PMID- 38321082
OWN - NLM
STAT- MEDLINE
DCOM- 20240208
LR  - 20240210
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 14
IP  - 1
DP  - 2024 Feb 6
TI  - Development of novel polymer haemoglobin based particles as an antioxidant, 
      antibacterial and an oxygen carrier agents.
PG  - 3031
LID - 10.1038/s41598-024-53548-5 [doi]
LID - 3031
AB  - This innovative work aims to develop highly biocompatible and degradable 
      nanoparticles by encapsulating haemoglobin (Hb) within poly-epsilon-caprolactone for 
      novel biomedical applications. We used a modified double emulsion solvent 
      evaporation method to fabricate the particles. A Scanning electron microscope 
      (SEM) characterized them for surface morphology. Fourier Transform Infrared 
      Spectroscopy (FTIR) and Ultraviolet-visible spectroscopies (UV-visible) 
      elucidated preserved chemical and biological structure of encapsulated 
      haemoglobin. The airproof equilibrium apparatus obtained the oxygen-carrying 
      capacity and P(50) values. The DPPH assay assessed free radical scavenging 
      potential. The antibacterial properties were observed using four different 
      bacterial strains by disk diffusion method. The MTT assay investigates the 
      cytotoxic effects on mouse fibroblast cultured cell lines (L-929). The MTT assay 
      showed that nanoparticles have no toxicity over large concentrations. The 
      well-preserved structure of Hb within particles, no toxicity, high oxygen 
      affinity, P(50) value, and IC50 values open the area of new research, which may 
      be used as artificial oxygen carriers, antioxidant, and antibacterial agents, 
      potential therapeutic agents as well as drug carrier particles to treat the 
      cancerous cells. The novelty of this work is the antioxidant and antibacterial 
      properties of developed nanoparticles are not been reported yet. Results showed 
      that the prepared particles have strong antioxidant and antibacterial potential.
CI  - (c) 2024. The Author(s).
FAU - Majid, Muhammad Abdul
AU  - Majid MA
AD  - Biophotonics Imaging Techniques Laboratory, Institute of Physics, The Islamia 
      University of Bahawalpur, Bahawalpur, Pakistan.
FAU - Ullah, Hafeez
AU  - Ullah H
AD  - Biophotonics Imaging Techniques Laboratory, Institute of Physics, The Islamia 
      University of Bahawalpur, Bahawalpur, Pakistan. hafeezullah@iub.edu.pk.
FAU - Alshehri, Ali Mohammad
AU  - Alshehri AM
AD  - Department of Physics, King Khalid University, P.O. Box 9004, 61413, Abha, Saudi 
      Arabia.
FAU - Tabassum, Rukhsana
AU  - Tabassum R
AD  - Institute of Chemistry, The Islamia University of Bahawalpur, Bahawalpur, 
      Pakistan.
FAU - Aleem, Abdul
AU  - Aleem A
AD  - Biophotonics Imaging Techniques Laboratory, Institute of Physics, The Islamia 
      University of Bahawalpur, Bahawalpur, Pakistan.
FAU - Khan, Asad Ur Rehman
AU  - Khan AUR
AD  - Biophotonics Imaging Techniques Laboratory, Institute of Physics, The Islamia 
      University of Bahawalpur, Bahawalpur, Pakistan.
FAU - Batool, Zahida
AU  - Batool Z
AD  - Biophotonics Imaging Techniques Laboratory, Institute of Physics, The Islamia 
      University of Bahawalpur, Bahawalpur, Pakistan.
FAU - Nazir, Aalia
AU  - Nazir A
AD  - Biophotonics Imaging Techniques Laboratory, Institute of Physics, The Islamia 
      University of Bahawalpur, Bahawalpur, Pakistan.
FAU - Bibi, Ismat
AU  - Bibi I
AD  - Institute of Chemistry, The Islamia University of Bahawalpur, Bahawalpur, 
      Pakistan.
LA  - eng
GR  - KKU-IFP2-H-20/ministry of education in Kingdom of Saudi Arabia/
PT  - Journal Article
DEP - 20240206
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antioxidants)
RN  - S88TT14065 (Oxygen)
RN  - 0 (Polymers)
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Hemoglobins)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Antioxidants/pharmacology
MH  - Oxygen
MH  - Polymers
MH  - *Metal Nanoparticles/chemistry
MH  - Anti-Bacterial Agents/pharmacology
MH  - Hemoglobins
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Plant Extracts/chemistry
PMC - PMC10847508
COIS- The authors declare no competing interests.
EDAT- 2024/02/07 00:42
MHDA- 2024/02/08 06:42
PMCR- 2024/02/06
CRDT- 2024/02/06 23:20
PHST- 2023/10/16 00:00 [received]
PHST- 2024/02/01 00:00 [accepted]
PHST- 2024/02/08 06:42 [medline]
PHST- 2024/02/07 00:42 [pubmed]
PHST- 2024/02/06 23:20 [entrez]
PHST- 2024/02/06 00:00 [pmc-release]
AID - 10.1038/s41598-024-53548-5 [pii]
AID - 53548 [pii]
AID - 10.1038/s41598-024-53548-5 [doi]
PST - epublish
SO  - Sci Rep. 2024 Feb 6;14(1):3031. doi: 10.1038/s41598-024-53548-5.