PMID- 38378057
OWN - NLM
STAT- MEDLINE
DCOM- 20240311
LR  - 20240311
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 921
DP  - 2024 Apr 15
TI  - Biogenic calcium improved Cd(2+) and Pb(2+) immobilization in soil using the 
      ureolytic bacteria Bacillus pasteurii.
PG  - 171060
LID - S0048-9697(24)01199-9 [pii]
LID - 10.1016/j.scitotenv.2024.171060 [doi]
AB  - Bioremediation based on microbial-induced carbonate precipitation (MICP) was 
      conducted in cadmium and lead contaminated soil to investigate the effects of 
      MICP on Cd and Pb in soil. In this study, soil indigenous nitrogen was shown to 
      induce MICP to stabilize heavy metals without inputting exogenous urea. The 
      results showed that applying Bacillus pasteurii coupled with CaCl(2) reduced Cd 
      and Pb bioavailability, which could be clarified through the proportion of 
      exchangeable Cd and Pb in soil decreasing by 23.65 % and 12.76 %, respectively. 
      Moreover, B. pasteurii was combined separately with hydroxyapatite (HAP), 
      eggshells (ES), and oyster shells (OS) to investigate their effects on soil heavy 
      metals' chemical fractions, toxicity characteristic leaching procedure 
      (TCLP)-extractable Cd and Pb as well as enzymatic activity. Results showed that 
      applying B. pasteurii in soil significantly decreased the heavy metals in the 
      exchangeable fraction and increased them in the carbonate phase fraction. When B. 
      pasteurii was combined with ES and OS, the content of carbonate-bound Cd 
      increased by 114.72 % and 118.81 %, respectively, significantly higher than when 
      B. pasteurii was combined with HAP, wherein the fraction of carbonate-bound Cd 
      increased by 86 %. The combination of B. pasteurii and biogenic calcium 
      effectively reduced the leached contents of Cd and Pb in soil, and the 
      TCLP-extractable Cd and Pb fractions decreased by 43.88 % and 30.66 %, 
      respectively, in the BP + ES group and by 52.60 % and 41.77 %, respectively, in 
      the BP + OS group. This proved that MICP reduced heavy metal bioavailability in 
      the soil. Meanwhile, applying B. pasteurii and calcium materials significantly 
      increased the soil urease enzyme activity. The microstructure and chemical 
      composition of the soil samples were studied, and the results from scanning 
      electron microscope, Fourier transform infra-red spectroscopy, and X-ray 
      diffraction demonstrated the MICP process and identified the formation of 
      CaCO(3), Ca(0.67)Cd(0.33)CO(3), and PbCO(3) in heavy metal-contaminated soil.
CI  - Copyright (c) 2024 Elsevier B.V. All rights reserved.
FAU - Zhang, Li
AU  - Zhang L
AD  - Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution 
      Prevention, College of Resources and Environment, Anhui Agricultural University, 
      Hefei 230036, China.
FAU - Wang, Wenjun
AU  - Wang W
AD  - Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution 
      Prevention, College of Resources and Environment, Anhui Agricultural University, 
      Hefei 230036, China.
FAU - Yue, Caili
AU  - Yue C
AD  - Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution 
      Prevention, College of Resources and Environment, Anhui Agricultural University, 
      Hefei 230036, China.
FAU - Si, Youbin
AU  - Si Y
AD  - Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution 
      Prevention, College of Resources and Environment, Anhui Agricultural University, 
      Hefei 230036, China. Electronic address: youbinsi@ahau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20240218
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 00BH33GNGH (Cadmium)
RN  - SY7Q814VUP (Calcium)
RN  - 2P299V784P (Lead)
RN  - 0 (Soil)
RN  - 0 (Metals, Heavy)
RN  - H0G9379FGK (Calcium Carbonate)
RN  - 0 (Carbonates)
RN  - 0 (Soil Pollutants)
RN  - Sporosarcina pasteurii
SB  - IM
MH  - Cadmium/analysis
MH  - Calcium
MH  - Lead
MH  - Soil/chemistry
MH  - *Metals, Heavy/analysis
MH  - Bacteria
MH  - Calcium Carbonate
MH  - Biodegradation, Environmental
MH  - Carbonates
MH  - *Soil Pollutants/analysis
MH  - *Sporosarcina
OTO - NOTNLM
OT  - Bacillus pasteurii
OT  - Biogenic calcium
OT  - Biomineralization
OT  - Heavy metals
OT  - Soil
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2024/02/21 11:14
MHDA- 2024/03/11 06:43
CRDT- 2024/02/20 19:10
PHST- 2023/09/14 00:00 [received]
PHST- 2023/11/15 00:00 [revised]
PHST- 2024/02/15 00:00 [accepted]
PHST- 2024/03/11 06:43 [medline]
PHST- 2024/02/21 11:14 [pubmed]
PHST- 2024/02/20 19:10 [entrez]
AID - S0048-9697(24)01199-9 [pii]
AID - 10.1016/j.scitotenv.2024.171060 [doi]
PST - ppublish
SO  - Sci Total Environ. 2024 Apr 15;921:171060. doi: 10.1016/j.scitotenv.2024.171060. 
      Epub 2024 Feb 18.