PMID- 38356236
OWN - NLM
STAT- MEDLINE
DCOM- 20240417
LR  - 20240417
IS  - 1522-7278 (Electronic)
IS  - 1520-4081 (Linking)
VI  - 39
IP  - 5
DP  - 2024 May
TI  - Supercritical CO(2) fluid extract from Stellariae Radix ameliorates 
      2,4-dinitrochlorobenzene-induced atopic dermatitis by inhibit M1 macrophages 
      polarization via AMPK activation.
PG  - 3188-3197
LID - 10.1002/tox.24145 [doi]
AB  - Yin chai hu (Radix Stellariae) is a root medicine that is frequently used in 
      Chinese traditional medicine to treat fever and malnutrition. In modern medicine, 
      it has been discovered to have anti-inflammatory, anti-allergic, and anticancer 
      properties. In a previous study, we were able to extract lipids from Stellariae 
      Radix using supercritical CO(2) extraction (SRE), and these sterol lipids 
      accounted for up to 88.29% of the extract. However, the impact of SRE on the 
      development of atopic dermatitis (AD) has not yet been investigated. This study 
      investigates the inhibitory effects of SRE on AD development using a 
      2,4-dinitrochlorobenzene (DNCB)-induced AD mouse model. Treatment with SRE 
      significantly reduced the dermatitis score and histopathological changes compared 
      with the DNCB group. The study found that treatment with SRE resulted in a 
      decrease of pro-inflammatory cytokines TNF-alpha, CXC-10, IL-12, and IL-1beta in skin 
      lesions. Additionally, immunohistochemical analysis revealed that SRE effectively 
      suppressed M1 macrophage infiltration into the AD lesion. Furthermore, the 
      anti-inflammatory effect of SRE was evaluated in LPS + INF-gamma induced bone 
      marrow-derived macrophages (BMDMs) M1 polarization, SRE inhibited the production 
      of TNF-alpha, CXC-10, IL-12, and IL-1beta and decreased the expression of NLRP3. 
      Additionally, SRE was found to increase p-AMPK(T172), but had no effect on total 
      AMPK expression, after administration of the AMPK inhibitor Compound C, the 
      inhibitory effect of SRE on M1 macrophages was partially reversed. The results 
      indicate that SRE has an inhibitory effect on AD, making it a potential 
      therapeutic agent for this atopic disorder.
CI  - (c) 2024 Wiley Periodicals LLC.
FAU - Wu, Wei
AU  - Wu W
AUID- ORCID: 0000-0001-6710-6211
AD  - School of Life Sciences, Ningxia University, Yinchuan, China.
FAU - Song, Le
AU  - Song L
AD  - School of Life Sciences, Ningxia University, Yinchuan, China.
FAU - Wang, Hong
AU  - Wang H
AD  - School of Life Sciences, Ningxia University, Yinchuan, China.
FAU - Feng, Lu
AU  - Feng L
AD  - School of Life Sciences, Ningxia University, Yinchuan, China.
FAU - Li, Zhenkai
AU  - Li Z
AD  - School of Life Sciences, Ningxia University, Yinchuan, China.
FAU - Li, Yanqing
AU  - Li Y
AD  - School of Life Sciences, Ningxia University, Yinchuan, China.
FAU - Li, Le
AU  - Li L
AD  - School of Life Sciences, Ningxia University, Yinchuan, China.
FAU - Peng, Li
AU  - Peng L
AD  - School of Life Sciences, Ningxia University, Yinchuan, China.
LA  - eng
GR  - 2021FRD05024/Central Government Guided Local Special Basic Research Program/
GR  - 2020AAC02013/Ningxia Natural Science Foundation of China/
GR  - 2021BEG02042/Ningxia Provincial Key Research and Development Project/
PT  - Journal Article
DEP - 20240214
PL  - United States
TA  - Environ Toxicol
JT  - Environmental toxicology
JID - 100885357
RN  - 0 (Dinitrochlorobenzene)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - 142M471B3J (Carbon Dioxide)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Cytokines)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 187348-17-0 (Interleukin-12)
RN  - 0 (Lipids)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Dermatitis, Atopic/chemically induced/drug therapy/metabolism
MH  - Dinitrochlorobenzene/toxicity/therapeutic use
MH  - AMP-Activated Protein Kinases
MH  - Carbon Dioxide/toxicity/therapeutic use
MH  - Tumor Necrosis Factor-alpha
MH  - Cytokines/metabolism
MH  - Macrophages/metabolism
MH  - Anti-Inflammatory Agents/therapeutic use
MH  - Interleukin-12/toxicity/therapeutic use
MH  - Lipids
MH  - Mice, Inbred BALB C
MH  - Skin
OTO - NOTNLM
OT  - AMPK activation
OT  - NLRP3
OT  - Stellariae Radix supercritical CO2 fluid extract
OT  - atopic dermatitis
OT  - macrophages polarization
EDAT- 2024/02/15 06:42
MHDA- 2024/04/17 06:42
CRDT- 2024/02/15 01:04
PHST- 2023/12/29 00:00 [revised]
PHST- 2023/10/05 00:00 [received]
PHST- 2024/01/06 00:00 [accepted]
PHST- 2024/04/17 06:42 [medline]
PHST- 2024/02/15 06:42 [pubmed]
PHST- 2024/02/15 01:04 [entrez]
AID - 10.1002/tox.24145 [doi]
PST - ppublish
SO  - Environ Toxicol. 2024 May;39(5):3188-3197. doi: 10.1002/tox.24145. Epub 2024 Feb 
      14.