PMID- 38338233
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240212
IS  - 2227-9032 (Print)
IS  - 2227-9032 (Electronic)
IS  - 2227-9032 (Linking)
VI  - 12
IP  - 3
DP  - 2024 Jan 30
TI  - The Impact of 8 Weeks of Combined Physical Exercise Training on SIRT3 and mTOR in 
      Lymphocytes, and on Lipid Peroxidation.
LID - 10.3390/healthcare12030350 [doi]
LID - 350
AB  - The sirtuins (SIRT) protein family and the mechanistic/mammalian target of 
      rapamycin (mTOR) are intracellular molecules that have been involved in the 
      regulation of several biological processes, as well as in various aging-related 
      processes. This pilot study, in small scale, aimed to analyze the effects of an 
      8-week physical exercise program on SIRT3 and mTOR levels in lymphocytes, as well 
      as on lipid peroxidation in middle aged and older men. A total of 9 participants 
      aged between 56 and 73 years were enrolled in an 8-week physical exercise program 
      comprising cardiovascular and high-intensity interval training. The program 
      involved three sessions per week, each lasting 45-60 min, conducted on 
      non-consecutive days. Tests were conducted before and after the experimental 
      period (pre- and post-training). Assessments included a vertical jump, 20 m 
      velocity, ball throwing, and an aerobic capacity test. Lipid peroxidation (MDA) 
      was measured in plasma as an oxidative stress biomarker. Additionally, sirtuin 3 
      (SIRT3/beta-actin) and mTOR (mTOR/beta-actin) levels were measured in isolated 
      lymphocytes extracted from venous blood. Following the exercise training period, 
      our results demonstrated a significant improvement in aerobic capacity 
      (pre-training: 615.4 +/- 45.3 m; post-training: 687.2 +/- 34.6 m; t = -2.521; p = 
      0.012) and 20 m velocity (pre-training: 4.6 +/- 0.5 s; post-training: 4.3 +/- 0.3 s; 
      t = -2.023; p = 0.04). Concerning blood variables, there was a significant 
      decrease in mTOR levels (pre-training: 0.857 +/- 0.593; post-training: 0.214 +/- 
      0.097; t = -2.547; p = 0.011), while no changes were observed in SIRT3 
      (pre-training: 0.608 +/- 0.404; post-training: 0.516 +/- 0.390; t = 0.533; p = 0.594) 
      and MDA (pre-training: 8420 +/- 4615; post-training: 8800 +/- 3163; t = -0.533; p = 
      0.594). The notable reduction in mTOR levels in lymphocytes following the 8-week 
      physical exercise program suggests a potential role of exercise in modulating 
      immune cell dynamics, particularly in middle-aged and older individuals. 
      Furthermore, the exercise regimen resulted in improvements in physical function, 
      including enhanced aerobic capacity and walking velocity.
FAU - Soares, Jorge Pinto
AU  - Soares JP
AUID- ORCID: 0000-0002-1447-2872
AD  - Research Centre in Sports Sciences, Health, and Human Development (CIDESD), 
      5001-801 Vila Real, Portugal.
AD  - Department of Sport of Science Exercise and Health, School of Life and 
      Environmental Sciences (ECVA), University of Tras-os-Montes and Alto Douro 
      (UTAD), 5000-801 Vila Real, Portugal.
FAU - Cardoso, Ricardo
AU  - Cardoso R
AD  - Centre for the Research and Technology of Agro-Environmental and Biological 
      Sciences (CITAB), University of Tras-os-Montes and Alto Douro (UTAD), 5000-801 
      Vila Real, Portugal.
FAU - Almeida, Vanessa
AU  - Almeida V
AUID- ORCID: 0000-0003-4627-4464
AD  - Centre for the Research and Technology of Agro-Environmental and Biological 
      Sciences (CITAB), University of Tras-os-Montes and Alto Douro (UTAD), 5000-801 
      Vila Real, Portugal.
FAU - Pereira, Ana Fatima
AU  - Pereira AF
AUID- ORCID: 0000-0003-4581-422X
AD  - Polytechnic Institute of Setubal (IPS), 2910-761 Setubal, Portugal.
FAU - Silva, Amelia M
AU  - Silva AM
AUID- ORCID: 0000-0002-7524-9914
AD  - Centre for the Research and Technology of Agro-Environmental and Biological 
      Sciences (CITAB), University of Tras-os-Montes and Alto Douro (UTAD), 5000-801 
      Vila Real, Portugal.
AD  - Department of Biology and Environment, University of Tras-os-Montes and Alto 
      Douro (UTAD), 5000-801 Vila Real, Portugal.
FAU - Mota, Maria Paula
AU  - Mota MP
AD  - Research Centre in Sports Sciences, Health, and Human Development (CIDESD), 
      5001-801 Vila Real, Portugal.
AD  - Department of Sport of Science Exercise and Health, School of Life and 
      Environmental Sciences (ECVA), University of Tras-os-Montes and Alto Douro 
      (UTAD), 5000-801 Vila Real, Portugal.
LA  - eng
GR  - UIDB/04033/2020 (CITAB)/Fundacao para a Ciencia e Tecnologia/
PT  - Journal Article
DEP - 20240130
PL  - Switzerland
TA  - Healthcare (Basel)
JT  - Healthcare (Basel, Switzerland)
JID - 101666525
PMC - PMC10855888
OTO - NOTNLM
OT  - SIRT
OT  - aging
OT  - lymphocytes
OT  - mTOR
OT  - oxidative stress
OT  - physical exercise
COIS- The authors declare no conflicts of interest.
EDAT- 2024/02/10 10:45
MHDA- 2024/02/10 10:46
PMCR- 2024/01/30
CRDT- 2024/02/10 01:07
PHST- 2023/10/03 00:00 [received]
PHST- 2024/01/15 00:00 [revised]
PHST- 2024/01/17 00:00 [accepted]
PHST- 2024/02/10 10:46 [medline]
PHST- 2024/02/10 10:45 [pubmed]
PHST- 2024/02/10 01:07 [entrez]
PHST- 2024/01/30 00:00 [pmc-release]
AID - healthcare12030350 [pii]
AID - healthcare-12-00350 [pii]
AID - 10.3390/healthcare12030350 [doi]
PST - epublish
SO  - Healthcare (Basel). 2024 Jan 30;12(3):350. doi: 10.3390/healthcare12030350.