PMID- 38361789
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240217
IS  - 2296-4185 (Print)
IS  - 2296-4185 (Electronic)
IS  - 2296-4185 (Linking)
VI  - 12
DP  - 2024
TI  - In vitro evaluation of the osteogenic and antimicrobial potential of porous 
      wollastonite scaffolds impregnated with ethanolic extracts of propolis.
PG  - 1321466
LID - 10.3389/fbioe.2024.1321466 [doi]
LID - 1321466
AB  - Context: The development of porous devices using materials modified with various 
      natural agents has become a priority for bone healing processes in the oral and 
      maxillofacial field. There must be a balance between the proliferation of 
      eukaryotic and the inhibition of prokaryotic cells to achieve proper bone health. 
      Infections might inhibit the formation of new alveolar bone during bone graft 
      augmentation. Objective: This study aimed to evaluate the in vitro osteogenic 
      behavior of human bone marrow stem cells and assess the antimicrobial response to 
      3D-printed porous scaffolds using propolis-modified wollastonite. Methodology: A 
      fractional factorial design of experiments was used to obtain a 3D printing paste 
      for developing scaffolds with a triply periodic minimal surface (TPMS) gyroid 
      geometry based on wollastonite and modified with an ethanolic propolis extract. 
      The antioxidant activity of the extracts was characterized using free radical 
      scavenging methods (DPPH and ABTS). Cell proliferation and osteogenic potential 
      using Human Bone Marrow Stem Cells (bmMSCs) were assessed at different culture 
      time points up to 28 days. MIC and inhibition zones were studied from single 
      strain cultures, and biofilm formation was evaluated on the scaffolds under 
      co-culture conditions. The mechanical strength of the scaffolds was evaluated. 
      Results: Through statistical design of experiments, a paste suitable for printing 
      scaffolds with the desired geometry was obtained. Propolis extracts modifying the 
      TPMS gyroid scaffolds showed favorable cell proliferation and metabolic activity 
      with osteogenic potential after 21 days. Additionally, propolis exhibited 
      antioxidant activity, which may be related to the antimicrobial effectiveness of 
      the scaffolds against S. aureus and S. epidermidis cultures. The mechanical 
      properties of the scaffolds were not affected by propolis impregnation. 
      Conclusion: These results demonstrate that propolis-impregnated porous 
      wollastonite scaffolds might have the potential to stimulate bone repair in 
      maxillofacial tissue engineering applications.
CI  - Copyright (c) 2024 Moreno Florez, Malagon, Ocampo, Leal-Marin, Ossa, Glasmacher, 
      Garcia and Pelaez-Vargas.
FAU - Moreno Florez, Ana Isabel
AU  - Moreno Florez AI
AD  - Grupo de Materiales Ceramicos y Vitreos, Universidad Nacional de Colombia Sede 
      Medellin, Medellin, Colombia.
FAU - Malagon, Sarita
AU  - Malagon S
AD  - Grupo GIOM, Facultad de Odontologia, Universidad Cooperativa de Colombia, Sede 
      Medellin, Colombia.
FAU - Ocampo, Sebastian
AU  - Ocampo S
AD  - Grupo de Materiales Ceramicos y Vitreos, Universidad Nacional de Colombia Sede 
      Medellin, Medellin, Colombia.
FAU - Leal-Marin, Sara
AU  - Leal-Marin S
AD  - Institute for Multiphase Processes (IMP), Leibniz University Hannover, Garbsen, 
      Germany.
AD  - 4 Lower Saxony Center for Biomedical Engineering, Implant Research and 
      Development, Hannover, Germany.
FAU - Ossa, Edgar Alexander
AU  - Ossa EA
AD  - School of Applied Sciences and Engineering, Universidad Eafit, Medellin, 
      Colombia.
FAU - Glasmacher, Birgit
AU  - Glasmacher B
AD  - Institute for Multiphase Processes (IMP), Leibniz University Hannover, Garbsen, 
      Germany.
AD  - 4 Lower Saxony Center for Biomedical Engineering, Implant Research and 
      Development, Hannover, Germany.
FAU - Garcia, Claudia
AU  - Garcia C
AD  - Grupo de Materiales Ceramicos y Vitreos, Universidad Nacional de Colombia Sede 
      Medellin, Medellin, Colombia.
FAU - Pelaez-Vargas, Alejandro
AU  - Pelaez-Vargas A
AD  - Grupo GIOM, Facultad de Odontologia, Universidad Cooperativa de Colombia, Sede 
      Medellin, Colombia.
LA  - eng
PT  - Journal Article
DEP - 20240201
PL  - Switzerland
TA  - Front Bioeng Biotechnol
JT  - Frontiers in bioengineering and biotechnology
JID - 101632513
PMC - PMC10867276
OTO - NOTNLM
OT  - 3D printing
OT  - antimicrobial activity
OT  - bone tissue engineering
OT  - human bone marrow stem cells
OT  - wollastonite
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2024/02/16 06:42
MHDA- 2024/02/16 06:43
PMCR- 2024/01/01
CRDT- 2024/02/16 03:48
PHST- 2023/10/14 00:00 [received]
PHST- 2024/01/11 00:00 [accepted]
PHST- 2024/02/16 06:43 [medline]
PHST- 2024/02/16 06:42 [pubmed]
PHST- 2024/02/16 03:48 [entrez]
PHST- 2024/01/01 00:00 [pmc-release]
AID - 1321466 [pii]
AID - 10.3389/fbioe.2024.1321466 [doi]
PST - epublish
SO  - Front Bioeng Biotechnol. 2024 Feb 1;12:1321466. doi: 10.3389/fbioe.2024.1321466. 
      eCollection 2024.