PMID- 38321345
OWN - NLM
STAT- Publisher
LR  - 20240206
IS  - 1573-5168 (Electronic)
IS  - 0920-1742 (Linking)
DP  - 2024 Feb 7
TI  - Addition of alpha-ketoglutaric acid (AKG) reduces deamination in Chinese perch 
      (Siniperca chuatsi) fed with fermented soybean meal as a substitute for fishmeal.
LID - 10.1007/s10695-024-01312-x [doi]
AB  - To alleviate amino acid imbalances in fermented soybean meal as a replacement for 
      fishmeal feeds, this study evaluated the effects of adding lysine (Lys), 
      methionine (Met), and alpha-ketoglutaric acid (AKG) to fermented soybean meals for 
      Chinese perch. Chinese perch (34 +/- 3 g) were fed five diets for 66 days (fishmeal 
      as the protein source of the basal diet [FM]; fermented soybean meal as a 
      substitute for 30% fishmeal in the soybean meal diet [FSM]; addition of 
      crystalline Lys and Met [AA]; addition of alpha-ketoglutaric acid [AKG]; and 
      simultaneous addition of crystalline Lys, Met, and AKG [BA] to the soybean meal 
      diet). At the end of the feeding trial, the FSM group had the highest feeding 
      rate and the lowest weight gain rate among all the groups. The FM group had the 
      highest protein retention and the lowest feed efficiency among the groups. The 
      mRNA transcription level of genes related to the AMP-activating protein (AMPK) 
      signaling pathway and amino acid response (AAR) signaling pathway (lkb1, atf4, 
      and gcn2) were highest in the AA group (P < 0.05) but lower in the AKG and BA 
      groups. In the AKG group, the mRNA transcription level of the gluconeogenesis 
      pathway-related gene (pepck and g6pase) was significantly higher than that in the 
      other four groups, but the mRNA transcription level of genes related to amino 
      acid catabolism (gdh and ampd) was lower. Among all the groups, the FSM group had 
      the lowest mRNA transcription level of genes associated with the mammalian target 
      of rapamycin (mTOR) signaling pathway (mtor and s6k). These findings imply that 
      the feeding rate of Chinese perch in the fermented soybean meal group was the 
      highest, but the protein retention was the lowest, while the addition of Lys, 
      Met, and AKG improved protein retention. In conclusion, the addition of AKG to 
      fermented soybean meal as a fishmeal substitute reduced amino acid deamination, 
      enhanced gluconeogenesis, and increased protein deposition, which contributed to 
      the growth of Chinese perch, alleviated amino acid imbalances, and improved the 
      feed utilization of Chinese perch.
CI  - (c) 2024. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Zhang, Yufei
AU  - Zhang Y
AD  - College of Fisheries, Chinese Perch Research Center, Engineering Research Center 
      of Green Development for Conventional Aquatic Biological Industry in the Yangtze 
      River Economic Belt, Ministry of Education, Huazhong Agricultural University, 
      No.1, Shizishan Street, Hongshan District, Wuhan, 430070, Hubei Province, 
      People's Republic of China.
FAU - Hu, Jiacheng
AU  - Hu J
AD  - College of Fisheries, Chinese Perch Research Center, Engineering Research Center 
      of Green Development for Conventional Aquatic Biological Industry in the Yangtze 
      River Economic Belt, Ministry of Education, Huazhong Agricultural University, 
      No.1, Shizishan Street, Hongshan District, Wuhan, 430070, Hubei Province, 
      People's Republic of China.
FAU - Lu, Peisong
AU  - Lu P
AD  - College of Fisheries, Chinese Perch Research Center, Engineering Research Center 
      of Green Development for Conventional Aquatic Biological Industry in the Yangtze 
      River Economic Belt, Ministry of Education, Huazhong Agricultural University, 
      No.1, Shizishan Street, Hongshan District, Wuhan, 430070, Hubei Province, 
      People's Republic of China.
FAU - Yang, Ru
AU  - Yang R
AD  - College of Fisheries, Chinese Perch Research Center, Engineering Research Center 
      of Green Development for Conventional Aquatic Biological Industry in the Yangtze 
      River Economic Belt, Ministry of Education, Huazhong Agricultural University, 
      No.1, Shizishan Street, Hongshan District, Wuhan, 430070, Hubei Province, 
      People's Republic of China.
FAU - Liang, Xu-Fang
AU  - Liang XF
AD  - College of Fisheries, Chinese Perch Research Center, Engineering Research Center 
      of Green Development for Conventional Aquatic Biological Industry in the Yangtze 
      River Economic Belt, Ministry of Education, Huazhong Agricultural University, 
      No.1, Shizishan Street, Hongshan District, Wuhan, 430070, Hubei Province, 
      People's Republic of China.
FAU - Liu, Liwei
AU  - Liu L
AD  - College of Fisheries, Chinese Perch Research Center, Engineering Research Center 
      of Green Development for Conventional Aquatic Biological Industry in the Yangtze 
      River Economic Belt, Ministry of Education, Huazhong Agricultural University, 
      No.1, Shizishan Street, Hongshan District, Wuhan, 430070, Hubei Province, 
      People's Republic of China. liuliwei@mail.hzau.edu.cn.
AD  - State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life 
      Sciences, Hunan Normal University, Changsha, 410081, Hunan, People's Republic of 
      China. liuliwei@mail.hzau.edu.cn.
LA  - eng
GR  - 3180 2318/National Natural Science Foundation of China/
GR  - 3180 2318/National Natural Science Foundation of China/
GR  - 3180 2318/National Natural Science Foundation of China/
GR  - 3180 2318/National Natural Science Foundation of China/
GR  - 3180 2318/National Natural Science Foundation of China/
GR  - 3180 2318/National Natural Science Foundation of China/
GR  - 2019KF013/the State Key Laboratory of Developmental Biology of Freshwater Fish/
GR  - 2019KF013/the State Key Laboratory of Developmental Biology of Freshwater Fish/
GR  - 2019KF013/the State Key Laboratory of Developmental Biology of Freshwater Fish/
GR  - 2019KF013/the State Key Laboratory of Developmental Biology of Freshwater Fish/
GR  - 2019KF013/the State Key Laboratory of Developmental Biology of Freshwater Fish/
GR  - 2019KF013/the State Key Laboratory of Developmental Biology of Freshwater Fish/
GR  - 2662021SCPY005/Fundamental Research Funds for the Central Universities/
GR  - 2662021SCPY005/Fundamental Research Funds for the Central Universities/
GR  - 2662021SCPY005/Fundamental Research Funds for the Central Universities/
GR  - 2662021SCPY005/Fundamental Research Funds for the Central Universities/
GR  - 2662021SCPY005/Fundamental Research Funds for the Central Universities/
GR  - 2662021SCPY005/Fundamental Research Funds for the Central Universities/
PT  - Journal Article
DEP - 20240207
PL  - Netherlands
TA  - Fish Physiol Biochem
JT  - Fish physiology and biochemistry
JID - 100955049
SB  - IM
OTO - NOTNLM
OT  - Amino acid homeostasis
OT  - Amino acid metabolism
OT  - Chinese perch (Siniperca chuatsi)
OT  - Deamination
OT  - Gluconeogenesis
OT  - alpha-ketoglutaric acid
EDAT- 2024/02/07 00:42
MHDA- 2024/02/07 00:42
CRDT- 2024/02/06 23:33
PHST- 2023/09/21 00:00 [received]
PHST- 2024/01/28 00:00 [accepted]
PHST- 2024/02/07 00:42 [medline]
PHST- 2024/02/07 00:42 [pubmed]
PHST- 2024/02/06 23:33 [entrez]
AID - 10.1007/s10695-024-01312-x [pii]
AID - 10.1007/s10695-024-01312-x [doi]
PST - aheadofprint
SO  - Fish Physiol Biochem. 2024 Feb 7. doi: 10.1007/s10695-024-01312-x.