PMID- 38378109
OWN - NLM
STAT- MEDLINE
DCOM- 20240327
LR  - 20240327
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 263
IP  - Pt 1
DP  - 2024 Apr
TI  - Comparison of extraction processes, characterization and intestinal protection 
      activity of Bletilla striata polysaccharides.
PG  - 130267
LID - S0141-8130(24)01070-5 [pii]
LID - 10.1016/j.ijbiomac.2024.130267 [doi]
AB  - We optimized the extraction process of Bletilla striata polysaccharides using 
      orthogonal design, Box-Behnken design (BBD), and genetic algorithm-back 
      propagation (GA-BP), then compared and evaluated them to confirm that the 
      combination of BBD and GA-BP neural networks was capable of increasing 
      polysaccharide yields and antioxidant activity. The optimal extraction parameters 
      were as follows: liquid-to-solid ratio of 15 mL/g, extraction power of 450 W, and 
      extraction time of 34 min. Under these conditions, the polysaccharide yield and 
      antioxidant activity were 8.29 +/- 0.50 % and 26.20 +/- 0.28 (mM FE/mg). 
      Subsequently, the polysaccharide was purified to obtain purified Bletilla striata 
      polysaccharides 1 (pBSP1) with a Mw of 255.172 kDa. Scanning electron microscope 
      (SEM), ultraviolet-visible detector (UV), fourier transform infrared spectrometer 
      (FTIR), high performance liquid chromatography (HPLC), X-ray diffraction (XRD), 
      nuclear magnetic resonance (NMR) and periodate oxidation were used to analyze the 
      structure of pBSP1. The results showed pBSP1 had a smooth surface and a rough 
      interior, with a composition of alpha-D conformation glucose (18.23 %) and beta-D 
      conformation mannose (53.77 %), and an amorphous crystal structure. According to 
      the results of thermogravimetric and rheological tests, pBSP1 exhibits good 
      thermal stability and viscoelastic behavior. Furthermore, pBSP1 protected 
      lipopolysaccharide (LPS)-induced GES - 1 and Caco2 cells, the results showed 
      pBSP1(400 mug/mL) lowered TEER synthesis in Caco2 cells as well as apoptosis and 
      reactive oxygen species (ROS) production in both cells, indicating that pBSP1 may 
      have an intestine protective effect.
CI  - Copyright (c) 2024. Published by Elsevier B.V.
FAU - Qiu, Junjie
AU  - Qiu J
AD  - School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 
      310053, China.
FAU - Xu, Xiao
AU  - Xu X
AD  - Asset Management Co., Ltd, Zhejiang Chinese Medical University, Hangzhou 310053, 
      China.
FAU - Guo, Jingyan
AU  - Guo J
AD  - School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 
      310053, China.
FAU - Wang, Zhenyu
AU  - Wang Z
AD  - School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 
      310053, China.
FAU - Wu, Jinjin
AU  - Wu J
AD  - The Third School of Clinical Medicine, Zhejiang Chinese Medical University, 
      Hangzhou, China.
FAU - Ding, Huiqin
AU  - Ding H
AD  - The Second School of Clinical Medicine, Zhejiang Chinese Medical University, 
      Hangzhou, China.
FAU - Xu, Yuchen
AU  - Xu Y
AD  - School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 
      310053, China.
FAU - Wu, Yili
AU  - Wu Y
AD  - School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 
      310053, China.
FAU - Ying, Qianyi
AU  - Ying Q
AD  - School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 
      310053, China.
FAU - Qiu, Jiawei
AU  - Qiu J
AD  - School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 
      310053, China.
FAU - Wu, Suxiang
AU  - Wu S
AD  - School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 
      310053, China. Electronic address: wsx173@126.com.
FAU - Shi, Senlin
AU  - Shi S
AD  - School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 
      310053, China. Electronic address: pjstone@zcmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20240218
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Antioxidants)
RN  - IY9XDZ35W2 (Glucose)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Humans
MH  - *Antioxidants/pharmacology/chemistry
MH  - Caco-2 Cells
MH  - Oxidation-Reduction
MH  - Glucose
MH  - Polysaccharides/pharmacology/chemistry
MH  - *Orchidaceae/chemistry
OTO - NOTNLM
OT  - Characterization
OT  - Extraction
OT  - Intestinal protection
COIS- Declaration of competing interest The authors declare no conflicts of interest.
EDAT- 2024/02/21 11:14
MHDA- 2024/03/27 06:44
CRDT- 2024/02/20 19:12
PHST- 2023/08/14 00:00 [received]
PHST- 2024/02/12 00:00 [revised]
PHST- 2024/02/15 00:00 [accepted]
PHST- 2024/03/27 06:44 [medline]
PHST- 2024/02/21 11:14 [pubmed]
PHST- 2024/02/20 19:12 [entrez]
AID - S0141-8130(24)01070-5 [pii]
AID - 10.1016/j.ijbiomac.2024.130267 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2024 Apr;263(Pt 1):130267. doi: 
      10.1016/j.ijbiomac.2024.130267. Epub 2024 Feb 18.