PMID- 38372097
OWN - NLM
STAT- MEDLINE
DCOM- 20240220
LR  - 20240321
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 70
IP  - 1
DP  - 2024 Jan 31
TI  - Exosomal circMACF1 drives PI3K/AKT/mTOR-mediated autophagy suppression in 
      laryngeal squamous cell carcinoma.
PG  - 179-185
LID - 10.14715/cmb/2024.70.1.24 [doi]
AB  - Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor. The 
      regulatory functions of circular RNAs (circRNAs) in cancers have been broadly 
      reported. The hsa_circ_0011773 (circMACF1) is reported to be overexpressed in 
      LSCC tissues, while its biological function in LSCC remains unclear. CircMACF1 
      expression in LSCC tissues and cells was assessed via RT-qPCR. Exosomes extracted 
      from cells were identified by TEM and NTA. Autophagy-related proteins were tested 
      by western blot. Confocal microscope was employed for analyzing LC3 expression. 
      Cell proliferation, migration, and invasion were assessed by CCK-8 assay and 
      transwell assay. The levels of main proteins on PI3K/AKT/mTOR were tested by 
      western blot. We observed that circMACF1 was highly expressed in LSCC tissues and 
      cells. Furthermore, circMACF1 expression was also upregulated in the exosomes 
      derived from LSCC cells. CircMACF1 depletion promoted LC3 expression in cells. 
      Additionally, we proved that circMACF1 knockdown suppressed LSCC cell 
      proliferative, migratory and invasive capabilities via promoting autophagy. 
      Exosomal circMACF1 was found to promote LSCC tumor growth. Then, we proved that 
      circMACF1 could activate PI3K/AKT/mTOR pathway to regulate autophagy. Moreover, 
      MACF1 was positively regulated by circMACF1 and its overexpression notably 
      reversed the effects of circMACF1 depletion in LSCC progression. Exosomal 
      circMACF1 can regulate PI3K/AKT/mTOR-mediated autophagy suppression to facilitate 
      LSCC development.
FAU - Zhu, Yingnan
AU  - Zhu Y
AD  - Department of Otolaryngology, Maternal and Child Health Hospital of Hubei 
      Province, Tongji Medical College, Huazhong University of Science and Technology, 
      Wuhan, Hubei 430070, China. W13545345823@126.com.
FAU - Duan, Chuanxin
AU  - Duan C
AD  - Department of Otolaryngology, Maternal and Child Health Hospital of Hubei 
      Province, Tongji Medical College, Huazhong University of Science and Technology, 
      Wuhan, Hubei 430070, China. tyhwh@126.com.
FAU - Gui, Yunzhi
AU  - Gui Y
AD  - Department of Otolaryngology, Maternal and Child Health Hospital of Hubei 
      Province, Tongji Medical College, Huazhong University of Science and Technology, 
      Wuhan, Hubei 430070, China. gyz8112022@126.com.
FAU - Chen, Danxia
AU  - Chen D
AD  - Department of Otolaryngology, Maternal and Child Health Hospital of Hubei 
      Province, Tongji Medical College, Huazhong University of Science and Technology, 
      Wuhan, Hubei 430070, China. 2644704239@qq.com.
FAU - Su, Xi
AU  - Su X
AD  - Department of Otolaryngology, Maternal and Child Health Hospital of Hubei 
      Province, Tongji Medical College, Huazhong University of Science and Technology, 
      Wuhan, Hubei 430070, China. suxi20230803@163.com.
LA  - eng
PT  - Journal Article
DEP - 20240131
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - 0 (MACF1 protein, human)
RN  - 0 (RNA, Circular)
SB  - IM
MH  - Humans
MH  - Autophagy/genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - *Laryngeal Neoplasms/genetics/pathology
MH  - Phosphatidylinositol 3-Kinases/genetics/metabolism
MH  - Proto-Oncogene Proteins c-akt/genetics/metabolism
MH  - *Squamous Cell Carcinoma of Head and Neck/genetics/pathology
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
MH  - *RNA, Circular/genetics
EDAT- 2024/02/19 06:43
MHDA- 2024/02/20 11:57
CRDT- 2024/02/19 04:50
PHST- 2023/11/29 00:00 [received]
PHST- 2024/02/20 11:57 [medline]
PHST- 2024/02/19 06:43 [pubmed]
PHST- 2024/02/19 04:50 [entrez]
AID - 10.14715/cmb/2024.70.1.24 [doi]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):179-185. doi: 
      10.14715/cmb/2024.70.1.24.