PMID- 38335699
OWN - NLM
STAT- MEDLINE
DCOM- 20240226
LR  - 20240226
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 160
DP  - 2024 Apr
TI  - Carbon dots combined with phytosphingosine inhibit acid-induced demineralization 
      of hydroxyapatite in vitro.
PG  - 105911
LID - S0003-9969(24)00032-3 [pii]
LID - 10.1016/j.archoralbio.2024.105911 [doi]
AB  - OBJECTIVES: To study the effects of carbon dots (CDs), in combination with 
      phytosphingosine (PHS), against acid-induced demineralization of hydroxyapatite 
      in vitro. METHODS: CDs were generated from citric acid and urea by microwave 
      heating. Transmission electron microscope (TEM), FT-IR, and fluorescence 
      intensity were used to characterize the CDs. A hydroxyapatite (HAp) model was 
      used to investigate the protective effects of CDs, PHS, and their combinations 
      with and without a salivary pellicle against acid-induced demineralization in 
      vitro. Ca(2+) release as a parameter to evaluate the inhibition of 
      demineralization was measured by capillary electrophoresis. The interactions 
      between CDs, PHS, and HAp discs were investigated using a fluorescence detector. 
      RESULTS: Uniform-sized CDs were synthesized, showing typical optical 
      characteristics. CDs exhibited no inhibition of acid-induced demineralization in 
      vitro, in contrast to PHS. Notably, a pre-coating of CDs increased the protective 
      effects of PHS against acid-induced demineralization, which was not disturbed by 
      the presence of a salivary pellicle and Tween 20. Scanning electron microscope 
      (SEM) confirmed the binding and layers formed of both CDs and PHS to the HAp 
      surfaces. Based on fluorescence spectra CDs binding to HAp seemed to be dependent 
      on Ca(2+) and PO(4)(3-) interactions. CONCLUSIONS: CDs combined with PHS showed 
      protective effects against acid-induced demineralization of HAp discs in vitro.
CI  - Copyright (c) 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Fu, Cuicui
AU  - Fu C
AD  - Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), 
      University of Amsterdam (UvA) and Vrije Universiteit Amsterdam (VU), Amsterdam 
      1081LA, the Netherlands. Electronic address: c.fu@acta.nl.
FAU - Brand, Henk S
AU  - Brand HS
AD  - Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), 
      University of Amsterdam (UvA) and Vrije Universiteit Amsterdam (VU), Amsterdam 
      1081LA, the Netherlands.
FAU - Nazmi, Kamran
AU  - Nazmi K
AD  - Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), 
      University of Amsterdam (UvA) and Vrije Universiteit Amsterdam (VU), Amsterdam 
      1081LA, the Netherlands.
FAU - Werner, Arie
AU  - Werner A
AD  - Department of Dental Materials Science, Academic Centre for Dentistry Amsterdam 
      (ACTA), University of Amsterdam (UvA) and Vrije Universiteit Amsterdam (VU), 
      Amsterdam 1081LA, the Netherlands.
FAU - van Splunter, Annina
AU  - van Splunter A
AD  - Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), 
      University of Amsterdam (UvA) and Vrije Universiteit Amsterdam (VU), Amsterdam 
      1081LA, the Netherlands.
FAU - Bikker, Floris J
AU  - Bikker FJ
AD  - Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), 
      University of Amsterdam (UvA) and Vrije Universiteit Amsterdam (VU), Amsterdam 
      1081LA, the Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20240205
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 91D9GV0Z28 (Durapatite)
RN  - GIN46U9Q2Q (phytosphingosine)
RN  - 7440-44-0 (Carbon)
RN  - NGZ37HRE42 (Sphingosine)
SB  - IM
MH  - Humans
MH  - *Durapatite/pharmacology
MH  - Carbon/pharmacology
MH  - Spectroscopy, Fourier Transform Infrared
MH  - *Tooth Demineralization
MH  - Sphingosine/*analogs & derivatives
OTO - NOTNLM
OT  - Acid-induced demineralization
OT  - Carbon dots
OT  - Dental erosion
OT  - Hydroxyapatite
OT  - Phytosphingosine
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2024/02/10 13:18
MHDA- 2024/02/26 06:44
CRDT- 2024/02/09 18:03
PHST- 2023/11/08 00:00 [received]
PHST- 2024/01/29 00:00 [revised]
PHST- 2024/02/01 00:00 [accepted]
PHST- 2024/02/26 06:44 [medline]
PHST- 2024/02/10 13:18 [pubmed]
PHST- 2024/02/09 18:03 [entrez]
AID - S0003-9969(24)00032-3 [pii]
AID - 10.1016/j.archoralbio.2024.105911 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2024 Apr;160:105911. doi: 10.1016/j.archoralbio.2024.105911. Epub 
      2024 Feb 5.