PMID- 38337023
OWN - NLM
STAT- MEDLINE
DCOM- 20240214
LR  - 20240214
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 14
IP  - 1
DP  - 2024 Feb 9
TI  - Investigating the genetic makeup of the major histocompatibility complex (MHC) in 
      the United Arab Emirates population through next-generation sequencing.
PG  - 3392
LID - 10.1038/s41598-024-53986-1 [doi]
LID - 3392
AB  - The Human leukocyte antigen (HLA) molecules are central to immune response and 
      have associations with the phenotypes of various diseases and induced drug 
      toxicity. Further, the role of HLA molecules in presenting antigens significantly 
      affects the transplantation outcome. The objective of this study was to examine 
      the extent of the diversity of HLA alleles in the population of the United Arab 
      Emirates (UAE) using Next-Generation Sequencing methodologies and encompassing a 
      larger cohort of individuals. A cohort of 570 unrelated healthy citizens of the 
      UAE volunteered to provide samples for Whole Genome Sequencing and Whole Exome 
      Sequencing. The definition of the HLA alleles was achieved through the 
      application of the bioinformatics tools, HLA-LA and xHLA. Subsequently, the 
      findings from this study were compared with other local and international 
      datasets. A broad range of HLA alleles in the UAE population, of which some were 
      previously unreported, was identified. A comparison with other populations 
      confirmed the current population's unique intertwined genetic heritage while 
      highlighting similarities with populations from the Middle East region. Some 
      disease-associated HLA alleles were detected at a frequency of > 5%, such as 
      HLA-B*51:01, HLA-DRB1*03:01, HLA-DRB1*15:01, and HLA-DQB1*02:01. The increase in 
      allele homozygosity, especially for HLA class I genes, was identified in samples 
      with a higher level of genome-wide homozygosity. This highlights a possible 
      effect of consanguinity on the HLA homozygosity. The HLA allele distribution in 
      the UAE population showcases a unique profile, underscoring the need for tailored 
      databases for traditional activities such as unrelated transplant matching and 
      for newer initiatives in precision medicine based on specific populations. This 
      research is part of a concerted effort to improve the knowledge base, 
      particularly in the fields of transplant medicine and investigating disease 
      associations as well as in understanding human migration patterns within the 
      Arabian Peninsula and surrounding regions.
CI  - (c) 2024. The Author(s).
FAU - Marzouka, Nour Al Dain
AU  - Marzouka NAD
AD  - Center for Biotechnology, Khalifa University of Science and Technology, Abu 
      Dhabi, United Arab Emirates.
FAU - Alnaqbi, Halima
AU  - Alnaqbi H
AD  - Center for Biotechnology, Khalifa University of Science and Technology, Abu 
      Dhabi, United Arab Emirates.
FAU - Al-Aamri, Amira
AU  - Al-Aamri A
AD  - Center for Biotechnology, Khalifa University of Science and Technology, Abu 
      Dhabi, United Arab Emirates.
FAU - Tay, Guan
AU  - Tay G
AD  - Division of Psychiatry, Faculty of Health and Medical Sciences, Medical School, 
      The University of Western Australia, Crawley, WA, Australia.
AD  - School of Medical and Health Sciences, Edith Cowan University, Joondalup, WA, 
      Australia.
FAU - Alsafar, Habiba
AU  - Alsafar H
AD  - Center for Biotechnology, Khalifa University of Science and Technology, Abu 
      Dhabi, United Arab Emirates. Habiba.alsafar@ku.ac.ae.
AD  - College of Medicine and Health Sciences, Khalifa University of Science and 
      Technology, Abu Dhabi, United Arab Emirates. Habiba.alsafar@ku.ac.ae.
AD  - Department of Biomedical Engineering, Khalifa University of Science and 
      Technology, Abu Dhabi, United Arab Emirates. Habiba.alsafar@ku.ac.ae.
LA  - eng
PT  - Journal Article
DEP - 20240209
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (HLA-DRB1 Chains)
SB  - IM
MH  - Humans
MH  - United Arab Emirates
MH  - Gene Frequency
MH  - *Histocompatibility Antigens Class I/genetics
MH  - *Histocompatibility Antigens Class II/genetics
MH  - Major Histocompatibility Complex/genetics
MH  - High-Throughput Nucleotide Sequencing
MH  - Haplotypes
MH  - Alleles
MH  - HLA-DRB1 Chains/genetics
PMC - PMC10858242
OTO - NOTNLM
OT  - Human leukocyte antigen (HLA)
OT  - Major histocompatibility complex (MHC)
OT  - Next generation sequencing (NGS)
OT  - United Arab Emirates (UAE)
COIS- The authors declare no competing interests.
EDAT- 2024/02/10 10:47
MHDA- 2024/02/10 22:54
PMCR- 2024/02/09
CRDT- 2024/02/10 00:00
PHST- 2023/11/26 00:00 [received]
PHST- 2024/02/07 00:00 [accepted]
PHST- 2024/02/10 22:54 [medline]
PHST- 2024/02/10 10:47 [pubmed]
PHST- 2024/02/10 00:00 [entrez]
PHST- 2024/02/09 00:00 [pmc-release]
AID - 10.1038/s41598-024-53986-1 [pii]
AID - 53986 [pii]
AID - 10.1038/s41598-024-53986-1 [doi]
PST - epublish
SO  - Sci Rep. 2024 Feb 9;14(1):3392. doi: 10.1038/s41598-024-53986-1.