - Department of Anesthesiology, Hubei Key Laboratory of Geriatric Anesthesia and 
      Perioperative Brain Health, and Wuhan Clinical Research Center for Geriatric 
      Anesthesia, Tongji Hospital, Tongji Medical College, Huazhong University of 
      Science and Technology, Wuhan, Hubei Province, China.
FAU - Li, Danyang
AU  - Li D
AD  - Department of Anesthesiology, Hubei Key Laboratory of Geriatric Anesthesia and 
      Perioperative Brain Health, and Wuhan Clinical Research Center for Geriatric 
      Anesthesia, Tongji Hospital, Tongji Medical College, Huazhong University of 
      Science and Technology, Wuhan, Hubei Province, China.
FAU - Wu, Jiayi
AU  - Wu J
AD  - Department of Anesthesiology, Hubei Key Laboratory of Geriatric Anesthesia and 
      Perioperative Brain Health, and Wuhan Clinical Research Center for Geriatric 
      Anesthesia, Tongji Hospital, Tongji Medical College, Huazhong University of 
      Science and Technology, Wuhan, Hubei Province, China.
FAU - Gao, Shaojie
AU  - Gao S
AD  - Department of Anesthesiology, Hubei Key Laboratory of Geriatric Anesthesia and 
      Perioperative Brain Health, and Wuhan Clinical Research Center for Geriatric 
      Anesthesia, Tongji Hospital, Tongji Medical College, Huazhong University of 
      Science and Technology, Wuhan, Hubei Province, China.
FAU - Song, Fanhe
AU  - Song F
AD  - Department of Anesthesiology, Hubei Key Laboratory of Geriatric Anesthesia and 
      Perioperative Brain Health, and Wuhan Clinical Research Center for Geriatric 
      Anesthesia, Tongji Hospital, Tongji Medical College, Huazhong University of 
      Science and Technology, Wuhan, Hubei Province, China.
FAU - Zhou, Yaqun
AU  - Zhou Y
AD  - Department of Anesthesiology, Hubei Key Laboratory of Geriatric Anesthesia and 
      Perioperative Brain Health, and Wuhan Clinical Research Center for Geriatric 
      Anesthesia, Tongji Hospital, Tongji Medical College, Huazhong University of 
      Science and Technology, Wuhan, Hubei Province, China.
FAU - Liu, Daiqiang
AU  - Liu D
AD  - Department of Anesthesiology, Hubei Key Laboratory of Geriatric Anesthesia and 
      Perioperative Brain Health, and Wuhan Clinical Research Center for Geriatric 
      Anesthesia, Tongji Hospital, Tongji Medical College, Huazhong University of 
      Science and Technology, Wuhan, Hubei Province, China. dqliu2017@hotmail.com.
FAU - Mei, Wei
AU  - Mei W
AD  - Department of Anesthesiology, Hubei Key Laboratory of Geriatric Anesthesia and 
      Perioperative Brain Health, and Wuhan Clinical Research Center for Geriatric 
      Anesthesia, Tongji Hospital, Tongji Medical College, Huazhong University of 
      Science and Technology, Wuhan, Hubei Province, China. wmei@hust.edu.cn.
LA  - eng
GR  - 82001198/National Natural Science Foundation of China/
GR  - 82101310/National Natural Science Foundation of China/
GR  - 82271291 and 82071556/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20240206
PL  - United States
TA  - J Neuroimmune Pharmacol
JT  - Journal of neuroimmune pharmacology : the official journal of the Society on 
      NeuroImmune Pharmacology
JID - 101256586
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (HspB8 protein, rat)
RN  - 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
SB  - IM
MH  - Animals
MH  - Male
MH  - Rats
MH  - AMP-Activated Protein Kinases/metabolism
MH  - *Heat-Shock Proteins/metabolism
MH  - *Hyperalgesia/drug therapy
MH  - *Neuralgia
MH  - Organelle Biogenesis
MH  - Oxidation-Reduction
MH  - Oxidative Stress
MH  - Reactive Oxygen Species/metabolism
MH  - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha/metabolism
OTO - NOTNLM
OT  - Mitochondrial biogenesis
OT  - Mitochondrial dysfunction
OT  - Neuropathic pain
OT  - Oxidative stress
OT  - ROS
OT  - hsp22
EDAT- 2024/02/06 13:43
MHDA- 2024/02/07 06:42
CRDT- 2024/02/06 11:07
PHST- 2023/07/25 00:00 [received]
PHST- 2023/11/14 00:00 [accepted]
PHST- 2024/02/07 06:42 [medline]
PHST- 2024/02/06 13:43 [pubmed]
PHST- 2024/02/06 11:07 [entrez]
AID - 10.1007/s11481-024-10100-6 [pii]
AID - 10.1007/s11481-024-10100-6 [doi]
PST - epublish
SO  - J Neuroimmune Pharmacol. 2024 Feb 6;19(1):5. doi: 10.1007/s11481-024-10100-6.