PMID- 38372126
OWN - NLM
STAT- MEDLINE
DCOM- 20240319
LR  - 20240319
IS  - 1522-1490 (Electronic)
IS  - 0363-6119 (Linking)
VI  - 326
IP  - 4
DP  - 2024 Apr 1
TI  - Evolved changes in phenotype across skeletal muscles in deer mice native to high 
      altitude.
PG  - R297-R310
LID - 10.1152/ajpregu.00206.2023 [doi]
AB  - The cold and hypoxic conditions at high altitude necessitate high metabolic O(2) 
      demands to support thermogenesis while hypoxia reduces O(2) availability. 
      Skeletal muscles play key roles in thermogenesis, but our appreciation of muscle 
      plasticity and adaptation at high altitude has been hindered by past emphasis on 
      only a small number of muscles. We examined this issue in deer mice (Peromyscus 
      maniculatus). Mice derived from both high-altitude and low-altitude populations 
      were born and raised in captivity and then acclimated as adults to normoxia or 
      hypobaric hypoxia (12 kPa O(2) for 6-8 wk). Maximal activities of citrate 
      synthase (CS), cytochrome c oxidase (COX), beta-hydroxyacyl-CoA dehydrogenase 
      (HOAD), hexokinase (HK), pyruvate kinase (PK), and lactate dehydrogenase (LDH) 
      were measured in 20 muscles involved in shivering, locomotion, body posture, 
      ventilation, and mastication. Principal components analysis revealed an overall 
      difference in muscle phenotype between populations but no effect of hypoxia 
      acclimation. High-altitude mice had greater activities of mitochondrial enzymes 
      and/or lower activities of PK or LDH across many (but not all) respiratory, limb, 
      core and mastication muscles compared with low-altitude mice. In contrast, 
      chronic hypoxia had very few effects across muscles. Further examination of CS in 
      the gastrocnemius showed that population differences in enzyme activity stemmed 
      from differences in protein abundance and mRNA expression but not from population 
      differences in CS amino acid sequence. Overall, our results suggest that evolved 
      increases in oxidative capacity across many skeletal muscles, at least partially 
      driven by differences in transcriptional regulation, may contribute to 
      high-altitude adaptation in deer mice.NEW & NOTEWORTHY Most previous studies of 
      muscle plasticity and adaptation in high-altitude environments have focused on a 
      very limited number of skeletal muscles. Comparing high-altitude versus 
      low-altitude populations of deer mice, we show that a large number of muscles 
      involved in shivering, locomotion, body posture, ventilation, and mastication 
      exhibit greater mitochondrial enzyme activities in the high-altitude population. 
      Therefore, evolved increases in mitochondrial oxidative capacity across skeletal 
      muscles contribute to high-altitude adaptation.
FAU - Garrett, Emily J
AU  - Garrett EJ
AD  - Department of Biology, McMaster University, Hamilton, Ontario, Canada. ROR: 
      https://ror.org/02fa3aq29
FAU - Prasad, Srikripa K
AU  - Prasad SK
AD  - Department of Biology, McMaster University, Hamilton, Ontario, Canada. ROR: 
      https://ror.org/02fa3aq29
FAU - Schweizer, Rena M
AU  - Schweizer RM
AD  - Division of Biological Sciences, University of Montana, Missoula, Montana, United 
      States.
AD  - United States Department of Agriculture, Agricultural Research Service, 
      Pollinating Insects Research Unit, Utah State University, Logan, Utah, United 
      States.
FAU - McClelland, Grant B
AU  - McClelland GB
AUID- ORCID: 0000-0003-1500-9983
AD  - Department of Biology, McMaster University, Hamilton, Ontario, Canada. ROR: 
      https://ror.org/02fa3aq29
FAU - Scott, Graham R
AU  - Scott GR
AUID- ORCID: 0000-0002-4225-7475
AD  - Department of Biology, McMaster University, Hamilton, Ontario, Canada. ROR: 
      https://ror.org/02fa3aq29
LA  - eng
GR  - RGPIN-2018-05707/Canadian Government | Natural Sciences and Engineering Research 
      Council of Canada (NSERC)/
GR  - RGPIN-462246-2014/Canadian Government | Natural Sciences and Engineering Research 
      Council of Canada (NSERC)/
PT  - Journal Article
DEP - 20240219
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
SB  - IM
MH  - Animals
MH  - *Altitude
MH  - *Peromyscus/physiology
MH  - Hypoxia/metabolism
MH  - Muscle, Skeletal/metabolism
MH  - Acclimatization
MH  - Phenotype
OTO - NOTNLM
OT  - acclimatization
OT  - evolutionary physiology
OT  - high elevation
OT  - muscle metabolism
OT  - shivering thermogenesis
EDAT- 2024/02/19 06:42
MHDA- 2024/03/19 06:44
CRDT- 2024/02/19 05:29
PHST- 2024/03/19 06:44 [medline]
PHST- 2024/02/19 06:42 [pubmed]
PHST- 2024/02/19 05:29 [entrez]
AID - 10.1152/ajpregu.00206.2023 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2024 Apr 1;326(4):R297-R310. doi: 
      10.1152/ajpregu.00206.2023. Epub 2024 Feb 19.