PMID- 38320714
OWN - NLM
STAT- MEDLINE
DCOM- 20240301
LR  - 20240301
IS  - 1873-2976 (Electronic)
IS  - 0960-8524 (Linking)
VI  - 396
DP  - 2024 Mar
TI  - Rapid anaerobic culture and reaction kinetic study of anammox bacteria on 
      microfluidic chip.
PG  - 130422
LID - S0960-8524(24)00125-1 [pii]
LID - 10.1016/j.biortech.2024.130422 [doi]
AB  - Anammox bacteria are being increasingly investigated as part of an emerging 
      nitrogen removal technology. However, due to the difficulty in culturing, current 
      understanding of their behavior is limited. In this study, anaerobic microfluidic 
      chips were used to study anammox bacteria, showing great advantages over 
      reactors. On-chip fluorescence in situ hybridization (FISH) showed the relative 
      abundance of free form anammox bacteria increased by 56.1 % after one week's 
      culture, an increase that is three times higher than that of bioreactor (17.1 %). 
      For granular form cultures, the nitrogen removal load reached 
      2.34  approximately  2.51 kg-N/(m(3).d), which was also substantially higher than the 
      bioreactor ( approximately 1.22 kg-N/(m(3).d)). Furthermore, studying the kinetics of nitrite 
      inhibition of granular sludge with different particle sizes (100-900 mum) showed 
      that the maximum ammonia load and the nitrite semi-saturation coefficient 
      noticeably decreased for smaller particle sizes. These results illustrate the 
      usefulness of the microfluidic method for in-depth understanding anammox process 
      and its implementation.
CI  - Copyright (c) 2024. Published by Elsevier Ltd.
FAU - Li, Bing
AU  - Li B
AD  - School of Energy and Environmental Engineering, University of Science and 
      Technology Beijing, Beijing 100083, China.
FAU - Mao, Siyuan
AU  - Mao S
AD  - School of Energy and Environmental Engineering, University of Science and 
      Technology Beijing, Beijing 100083, China.
FAU - Zhang, Chi
AU  - Zhang C
AD  - School of Environment, Tsinghua University, Beijing 100084, China.
FAU - Xu, Tiansi
AU  - Xu T
AD  - School of Environment, Tsinghua University, Beijing 100084, China.
FAU - Ma, Xueyan
AU  - Ma X
AD  - School of Environment, Tsinghua University, Beijing 100084, China.
FAU - Lin, Hai
AU  - Lin H
AD  - School of Energy and Environmental Engineering, University of Science and 
      Technology Beijing, Beijing 100083, China.
FAU - Yin, Huabing
AU  - Yin H
AD  - James Watt School of Engineering, University of Glasgow, Glasgow G12 8LT, UK. 
      Electronic address: huabing.yin@glasgow.ac.uk.
FAU - Qiu, Yong
AU  - Qiu Y
AD  - School of Environment, Tsinghua University, Beijing 100084, China. Electronic 
      address: qiuyong@tsinghua.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20240205
PL  - England
TA  - Bioresour Technol
JT  - Bioresource technology
JID - 9889523
RN  - 0 (Nitrites)
RN  - 7664-41-7 (Ammonia)
RN  - N762921K75 (Nitrogen)
RN  - 0 (Sewage)
SB  - IM
MH  - Anaerobiosis
MH  - In Situ Hybridization, Fluorescence
MH  - *Nitrites
MH  - *Anaerobic Ammonia Oxidation
MH  - Microfluidics
MH  - Bacteria/genetics
MH  - Ammonia
MH  - Bioreactors/microbiology
MH  - Oxidation-Reduction
MH  - Nitrogen
MH  - Sewage/microbiology
MH  - Bacteria, Anaerobic
OTO - NOTNLM
OT  - Anammox
OT  - Granular sludge
OT  - Kinetics
OT  - Microfluidic chip
OT  - Nitrite inhibition
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2024/02/07 00:42
MHDA- 2024/03/01 06:44
CRDT- 2024/02/06 19:19
PHST- 2023/12/09 00:00 [received]
PHST- 2024/02/02 00:00 [revised]
PHST- 2024/02/03 00:00 [accepted]
PHST- 2024/03/01 06:44 [medline]
PHST- 2024/02/07 00:42 [pubmed]
PHST- 2024/02/06 19:19 [entrez]
AID - S0960-8524(24)00125-1 [pii]
AID - 10.1016/j.biortech.2024.130422 [doi]
PST - ppublish
SO  - Bioresour Technol. 2024 Mar;396:130422. doi: 10.1016/j.biortech.2024.130422. Epub 
      2024 Feb 5.