PMID- 38339001
OWN - NLM
STAT- MEDLINE
DCOM- 20240214
LR  - 20240228
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 25
IP  - 3
DP  - 2024 Jan 31
TI  - Inhibiting Neutrophil Extracellular Traps Protects against Ultraviolet B-Induced 
      Skin Damage: Effects of Hochu-ekki-to and DNase I.
LID - 10.3390/ijms25031723 [doi]
LID - 1723
AB  - UV-B radiation induces sunburn, and neutrophils are pivotal in this inflammation. 
      In this study, we examined the potential involvement of neutrophil extracellular 
      traps (NETs) in ultraviolet B (UVB)-induced skin inflammation, correlating the 
      skin inflammation-mitigating effects of Hochu-ekki-to on UV-B irradiation and 
      NETs. To elucidate NET distribution in the dorsal skin, male ICR mice, exposed to 
      UVB irradiation, were immunohistologically analyzed to detect citrullinated 
      histone H3 (citH3) and peptidylarginine deiminase 4 (PAD4). Reactive oxygen 
      species (ROS) production in the bloodstream was analyzed. To establish the 
      involvement of NET-released DNA in this inflammatory response, mice were UV-B 
      irradiated following the intraperitoneal administration of DNase I. In vitro 
      experiments were performed to scrutinize the impact of Hochu-ekki-to on 
      A23187-induced NETs in neutrophil-like HL-60 cells. UV-B irradiation induced 
      dorsal skin inflammation, coinciding with a significant increase in citH3 and 
      PAD4 expression. Administration of DNase I attenuated UV-B-induced skin 
      inflammation, whereas Hochu-ekki-to administration considerably suppressed the 
      inflammation, correlating with diminished levels of citH3 and PAD4 in the dorsal 
      skin. UV-B irradiation conspicuously augmented ROS and hydrogen peroxide 
      (H(2)O(2)) production in the blood. Hochu-ekki-to significantly inhibited ROS and 
      H(2)O(2) generation. In vitro experiments demonstrated that Hochu-ekki-to notably 
      inhibited A23187-induced NETs in differentiated neutrophil-like cells. Hence, 
      NETs have been implicated in UV-B-induced skin inflammation, and their inhibition 
      reduces cutaneous inflammation. Additionally, Hochu-ekki-to mitigated skin 
      inflammation by impeding neutrophil infiltration and NETs in the dorsal skin of 
      mice.
FAU - Inaba, Issei
AU  - Inaba I
AD  - Department of Biochemistry, Faculty of Pharmaceutical Sciences, Suzuka University 
      of Medical Science, 3500-3, Minamitamagaki, Suzuka 513-8670, Japan.
FAU - Hiramoto, Keiichi
AU  - Hiramoto K
AD  - Department of Biochemistry, Faculty of Pharmaceutical Sciences, Suzuka University 
      of Medical Science, 3500-3, Minamitamagaki, Suzuka 513-8670, Japan.
FAU - Yamate, Yurika
AU  - Yamate Y
AD  - Department of Biochemistry, Faculty of Pharmaceutical Sciences, Suzuka University 
      of Medical Science, 3500-3, Minamitamagaki, Suzuka 513-8670, Japan.
FAU - Morita, Akihiro
AU  - Morita A
AD  - Department of Biochemistry, Faculty of Pharmaceutical Sciences, Suzuka University 
      of Medical Science, 3500-3, Minamitamagaki, Suzuka 513-8670, Japan.
FAU - Tsutsumi, Tomonari
AU  - Tsutsumi T
AD  - Department of Biochemistry, Faculty of Pharmaceutical Sciences, Suzuka University 
      of Medical Science, 3500-3, Minamitamagaki, Suzuka 513-8670, Japan.
FAU - Yasuda, Hiroyuki
AU  - Yasuda H
AD  - Division of Pathological Sciences, Department of Pharmacology and Experimental 
      Therapeutics, Kyoto Pharmaceutical University, Misasagi 5, Yamashina, Kyoto 
      607-8414, Japan.
FAU - Sato, Eisuke F
AU  - Sato EF
AD  - Department of Biochemistry, Faculty of Pharmaceutical Sciences, Suzuka University 
      of Medical Science, 3500-3, Minamitamagaki, Suzuka 513-8670, Japan.
LA  - eng
GR  - JP22K11790/Japan Society for the Promotion of Science/
PT  - Journal Article
DEP - 20240131
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (bu-zhong-yi-qi-tang)
RN  - 37H9VM9WZL (Calcimycin)
RN  - EC 3.1.21.1 (Deoxyribonuclease I)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Histones)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 3.5.3.15 (Protein-Arginine Deiminases)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Animals
MH  - Male
MH  - Mice
MH  - Calcimycin/pharmacology
MH  - *Deoxyribonuclease I/pharmacology/metabolism
MH  - *Drugs, Chinese Herbal
MH  - *Extracellular Traps/drug effects/radiation effects
MH  - Histones/metabolism
MH  - Hydrogen Peroxide/metabolism
MH  - Inflammation/metabolism
MH  - Mice, Inbred ICR
MH  - Neutrophils/metabolism
MH  - Protein-Arginine Deiminases/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - *Ultraviolet Rays/adverse effects
PMC - PMC10855064
OTO - NOTNLM
OT  - Hochu-ekki-to
OT  - NETosis
OT  - UV-B irradiation
OT  - histone citrullination
OT  - inflammation
OT  - neutrophil extracellular trap
COIS- The authors declare no conflicts of interest.
EDAT- 2024/02/10 10:44
MHDA- 2024/02/10 14:47
PMCR- 2024/01/31
CRDT- 2024/02/10 01:12
PHST- 2023/12/28 00:00 [received]
PHST- 2024/01/26 00:00 [revised]
PHST- 2024/01/29 00:00 [accepted]
PHST- 2024/02/10 14:47 [medline]
PHST- 2024/02/10 10:44 [pubmed]
PHST- 2024/02/10 01:12 [entrez]
PHST- 2024/01/31 00:00 [pmc-release]
AID - ijms25031723 [pii]
AID - ijms-25-01723 [pii]
AID - 10.3390/ijms25031723 [doi]
PST - epublish
SO  - Int J Mol Sci. 2024 Jan 31;25(3):1723. doi: 10.3390/ijms25031723.