PMID- 38361581 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240217 IS - 2297-055X (Print) IS - 2297-055X (Electronic) IS - 2297-055X (Linking) VI - 11 DP - 2024 TI - Promising results of a clinical feasibility study: CIRBP as a potential biomarker in pediatric cardiac surgery. PG - 1247472 LID - 10.3389/fcvm.2024.1247472 [doi] LID - 1247472 AB - OBJECTIVE: Cold-inducible RNA binding Protein (CIRBP) has been shown to be a potent inflammatory mediator and could serve as a novel biomarker for inflammation. Systemic inflammatory response syndrome (SIRS) and capillary leak syndrome (CLS) are frequent complications after pediatric cardiac surgery increasing morbidity, therefore early diagnosis and therapy is crucial. As CIRBP serum levels have not been analyzed in a pediatric population, we conducted a clinical feasibility establishing a customized magnetic bead panel analyzing CIRBP in pediatric patients undergoing cardiac surgery. METHODS: A prospective hypothesis generating observational clinical study was conducted at the German Heart Center Berlin during a period of 9 months starting in May 2020 (DRKS00020885, https://drks.de/search/de/trial/DRKS00020885). Serum samples were obtained before the cardiac operation, upon arrival at the pediatric intensive care unit, 6 and 24 h after the operation in patients up to 18 years of age with congenital heart disease (CHD). Customized multiplex magnetic bead-based immunoassay panels were developed to analyze CIRBP, Interleukin-1beta (IL-1beta), Interleukin-6 (IL-6), Interleukin-8 (IL-8), Interleukin-10 (IL-10), Monocyte chemotactic protein 1 (MCP-1), Syndecan-1 (SDC-1), Thrombomodulin (TM), Vascular endothelial growth factor (VEGF-A), Angiopoietin-2 (Ang-2), and Fibroblast growth factor 23 (FGF-23) in 25 microl serum using the Luminex MagPix(R) system. RESULTS: 19 patients representing a broad range of CHD (10 male patients, median age 2 years, 9 female patients, median age 3 years) were included in the feasibility study. CIRBP was detectable in the whole patient cohort. Relative to individual baseline values, CIRBP concentrations increased 6 h after operation and returned to baseline levels over time. IL-6, IL-8, IL-10, and MCP-1 concentrations were significantly increased after operation and except for MCP-1 concentrations stayed upregulated over time. SDC-1, TM, Ang-2, as well as FGF-23 concentrations were also significantly increased, whereas VEGF-A concentration was significantly decreased after surgery. DISCUSSION: Using customized magnetic bead panels, we were able to detect CIRBP in a minimal serum volume (25 microl) in all enrolled patients. To our knowledge this is the first clinical study to assess CIRBP serum concentrations in a pediatric population. CI - (c) 2024 Lucht, Seiler, Herre, Brankova, Fritsche-Guenther, Kirwan, Huscher, Munzfeld, Berger, Photiadis, Tong and Schmitt. FAU - Lucht, Jana AU - Lucht J AD - Department of Congenital Heart Disease/Pediatric Cardiology, Deutsches Herzzentrum der Charite - Medical Heart Center of Charite and German Heart Institute Berlin, Berlin, Germany. AD - Berlin Institute of Health at Charite - Universitatsmedizin Berlin, Berlin, Germany. FAU - Seiler, Raphael AU - Seiler R AD - Department of Congenital Heart Disease/Pediatric Cardiology, Deutsches Herzzentrum der Charite - Medical Heart Center of Charite and German Heart Institute Berlin, Berlin, Germany. FAU - Herre, Alexa Leona AU - Herre AL AD - Department of Congenital Heart Disease/Pediatric Cardiology, Deutsches Herzzentrum der Charite - Medical Heart Center of Charite and German Heart Institute Berlin, Berlin, Germany. FAU - Brankova, Liliya AU - Brankova L AD - Department of Congenital Heart Disease/Pediatric Cardiology, Deutsches Herzzentrum der Charite - Medical Heart Center of Charite and German Heart Institute Berlin, Berlin, Germany. FAU - Fritsche-Guenther, Raphaela AU - Fritsche-Guenther R AD - Metabolomics Platform, Berlin Institute of Health at Charite - Universitatsmedizin Berlin, Berlin, Germany. FAU - Kirwan, Jennifer AU - Kirwan J AD - Metabolomics Platform, Berlin Institute of Health at Charite - Universitatsmedizin Berlin, Berlin, Germany. FAU - Huscher, Dorte AU - Huscher D AD - Institute of Biometry and Clinical Epidemiology, Charite - Universitatsmedizin Berlin, Corporate Member of Freie Universitat Berlin and Humboldt-Universitat zu Berlin, Berlin, Germany. AD - Berlin Institute of Health at Charite - Universitatsmedizin Berlin, Berlin, Germany. FAU - Munzfeld, Hanna AU - Munzfeld H AD - Department of Radiology, Charite - Universitatsmedizin Berlin, Corporate Member of Freie Universitat Berlin and Humboldt-Universitat zu Berlin, Berlin, Germany. FAU - Berger, Felix AU - Berger F AD - Department of Congenital Heart Disease/Pediatric Cardiology, Deutsches Herzzentrum der Charite - Medical Heart Center of Charite and German Heart Institute Berlin, Berlin, Germany. FAU - Photiadis, Joachim AU - Photiadis J AD - Department of Congenital Heart Surgery and Pediatric Heart Surgery, Deutsches Herzzentrum der Charite - Medical Heart Center of Charite and German Heart Institute Berlin, Berlin, Germany. FAU - Tong, Giang AU - Tong G AD - Department of Congenital Heart Disease/Pediatric Cardiology, Deutsches Herzzentrum der Charite - Medical Heart Center of Charite and German Heart Institute Berlin, Berlin, Germany. FAU - Schmitt, Katharina R L AU - Schmitt KRL AD - Department of Congenital Heart Disease/Pediatric Cardiology, Deutsches Herzzentrum der Charite - Medical Heart Center of Charite and German Heart Institute Berlin, Berlin, Germany. LA - eng PT - Journal Article DEP - 20240201 PL - Switzerland TA - Front Cardiovasc Med JT - Frontiers in cardiovascular medicine JID - 101653388 PMC - PMC10867162 OTO - NOTNLM OT - biomarker OT - cold inducible RNA-binding protein (CIRBP) OT - endothelial dysfunction OT - feasibility study OT - inflammation OT - pediatric cardiac surgery OT - pediatric cardiology COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2024/02/16 06:43 MHDA- 2024/02/16 06:44 PMCR- 2024/01/01 CRDT- 2024/02/16 03:45 PHST- 2023/06/26 00:00 [received] PHST- 2024/01/19 00:00 [accepted] PHST- 2024/02/16 06:44 [medline] PHST- 2024/02/16 06:43 [pubmed] PHST- 2024/02/16 03:45 [entrez] PHST- 2024/01/01 00:00 [pmc-release] AID - 10.3389/fcvm.2024.1247472 [doi] PST - epublish SO - Front Cardiovasc Med. 2024 Feb 1;11:1247472. doi: 10.3389/fcvm.2024.1247472. eCollection 2024.