PMID- 38339050
OWN - NLM
STAT- MEDLINE
DCOM- 20240214
LR  - 20240214
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 25
IP  - 3
DP  - 2024 Feb 1
TI  - Label-Free Liquid Chromatography-Mass Spectrometry Quantitation of Relative N- 
      and O-Glycan Concentrations in Human Milk in Japan.
LID - 10.3390/ijms25031772 [doi]
LID - 1772
AB  - Human milk is abundant in carbohydrates and includes human milk oligosaccharides 
      (HMOs) and N/O-glycans conjugated to proteins. HMO compositions and 
      concentrations vary in individuals according to the maternal secretor status 
      based on the fucosyltransferase 2 genotype; however, the profile of N/O-glycans 
      remains uninvestigated because of the analytical complexity. Herein, we applied a 
      label-free chromatography-mass spectrometry (LC-MS) technique to elucidate the 
      variation in the composition and concentration of N/O-glycans in human milk. We 
      used label-free LC-MS to relatively quantify 16 N-glycans and 12 O-glycans in 200 
      samples of Japanese human milk (1-2 months postpartum) and applied high 
      performance anion exchange chromatography with pulsed amperometric detection to 
      absolutely quantify the concentrations of 11 representative HMOs. Cluster 
      analysis of the quantitative data revealed that O-glycans and several HMOs were 
      classified according to the presence or absence of fucose linked to galactose 
      while N-glycans were classified into a different group from O-glycans and HMOs. 
      O-glycans and HMOs with fucose linked to galactose were more abundant in human 
      milk from secretor mothers than from nonsecretor mothers. Thus, secretor status 
      influenced the composition and concentration of HMOs and O-glycans but not those 
      of N-glycans in human milk.
FAU - Yamaguchi, Toshiyuki
AU  - Yamaguchi T
AUID- ORCID: 0009-0008-5674-7054
AD  - Milk Science Research Institute, Megmilk Snow Brand Co., Ltd., 1-1-2 Minamidai, 
      Kawagoe-shi 350-1165, Saitama, Japan.
FAU - Fukudome, Hirofumi
AU  - Fukudome H
AD  - Milk Science Research Institute, Megmilk Snow Brand Co., Ltd., 1-1-2 Minamidai, 
      Kawagoe-shi 350-1165, Saitama, Japan.
FAU - Higuchi, Junichi
AU  - Higuchi J
AD  - Milk Science Research Institute, Megmilk Snow Brand Co., Ltd., 1-1-2 Minamidai, 
      Kawagoe-shi 350-1165, Saitama, Japan.
FAU - Takahashi, Tomoki
AU  - Takahashi T
AUID- ORCID: 0000-0002-1039-7393
AD  - Department of Research and Development, Bean Stalk Snow Co., Ltd., 1-1-2 
      Minamidai, Kawagoe-shi 350-1165, Saitama, Japan.
FAU - Tsujimori, Yuta
AU  - Tsujimori Y
AD  - Department of Research and Development, Bean Stalk Snow Co., Ltd., 1-1-2 
      Minamidai, Kawagoe-shi 350-1165, Saitama, Japan.
FAU - Ueno, Hiroshi M
AU  - Ueno HM
AUID- ORCID: 0000-0002-8293-9163
AD  - Milk Science Research Institute, Megmilk Snow Brand Co., Ltd., 1-1-2 Minamidai, 
      Kawagoe-shi 350-1165, Saitama, Japan.
AD  - Department of Research and Development, Bean Stalk Snow Co., Ltd., 1-1-2 
      Minamidai, Kawagoe-shi 350-1165, Saitama, Japan.
FAU - Toba, Yasuhiro
AU  - Toba Y
AD  - Department of Research and Development, Bean Stalk Snow Co., Ltd., 1-1-2 
      Minamidai, Kawagoe-shi 350-1165, Saitama, Japan.
FAU - Sakai, Fumihiko
AU  - Sakai F
AUID- ORCID: 0000-0002-5379-4823
AD  - Milk Science Research Institute, Megmilk Snow Brand Co., Ltd., 1-1-2 Minamidai, 
      Kawagoe-shi 350-1165, Saitama, Japan.
LA  - eng
PT  - Journal Article
DEP - 20240201
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 28RYY2IV3F (Fucose)
RN  - X2RN3Q8DNE (Galactose)
RN  - 0 (Polysaccharides)
RN  - 0 (Oligosaccharides)
SB  - IM
MH  - Female
MH  - Humans
MH  - *Milk, Human/chemistry
MH  - Japan
MH  - *Fucose/analysis
MH  - Galactose
MH  - Liquid Chromatography-Mass Spectrometry
MH  - Polysaccharides/analysis
MH  - Mass Spectrometry
MH  - Oligosaccharides/chemistry
PMC - PMC10855831
OTO - NOTNLM
OT  - N-glycan
OT  - O-glycan
OT  - human milk
OT  - human milk oligosaccharide
OT  - liquid chromatography-mass spectrometry
OT  - secretor status
COIS- Yamaguchi, T., Fukudome, H., Higuchi, J. Ueno, H. M., and Sakai, F. are employees 
      of Megmilk Snow Brand Co., Ltd. Megmilk Snow Brand manufactures and sells dairy 
      foods in Japan and some Asian and Oceanian countries. Takahashi, T., Tsujimori, 
      Y., Ueno, H. M., and Toba, Y. are employees of Bean Stalk Snow Co., Ltd. Bean 
      Stalk Snow sells infant formulas and dietary supplements for infants and pregnant 
      and lactating women in Japan.
EDAT- 2024/02/10 10:51
MHDA- 2024/02/10 22:54
PMCR- 2024/02/01
CRDT- 2024/02/10 01:12
PHST- 2023/12/28 00:00 [received]
PHST- 2024/01/26 00:00 [revised]
PHST- 2024/01/29 00:00 [accepted]
PHST- 2024/02/10 22:54 [medline]
PHST- 2024/02/10 10:51 [pubmed]
PHST- 2024/02/10 01:12 [entrez]
PHST- 2024/02/01 00:00 [pmc-release]
AID - ijms25031772 [pii]
AID - ijms-25-01772 [pii]
AID - 10.3390/ijms25031772 [doi]
PST - epublish
SO  - Int J Mol Sci. 2024 Feb 1;25(3):1772. doi: 10.3390/ijms25031772.