PMID- 38346639
OWN - NLM
STAT- MEDLINE
DCOM- 20240313
LR  - 20240313
IS  - 1873-6424 (Electronic)
IS  - 0269-7491 (Linking)
VI  - 345
DP  - 2024 Mar 15
TI  - Combined toxicity of microplastics and copper on Goniopora columns.
PG  - 123515
LID - S0269-7491(24)00229-X [pii]
LID - 10.1016/j.envpol.2024.123515 [doi]
AB  - As microplastics (MP) become ubiquitous, their interactions with heavy metals 
      threatens the coral ecosystem. This study aimed to assess the combined toxicity 
      of MP and copper (Cu) in the environment of coral. Goniopora columna was exposed 
      to polyethylene microplastics (PE-MP) combined with Cu(2+) at 10, 20, 50, 100, 
      and 300 mug/L for 7 days. Polyp length and adaptability were recorded daily, and 
      coral samples were collected at 1, 3, 5, and 7 days to analyse zooxanthellae 
      density and antioxidant activity. Tissue observations and the analysis of MP and 
      Cu(2+) accumulation were conducted on the 7th day. After 1 day of exposure, PE-MP 
      combined with different concentrations of Cu(2+) significantly decreased polyp 
      length and adaptability compared with PE-MP alone. Simultaneously, a significant 
      increase in malondialdehyde (MDA) content, lead to coral oxidative stress, which 
      was a combined effect with PE-MP. After 3 days of exposure, PE-MP combined with 
      Cu(2+) at >50 mug/L significantly reduced zooxanthellae density, damaging the 
      coral's symbiotic relationship. In antioxidant enzyme activity, superoxide 
      dismutase (SOD) activity decreased significantly after 1 day of exposure. After 3 
      days of exposure, glutathione peroxidase (GPx) activity significantly increased 
      with Cu(2+) at >20 mug/L. After 5 days of exposure, PE-MP combined with different 
      concentrations of Cu(2+) significantly reduced catalase (CAT), glutathione (GSH), 
      and glutathione transferase (GST) activity, disrupting the antioxidant enzyme 
      system, and acting antagonistically to PE-MP alone. Tissue observations revealed 
      that the PE-MP combined with Cu(2+) at >50 mug/L caused severe mesenteric atrophy, 
      vacuolar, and Cu(2+) accumulation in the coral mesenteric compared with PE-MP 
      alone. The results suggest that combined exposure of PE-MP and copper leads to 
      more severe oxidative stress, disruption antioxidant enzyme system, tissue 
      damage, and Cu(2+) accumulation, resulting in a significant maladaptation of 
      corals to the environment.
CI  - Copyright (c) 2024 Elsevier Ltd. All rights reserved.
FAU - Chen, Ya-Ting
AU  - Chen YT
AD  - Department of Seafood Science, National Kaohsiung University of Science and 
      Technology, Kaohsiung, 81157, Taiwan.
FAU - Ding, De-Sing
AU  - Ding DS
AD  - Department and Graduate Institute of Aquaculture, National Kaohsiung University 
      of Science and Technology, Kaohsiung, 81157, Taiwan.
FAU - Lim, Yee Cheng
AU  - Lim YC
AD  - Department of Marine Environmental Engineering, National Kaohsiung University of 
      Science and Technology, Kaohsiung, 81157, Taiwan.
FAU - Dong, Cheng-Di
AU  - Dong CD
AD  - Department of Marine Environmental Engineering, National Kaohsiung University of 
      Science and Technology, Kaohsiung, 81157, Taiwan.
FAU - Hsieh, Shu-Ling
AU  - Hsieh SL
AD  - Department of Seafood Science, National Kaohsiung University of Science and 
      Technology, Kaohsiung, 81157, Taiwan. Electronic address: slhsieh@nkust.edu.tw.
LA  - eng
PT  - Journal Article
DEP - 20240210
PL  - England
TA  - Environ Pollut
JT  - Environmental pollution (Barking, Essex : 1987)
JID - 8804476
RN  - 789U1901C5 (Copper)
RN  - 0 (Antioxidants)
RN  - 0 (Microplastics)
RN  - 0 (Plastics)
RN  - GAN16C9B8O (Glutathione)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - *Copper/toxicity
MH  - Antioxidants/metabolism
MH  - Microplastics
MH  - Plastics
MH  - Ecosystem
MH  - Oxidative Stress
MH  - Glutathione/metabolism
MH  - Superoxide Dismutase/metabolism
MH  - *Water Pollutants, Chemical/toxicity
OTO - NOTNLM
OT  - Combined toxicity
OT  - Copper
OT  - Goniopora columna
OT  - Histopathology
OT  - Oxidative stress
OT  - Polyethylene microplastics
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2024/02/13 00:42
MHDA- 2024/03/13 06:47
CRDT- 2024/02/12 19:16
PHST- 2023/11/14 00:00 [received]
PHST- 2024/01/21 00:00 [revised]
PHST- 2024/02/05 00:00 [accepted]
PHST- 2024/03/13 06:47 [medline]
PHST- 2024/02/13 00:42 [pubmed]
PHST- 2024/02/12 19:16 [entrez]
AID - S0269-7491(24)00229-X [pii]
AID - 10.1016/j.envpol.2024.123515 [doi]
PST - ppublish
SO  - Environ Pollut. 2024 Mar 15;345:123515. doi: 10.1016/j.envpol.2024.123515. Epub 
      2024 Feb 10.