PMID- 38368769
OWN - NLM
STAT- MEDLINE
DCOM- 20240417
LR  - 20240506
IS  - 1095-9157 (Electronic)
IS  - 0896-8411 (Linking)
VI  - 144
DP  - 2024 Apr
TI  - The enigma of sclera-specific autoimmunity in scleritis.
PG  - 103178
LID - S0896-8411(24)00012-X [pii]
LID - 10.1016/j.jaut.2024.103178 [doi]
AB  - Scleritis is a severe and painful ophthalmic disorder, in which a pathogenic role 
      for collagen-directed autoimmunity was repeatedly suggested. We evaluated the 
      presence of sclera-specific antibodies in a large cohort of patients with 
      non-infectious scleritis. Therefore, we prospectively collected serum samples 
      from 121 patients with non-infectious scleritis in a multicenter cohort study in 
      the Netherlands. In addition, healthy (n = 39) and uveitis controls (n = 48) were 
      included. Serum samples were tested for anti-native human type II collagen 
      antibodies using a validated enzyme-linked immunosorbent assay (ELISA). Further, 
      sclera-specific antibodies were determined using indirect immunofluorescence 
      (IIF) on primate retinal/scleral cryosections. Lastly, human leukocyte antigen 
      (HLA) typing was performed in 111 patients with scleritis. Anti-type II collagen 
      antibodies were found in 13% of scleritis patients, in 10% of healthy controls 
      and in 11% of uveitis controls (p = 0.91). A specific reaction to scleral nerve 
      tissue on IIF was observed in 33% of patients with scleritis, which was higher 
      than in healthy controls (11%; p = 0.01), but similar to uveitis controls (25%; 
      p = 0.36). Reactivity to the scleral nerve tissue was significantly associated 
      with earlier onset of scleritis (48 versus 56 years; p < 0.001), bilateral 
      involvement (65% versus 42%; p = 0.01), and less frequent development of scleral 
      necrosis (5% versus 22%; p = 0.02). HLA-B27 was found to be twice as prevalent in 
      patients with scleritis (15.3%) compared to a healthy population (7.2%). In 
      conclusion, scleral nerve autoantibody reactivity was more common in scleritis 
      and uveitis patients in contrast to healthy controls. Further research is needed 
      to characterize these scleral-nerve directed antibodies and assess their clinical 
      value.
CI  - Copyright (c) 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Vergouwen, Daphne P C
AU  - Vergouwen DPC
AD  - Department of Ophthalmology, Erasmus MC, University Medical Center Rotterdam, 
      Rotterdam, the Netherlands; Department of Immunology, Laboratory Medical 
      Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, the 
      Netherlands. Electronic address: d.vergouwen@erasmusmc.nl.
FAU - van Beek, Adriaan A
AU  - van Beek AA
AD  - Department of Immunology, HLA laboratory, Leiden University Medical Center, 
      Leiden, the Netherlands.
FAU - de Hoog, Joeri
AU  - de Hoog J
AD  - Department of Ophthalmology, Amsterdam UMC, Amsterdam, the Netherlands.
FAU - de Boer, Joke H
AU  - de Boer JH
AD  - Department of Ophthalmology, UMC Utrecht, Utrecht, the Netherlands.
FAU - Los, Leonoor I
AU  - Los LI
AD  - Department of Ophthalmology, UMC Groningen, Groningen, the Netherlands.
FAU - Gijs, Marlies
AU  - Gijs M
AD  - University Eye Clinic, Maastricht UMC+, Maastricht, the Netherlands.
FAU - Erckens, Roel J
AU  - Erckens RJ
AD  - University Eye Clinic, Maastricht UMC+, Maastricht, the Netherlands.
FAU - Verdijk, Rob M
AU  - Verdijk RM
AD  - Department of Pathology, Erasmus MC, University Medical Center Rotterdam, 
      Rotterdam, the Netherlands.
FAU - Haasnoot, Geert W
AU  - Haasnoot GW
AD  - Eurotransplant Reference Laboratory, Leiden University Medical Center, Leiden, 
      the Netherlands.
FAU - Roelen, Dave L
AU  - Roelen DL
AD  - Department of Immunology, HLA laboratory, Leiden University Medical Center, 
      Leiden, the Netherlands.
FAU - Rothova, Aniki
AU  - Rothova A
AD  - Department of Ophthalmology, Erasmus MC, University Medical Center Rotterdam, 
      Rotterdam, the Netherlands.
FAU - Ronnelid, Johan
AU  - Ronnelid J
AD  - Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, 
      Sweden.
FAU - Ten Berge, Josianne C
AU  - Ten Berge JC
AD  - Department of Ophthalmology, Erasmus MC, University Medical Center Rotterdam, 
      Rotterdam, the Netherlands.
FAU - Schreurs, Marco W J
AU  - Schreurs MWJ
AD  - Department of Immunology, Laboratory Medical Immunology, Erasmus MC, University 
      Medical Center Rotterdam, Rotterdam, the Netherlands.
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
DEP - 20240217
PL  - England
TA  - J Autoimmun
JT  - Journal of autoimmunity
JID - 8812164
SB  - IM
MH  - Animals
MH  - Humans
MH  - Autoimmunity
MH  - Cohort Studies
MH  - Sclera/pathology
MH  - *Scleritis/pathology
MH  - *Uveitis/pathology
OTO - NOTNLM
OT  - Autoantibodies
OT  - Autoimmunity
OT  - HLA-Association
OT  - Scleritis
OT  - Type II collagen antibodies
EDAT- 2024/02/19 00:42
MHDA- 2024/04/17 06:43
CRDT- 2024/02/18 18:08
PHST- 2023/07/05 00:00 [received]
PHST- 2023/12/29 00:00 [revised]
PHST- 2024/02/08 00:00 [accepted]
PHST- 2024/04/17 06:43 [medline]
PHST- 2024/02/19 00:42 [pubmed]
PHST- 2024/02/18 18:08 [entrez]
AID - S0896-8411(24)00012-X [pii]
AID - 10.1016/j.jaut.2024.103178 [doi]
PST - ppublish
SO  - J Autoimmun. 2024 Apr;144:103178. doi: 10.1016/j.jaut.2024.103178. Epub 2024 Feb 
      17.