PMID- 38333754
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240210
IS  - 2008-3866 (Print)
IS  - 2008-3874 (Electronic)
IS  - 2008-3866 (Linking)
VI  - 27
IP  - 3
DP  - 2024
TI  - Therapeutic potential of HUC-MSC-exos primed with IFN-gamma against LPS-induced acute 
      lung injury.
PG  - 375-382
LID - 10.22038/IJBMS.2023.74372.16156 [doi]
AB  - OBJECTIVES: Human umbilical cord mesenchymal stem cells (HUC-MSCs) are 
      pluripotent stem cells with anti-inflammatory and immunomodulatory properties 
      used in the treatment of acute lung injury (ALI). However, the treatment of ALI 
      using exosomes derived from HUC-MSCs (HUC-MSC-exos) primed with interferon-gamma 
      (IFN-gamma-exos) has not been described. This study investigated the effects of 
      IFN-gamma-exos on ALI. MATERIALS AND METHODS: IFN-gamma primed and unprimed HUC-MSC-exos 
      (IFN-gamma-exos and CON-exos, respectively) were extracted, identified, and traced. 
      A549 cells and mice subjected to lipopolysaccharide (LPS)-induced inflammation 
      were treated with IFN-gamma-exos or CON-exos. Viability; interleukin (IL)-1beta, IL-6, 
      tumor necrosis factor (TNF)-alpha, and reactive oxygen species (ROS) levels; NF-kappaB 
      p65, and NLRP3 expression and histology and lung injury scores were measured in 
      cell, supernatant or lung tissue. RESULTS: Indoleamine 2,3-dioxygenase (IDO) mRNA 
      expression was elevated in HUC-MSCs primed with 5 ng/mL IFN-gamma (P<0.001), and 
      IFN-gamma-exos and CON-exos were successfully extracted. LPS-induced inflammation 
      resulted in decreased cell viability in A549 cells, and increased IL-1beta, IL-6, 
      TNF-alpha and ROS levels and NF-kappaB p65 and NLRP3 expression in A549 cells and 
      mice(P<0.05 to P<0.001). Treatment with IFN-gamma-exos and CON-exos increased cell 
      viability and decreased the concentrations of IL-1beta, and ROS, expression of NF-kappaB 
      p65 and NLRP3, and the lung injury score, and these effects were more obvious for 
      IFN-gamma-exos(P<0.05 to P<0.001). CONCLUSION: IFN-gamma-exos reduced oxidative stress 
      and inflammatory responses in LPS-induced A549 cells and mice. The result 
      demonstrated the therapeutic potential of IFN-gamma-exos in LPS-induced ALI.
FAU - Wang, Chun
AU  - Wang C
AD  - Kunming Medical University, Kunming, China.
AD  - Department of Emergency Intensive Care Unit, Second Affiliated Hospital of 
      Kunming Medical University, Kunming, Yunnan, 650000, China.
FAU - Jiang, Chen
AU  - Jiang C
AD  - Kunming Medical University, Kunming, China.
FAU - Yang, Yiran
AU  - Yang Y
AD  - Kunming Medical University, Kunming, China.
FAU - Xi, Cheng
AU  - Xi C
AD  - Department of Gastrointestinal Surgery, First Affiliated Hospital of Kunming 
      Medical University, Kunming, Yunnan, 650000, China.
FAU - Yin, Yunxiang
AU  - Yin Y
AD  - Department of Emergency Intensive Care Unit, Second Affiliated Hospital of 
      Kunming Medical University, Kunming, Yunnan, 650000, China.
FAU - Wu, Haiying
AU  - Wu H
AD  - Department of Emergency, First Affiliated Hospital of Kunming Medical University, 
      Kunming, Yunnan, 650000, China.
FAU - Qian, Chuanyun
AU  - Qian C
AD  - Department of Emergency, First Affiliated Hospital of Kunming Medical University, 
      Kunming, Yunnan, 650000, China.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Basic Med Sci
JT  - Iranian journal of basic medical sciences
JID - 101517966
PMC - PMC10849211
OTO - NOTNLM
OT  - Acute lung injury
OT  - Exosomes
OT  - Inflammation
OT  - Interferon gamma
OT  - Mesenchymal stem cells
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2024/02/09 06:43
MHDA- 2024/02/09 06:44
PMCR- 2024/01/01
CRDT- 2024/02/09 04:09
PHST- 2023/08/15 00:00 [received]
PHST- 2023/10/30 00:00 [accepted]
PHST- 2024/02/09 06:44 [medline]
PHST- 2024/02/09 06:43 [pubmed]
PHST- 2024/02/09 04:09 [entrez]
PHST- 2024/01/01 00:00 [pmc-release]
AID - 10.22038/IJBMS.2023.74372.16156 [doi]
PST - ppublish
SO  - Iran J Basic Med Sci. 2024;27(3):375-382. doi: 10.22038/IJBMS.2023.74372.16156.