PMID- 38342297
OWN - NLM
STAT- MEDLINE
DCOM- 20240308
LR  - 20240308
IS  - 1880-3865 (Electronic)
IS  - 1349-0079 (Linking)
VI  - 66
IP  - 1
DP  - 2024 Mar
TI  - Role of macrophages in trigeminal ganglia in ectopic orofacial pain associated 
      with pulpitis.
PG  - 145-150
LID - S1349-0079(24)00014-8 [pii]
LID - 10.1016/j.job.2024.02.001 [doi]
AB  - OBJECTIVES: This study aimed to elucidate the role of macrophages in the 
      trigeminal ganglia (TG) in developing pulpitis-associated ectopic orofacial pain. 
      METHODS: Rats underwent maxillary pulp exposure, and Fluoro-Gold (FG) was 
      administered in the ipsilateral whisker pad (WP). Head withdrawal threshold (HWT) 
      upon mechanical stimulation of the WP was recorded, and liposomal clodronate 
      clophosome-A (LCCA; macrophage depletion agent) was administered to the TG at 
      three and four days after pulp exposure. Immunohistochemically, TG sections were 
      stained with anti-Iba1 (a macrophage marker) and anti-Nav1.7 antibodies. RESULTS: 
      Pulp exposure decreased HWT and increased the number of Iba1-IR cells near 
      FG-labelled TG neurons. LCCA inhibited the decrease in HWT and stopped the 
      increase of FG-labelled Nav1.7-IR TG neurons in the pulpitis group. CONCLUSIONS: 
      Activation of macrophages by pulpitis induces the overexpression of Nav1.7 in TG 
      neurons receiving inputs from WP, resulting in pulpitis-induced ectopic facial 
      mechanical allodynia.
CI  - Copyright (c) 2024 Japanese Association for Oral Biology. Published by Elsevier 
      B.V. All rights reserved.
FAU - Sunaga, Miki
AU  - Sunaga M
AD  - Department of Physiology, Nihon University School of Dentistry, Tokyo, Japan.
FAU - Tsuboi, Yoshiyuki
AU  - Tsuboi Y
AD  - Department of Physiology, Nihon University School of Dentistry, Tokyo, Japan. 
      Electronic address: tsuboi.yoshiyuki@nihon-u.ac.jp.
FAU - Kaizu, Akihiro
AU  - Kaizu A
AD  - Department of Physiology, Nihon University School of Dentistry, Tokyo, Japan.
FAU - Shinoda, Masamichi
AU  - Shinoda M
AD  - Department of Physiology, Nihon University School of Dentistry, Tokyo, Japan.
LA  - eng
PT  - Journal Article
DEP - 20240210
PL  - Netherlands
TA  - J Oral Biosci
JT  - Journal of oral biosciences
JID - 101226721
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Pulpitis
MH  - Rats, Sprague-Dawley
MH  - Trigeminal Ganglion
MH  - Facial Pain
MH  - Macrophages
OTO - NOTNLM
OT  - Ectopic pain
OT  - Macrophages
OT  - Nav1.7
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2024/02/12 05:43
MHDA- 2024/03/08 06:43
CRDT- 2024/02/11 19:15
PHST- 2023/11/27 00:00 [received]
PHST- 2024/01/31 00:00 [revised]
PHST- 2024/02/01 00:00 [accepted]
PHST- 2024/03/08 06:43 [medline]
PHST- 2024/02/12 05:43 [pubmed]
PHST- 2024/02/11 19:15 [entrez]
AID - S1349-0079(24)00014-8 [pii]
AID - 10.1016/j.job.2024.02.001 [doi]
PST - ppublish
SO  - J Oral Biosci. 2024 Mar;66(1):145-150. doi: 10.1016/j.job.2024.02.001. Epub 2024 
      Feb 10.