PMID- 38373741
OWN - NLM
STAT- MEDLINE
DCOM- 20240223
LR  - 20240325
IS  - 1747-0285 (Electronic)
IS  - 1747-0277 (Linking)
VI  - 103
IP  - 2
DP  - 2024 Feb
TI  - Icariin promotes osteogenic differentiation of human bone marrow mesenchymal stem 
      cells by regulating USP47/SIRT1/Wnt/beta-catenin.
PG  - e14431
LID - 10.1111/cbdd.14431 [doi]
AB  - Icariin has been shown to promote osteogenic differentiation of bone marrow 
      mesenchymal stem cells (BMSCs). However, the underlying molecular mechanism by 
      which Icariin regulates osteogenic differentiation needs to be further revealed. 
      The viability of BMSCs was assessed by cell counting kit 8 assay. BMSC osteogenic 
      differentiation ability was evaluated by detecting alkaline phosphatase activity 
      and performing alizarin red S staining. The protein levels of osteogenic 
      differentiation-related markers, sirtuin 1 (SIRT1), ubiquitin-specific protease 
      47 (USP47), and Wnt/beta-catenin-related markers were determined using western blot. 
      SIRT1 mRNA level was measured using quantitative real-time PCR. The regulation of 
      USP47 on SIRT1 was confirmed by ubiquitination detection and 
      co-immunoprecipitation analysis. Icariin could promote BMSC osteogenic 
      differentiation. SIRT1 expression was enhanced by Icariin, and its knockdown 
      suppressed Icariin-induced BMSC osteogenic differentiation. Moreover, 
      deubiquitinating enzyme USP47 could stabilize SIRT1 protein expression. Besides, 
      SIRT1 overexpression reversed the inhibiting effect of USP47 knockdown on BMSC 
      osteogenic differentiation, and USP47 knockdown also restrained Icariin-induced 
      BMSC osteogenic differentiation. Additionally, Icariin enhanced the activity of 
      the Wnt/beta-catenin pathway by upregulating SIRT1. Icariin facilitated BMSC 
      osteogenic differentiation via the USP47/SIRT1/Wnt/beta-catenin pathway.
CI  - (c) 2024 John Wiley & Sons A/S.
FAU - Wang, Hongrui
AU  - Wang H
AD  - Department of Orthopedics, First Affiliated Hospital of Naval Medical University, 
      Shanghai, China.
AD  - Department of Orthopedics, The Second Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu, China.
FAU - Zhang, Hongyue
AU  - Zhang H
AD  - Department of Orthopedics, First Affiliated Hospital of Naval Medical University, 
      Shanghai, China.
FAU - Zhang, Yuntong
AU  - Zhang Y
AD  - Department of Orthopedics, First Affiliated Hospital of Naval Medical University, 
      Shanghai, China.
FAU - Wang, Panfeng
AU  - Wang P
AD  - Department of Orthopedics, First Affiliated Hospital of Naval Medical University, 
      Shanghai, China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Chem Biol Drug Des
JT  - Chemical biology & drug design
JID - 101262549
RN  - 0 (beta Catenin)
RN  - 0 (Flavonoids)
RN  - VNM47R2QSQ (icariin)
RN  - EC 3.5.1.- (SIRT1 protein, human)
RN  - EC 3.5.1.- (Sirtuin 1)
RN  - 0 (USP47 protein, human)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
RN  - EC 3.4.19.12 (Ubiquitin-Specific Proteases)
SB  - IM
MH  - Humans
MH  - beta Catenin/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - *Flavonoids/pharmacology
MH  - *Mesenchymal Stem Cells/cytology/drug effects/metabolism
MH  - *Osteogenesis/drug effects/genetics
MH  - *Sirtuin 1/genetics/metabolism
MH  - Ubiquitin Thiolesterase/metabolism
MH  - Ubiquitin-Specific Proteases/metabolism
MH  - Gene Knockdown Techniques
OTO - NOTNLM
OT  - BMSCs
OT  - Icariin
OT  - SIRT1
OT  - USP47
OT  - osteogenic differentiation
EDAT- 2024/02/20 11:50
MHDA- 2024/02/21 11:22
CRDT- 2024/02/19 20:22
PHST- 2023/11/30 00:00 [revised]
PHST- 2023/09/12 00:00 [received]
PHST- 2023/12/18 00:00 [accepted]
PHST- 2024/02/21 11:22 [medline]
PHST- 2024/02/20 11:50 [pubmed]
PHST- 2024/02/19 20:22 [entrez]
AID - 10.1111/cbdd.14431 [doi]
PST - ppublish
SO  - Chem Biol Drug Des. 2024 Feb;103(2):e14431. doi: 10.1111/cbdd.14431.