PMID- 38352213
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240215
IS  - 1976-1457 (Print)
IS  - 2005-6168 (Electronic)
IS  - 1976-1457 (Linking)
VI  - 18
IP  - 1
DP  - 2024 Feb
TI  - Effects of an in vitro vitamin D treatment on the inflammatory responses in 
      visceral adipose tissue from Ldlr(-/-) mice.
PG  - 19-32
LID - 10.4162/nrp.2024.18.1.19 [doi]
AB  - BACKGROUND/OBJECTIVES: Atherosclerosis is associated with increased inflammation 
      in the visceral adipose tissue (VAT). Vitamin D has been reported to modulate the 
      inflammatory responses of stromal vascular cells (SVCs) and adipocytes in adipose 
      tissue, but the role of vitamin D in atherosclerosis biology is unclear. This 
      study examined the effects of in vitro 1,25-dihydroxyvitamin D(3) 
      (1,25[OH](2)D(3)) treatment on the inflammatory responses of SVCs and adipocytes 
      from atherosclerotic mice. MATERIALS/METHODS: C57BL/6J (B6) mice were divided 
      randomly into 2 groups and fed a 10% kcal fat control diet (control group, CON) 
      or 41% kcal fat, 0.21% cholesterol (high fat + cholesterol, HFC) diet (obese 
      group, OB), and B6.129S7-Ldlr(tm1Her)/J (Ldlr(-/-)) mice were fed a HFC diet 
      (obese with atherosclerosis group, OBA) for 16 weeks. SVCs and adipocytes 
      isolated from VAT were pre-incubated with 1,25(OH)(2)D(3) for 24 h and stimulated 
      with lipopolysaccarides for the next 24 h. Proinflammatory cytokine production by 
      adipocytes and SVCs, the immune cell population in SVCs, and the expression of 
      the genes involved in the inflammatory signaling pathway in SVCs were determined. 
      RESULTS: The numbers of total macrophages and SVCs per mouse were higher in OB 
      and OBA groups than the CON group. The in vitro 1,25(OH)(2)D(3) treatment 
      significantly reduced macrophages/SVCs (%) in the OBA group. Consistent with this 
      change, the production of interleukin-6 and monocyte chemoattractant protein 1 
      (MCP-1) by SVCs from the OBA group was decreased by 1,25(OH)(2)D(3) treatment. 
      The 1,25(OH)(2)D(3) treatment significantly reduced the toll-like receptor 4 and 
      dual-specificity protein phosphatase 1 (also known as mitogen-activated protein 
      kinase phosphatase 1) mRNA levels in SVCs and MCP-1 production by adipocytes from 
      all 3 groups. CONCLUSIONS: These findings suggest that vitamin D can attribute to 
      the inhibition of the inflammatory response in VAT from atherosclerotic mice by 
      reducing proinflammatory cytokine production.
CI  - (c)2024 The Korean Nutrition Society and the Korean Society of Community Nutrition.
FAU - Kwon, Deok Hoon
AU  - Kwon DH
AUID- ORCID: 0000-0002-8647-6776
AD  - Department of Food and Nutrition, College of Human Ecology, Seoul National 
      University, Seoul 08826, Korea.
FAU - Hwang, Jungwon
AU  - Hwang J
AUID- ORCID: 0000-0001-5545-3291
AD  - Department of Food and Nutrition, College of Human Ecology, Seoul National 
      University, Seoul 08826, Korea.
FAU - You, Hyeyoung
AU  - You H
AUID- ORCID: 0000-0002-9833-6412
AD  - Department of Food and Nutrition, College of Human Ecology, Seoul National 
      University, Seoul 08826, Korea.
FAU - Kim, Na Young
AU  - Kim NY
AUID- ORCID: 0000-0001-8725-8677
AD  - Department of Food and Nutrition, College of Human Ecology, Seoul National 
      University, Seoul 08826, Korea.
FAU - Lee, Ga Young
AU  - Lee GY
AUID- ORCID: 0000-0002-3906-0671
AD  - Department of Food and Nutrition, College of Human Ecology, Seoul National 
      University, Seoul 08826, Korea.
FAU - Han, Sung Nim
AU  - Han SN
AUID- ORCID: 0000-0003-0647-2992
AD  - Department of Food and Nutrition, College of Human Ecology, Seoul National 
      University, Seoul 08826, Korea.
AD  - Research Institute of Human Ecology, College of Human Ecology, Seoul National 
      University, Seoul 08826, Korea.
LA  - eng
PT  - Journal Article
DEP - 20231211
PL  - Korea (South)
TA  - Nutr Res Pract
JT  - Nutrition research and practice
JID - 101311052
PMC - PMC10861343
OTO - NOTNLM
OT  - Atherosclerosis
OT  - adipose tissue
OT  - inflammation
OT  - vitamin D
COIS- Conflict of Interest: The authors declare no potential conflicts of interests.
EDAT- 2024/02/14 06:43
MHDA- 2024/02/14 06:44
PMCR- 2024/02/01
CRDT- 2024/02/14 03:51
PHST- 2023/07/31 00:00 [received]
PHST- 2023/10/26 00:00 [revised]
PHST- 2023/11/16 00:00 [accepted]
PHST- 2024/02/14 06:44 [medline]
PHST- 2024/02/14 06:43 [pubmed]
PHST- 2024/02/14 03:51 [entrez]
PHST- 2024/02/01 00:00 [pmc-release]
AID - 10.4162/nrp.2024.18.1.19 [doi]
PST - ppublish
SO  - Nutr Res Pract. 2024 Feb;18(1):19-32. doi: 10.4162/nrp.2024.18.1.19. Epub 2023 
      Dec 11.