PMID- 38347910
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240214
IS  - 1178-7007 (Print)
IS  - 1178-7007 (Electronic)
IS  - 1178-7007 (Linking)
VI  - 17
DP  - 2024
TI  - Efficacy of Spleen-and-Stomach-Tonifying, Yin-Fire-Purging, and Yang-Raising 
      Decoction Derived from the Trimethylamine N-Oxide Metabolic Pathway of Intestinal 
      Microbiota on Macrovascular Lesions Caused by Type 2 Diabetes Mellitus.
PG  - 585-596
LID - 10.2147/DMSO.S431435 [doi]
AB  - OBJECTIVE: We aimed to analyze the mechanisms underlying 
      spleen-and-stomach-tonifying, yin-fire-purging, and yang-raising decoction 
      derived from the trimethylamine N-oxide (TMAO) metabolic pathway of intestinal 
      microbiota in the treatment of macrovascular lesions caused by type 2 diabetes 
      mellitus (T2DM). METHODS: Hartley-guinea pigs were randomly divided into 3 
      groups-the blank, model, and intervention groups. The T2DM combined with 
      atherosclerosis guinea pig models were established in the model and intervention 
      groups. After successful modeling, spleen-and-stomach-tonifying, 
      yin-fire-purging, and yang-raising decoction were administered intragastrically 
      to the intervention group, while the same volume of normal saline was 
      administered via gavage to the blank and model groups. After 6 weeks of 
      continuous gavage, guinea pigs were sacrificed in all groups, the colon contents 
      were obtained, and the diversity and structural differences of intestinal 
      microbiota were analyzed via bioinformatics. Serum was collected to detect 
      differences in lipids, TMAO, oxidative stress, and inflammation markers between 
      groups. RESULTS: Compared to the blank group, the species diversity of the 
      intestinal microbiota in the model and intervention groups was significantly 
      reduced. Based on the results of Analysis of Similarities and Multiple Response 
      Permutation Procedure, the microbiota structure of the intervention group was 
      closer to that of the blank group. After modeling, the blood lipid levels of 
      guinea pigs increased significantly, and drug intervention significantly reduced 
      the levels of TC, TG, and LDL-C (P < 0.05). TMAO expression was significantly 
      increased after modeling (P < 0.05), while drug intervention reduced TMAO 
      expression (P < 0.05). Compared to the model group, drug intervention 
      significantly increased the concentrations of SOD while decreasing the 
      concentrations of MDA, ICAM-1, VCAM-1, IL-6, and hs-CRP. CONCLUSION: 
      Spleen-and-stomach-tonifying, yin-fire-purging, and yang-raising decoction can 
      reduce the risk of macrovascular lesions in T2DM, and its mechanism may be 
      associated with its ability to regulate the TMAO metabolic pathway of intestinal 
      microbiota.
CI  - (c) 2024 Yue et al.
FAU - Yue, Yue
AU  - Yue Y
AD  - Department of Endocrinology, The 983 Hospital of the Joint Logistics Force of the 
      Chinese People's Liberation Army, Tianjin, People's Republic of China.
FAU - Cui, Han-Bo
AU  - Cui HB
AD  - Department of Diabetes, Tianjin Academy of Traditional Chinese Medicine 
      Affiliated Hospital, Tianjin, People's Republic of China.
FAU - Chu, Yue-Jie
AU  - Chu YJ
AD  - Department of Diabetes, Tianjin Academy of Traditional Chinese Medicine 
      Affiliated Hospital, Tianjin, People's Republic of China.
FAU - Zheng, Gui-Ling
AU  - Zheng GL
AD  - Department of Endocrinology, The 983 Hospital of the Joint Logistics Force of the 
      Chinese People's Liberation Army, Tianjin, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20240207
PL  - New Zealand
TA  - Diabetes Metab Syndr Obes
JT  - Diabetes, metabolic syndrome and obesity : targets and therapy
JID - 101515585
PMC - PMC10859761
OTO - NOTNLM
OT  - and yang-raising decoction
OT  - intestinal microbiota
OT  - macrovascular lesions
OT  - spleen-and-stomach-tonifying
OT  - trimethylamine N-oxide
OT  - type 2 diabetes mellitus
OT  - yin-fire-purging
COIS- The authors report no conflicts of interest in this work.
EDAT- 2024/02/13 06:45
MHDA- 2024/02/13 06:46
PMCR- 2024/02/07
CRDT- 2024/02/13 03:34
PHST- 2023/07/19 00:00 [received]
PHST- 2024/01/11 00:00 [accepted]
PHST- 2024/02/13 06:46 [medline]
PHST- 2024/02/13 06:45 [pubmed]
PHST- 2024/02/13 03:34 [entrez]
PHST- 2024/02/07 00:00 [pmc-release]
AID - 431435 [pii]
AID - 10.2147/DMSO.S431435 [doi]
PST - epublish
SO  - Diabetes Metab Syndr Obes. 2024 Feb 7;17:585-596. doi: 10.2147/DMSO.S431435. 
      eCollection 2024.