PMID- 38362323
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240217
IS  - 2189-4779 (Print)
IS  - 2189-4779 (Electronic)
IS  - 2189-4779 (Linking)
VI  - 20
IP  - Supplemental
DP  - 2023 Mar 21
TI  - Solid-state NMR for the characterization of retinal chromophore and Schiff base 
      in TAT rhodopsin embedded in membranes under weakly acidic conditions.
PG  - e201017
LID - 10.2142/biophysico.bppb-v20.s017 [doi]
LID - e201017
AB  - TAT rhodopsin extracted from the marine bacterium SAR11 HIMB114 has a 
      characteristic Thr-Ala-Thr motif and contains both protonated and deprotonated 
      states of Schiff base at physiological pH conditions due to the low pK(a.) Here, 
      using solid-state NMR spectroscopy, we investigated the (13)C and (15)N NMR 
      signals of retinal in only the protonated state of TAT in the 
      1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine/1-palmitoyl-2-oleoyl-sn-glycero-3-phospho 
      (1'-rac-glycerol) (POPE/POPG) membrane at weakly acidic conditions. In the (13)C 
      NMR spectrum of (13)C retinal-labeled TAT rhodopsin, the isolated 14-(13)C 
      signals of 13-trans/15-anti and 13-cis/15-syn isomers were observed at a ratio of 
      7:3. (15)N retinal protonated Schiff base (RPSB) had a significantly higher 
      magnetic field resonance at 160 ppm. In (15)N RPSB/lambda(max) analysis, the plot of 
      TAT largely deviated from the trend based on the retinylidene-halide model 
      compounds and microbial rhodopsins. Our findings indicate that the RPSB of TAT 
      forms a very weak interaction with the counterion.
CI  - 2023 THE BIOPHYSICAL SOCIETY OF JAPAN.
FAU - Arikawa, Sui
AU  - Arikawa S
AD  - Graduate School of Engineering Science, Yokohama National University, Yokohama, 
      Kanagawa 240-8501, Japan.
FAU - Sugimoto, Teppei
AU  - Sugimoto T
AD  - Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, 
      Nagoya, Aichi 466-8555, Japan.
FAU - Okitsu, Takashi
AU  - Okitsu T
AD  - Faculty of Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan.
AD  - Laboratory of Organic Chemistry for Life Science, Kobe Pharmaceutical University, 
      Kobe, Hyogo 658-8558, Japan.
FAU - Wada, Akimori
AU  - Wada A
AD  - Laboratory of Organic Chemistry for Life Science, Kobe Pharmaceutical University, 
      Kobe, Hyogo 658-8558, Japan.
FAU - Katayama, Kota
AU  - Katayama K
AD  - Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, 
      Nagoya, Aichi 466-8555, Japan.
AD  - OptoBioTechnology Research Center, Nagoya Institute of Technology, Nagoya, Aichi 
      466-8555, Japan.
FAU - Kandori, Hideki
AU  - Kandori H
AD  - Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, 
      Nagoya, Aichi 466-8555, Japan.
AD  - OptoBioTechnology Research Center, Nagoya Institute of Technology, Nagoya, Aichi 
      466-8555, Japan.
FAU - Kawamura, Izuru
AU  - Kawamura I
AD  - Graduate School of Engineering Science, Yokohama National University, Yokohama, 
      Kanagawa 240-8501, Japan.
LA  - eng
PT  - Journal Article
DEP - 20230302
PL  - Japan
TA  - Biophys Physicobiol
JT  - Biophysics and physicobiology
JID - 101675089
PMC - PMC10865839
OTO - NOTNLM
OT  - chemical shift
OT  - hydrogen bond
OT  - microbial rhodopsin
OT  - retinal protonated Schiff base
OT  - solid-state nuclear magnetic resonance
COIS- The authors declare that no competing interests exist.
EDAT- 2024/02/16 06:42
MHDA- 2024/02/16 06:43
PMCR- 2023/03/02
CRDT- 2024/02/16 03:57
PHST- 2023/01/10 00:00 [received]
PHST- 2023/03/01 00:00 [accepted]
PHST- 2024/02/16 06:43 [medline]
PHST- 2024/02/16 06:42 [pubmed]
PHST- 2024/02/16 03:57 [entrez]
PHST- 2023/03/02 00:00 [pmc-release]
AID - JST.JSTAGE/biophysico/e201017 [pii]
AID - 10.2142/biophysico.bppb-v20.s017 [doi]
PST - epublish
SO  - Biophys Physicobiol. 2023 Mar 2;20(Supplemental):e201017. doi: 
      10.2142/biophysico.bppb-v20.s017. eCollection 2023 Mar 21.