PMID- 38378443
OWN - NLM
STAT- MEDLINE
DCOM- 20240222
LR  - 20240223
IS  - 2150-5608 (Electronic)
IS  - 2150-5594 (Print)
IS  - 2150-5594 (Linking)
VI  - 15
IP  - 1
DP  - 2024 Dec
TI  - Microbiome analysis reveals the inducing effect of Pseudomonas on prostatic 
      hyperplasia via activating NF-kappaB signalling.
PG  - 2313410
LID - 10.1080/21505594.2024.2313410 [doi]
LID - 2313410
AB  - Benign prostatic hyperplasia (BPH) is a prevalent disease among middle-aged and 
      elderly males, but its pathogenesis remains unclear. Dysbiosis of the microbiome 
      is increasingly recognized as a significant factor in various human diseases. 
      Prostate tissue also contains a unique microbiome, and its dysbiosis has been 
      proposed to contribute to prostate diseases. Here, we obtained prostate tissues 
      and preoperative catheterized urine from 24 BPH individuals, and 8 normal 
      prostate samples as controls, which followed strict aseptic measures. Using 
      metagenomic next-generation sequencing (mNGS), we found the disparities in the 
      microbiome composition between normal and BPH tissues, with Pseudomonas 
      significantly enriched in BPH tissues, as confirmed by fluorescence in situ 
      hybridization (FISH). Additionally, we showed that the prostate microbiome 
      differed from the urine microbiome. In vitro experiments revealed that 
      lipopolysaccharide (LPS) of Pseudomonas activated NF-kappaB signalling, leading to 
      inflammation, proliferation, and EMT processes, while inhibiting apoptosis in 
      prostatic cells. Overall, our research determines the presence of microbiome 
      dysbiosis in BPH, and suggests that Pseudomonas, as the dominant microflora, may 
      promote the progression of BPH through LPS activation of NF-kappaB signalling.
FAU - Li, Jiaren
AU  - Li J
AD  - Department of Urology, The Third Xiangya Hospital, Central South University, 
      Changsha, Hunan, China.
FAU - Li, Youyou
AU  - Li Y
AD  - Department of Urology, The Third Xiangya Hospital, Central South University, 
      Changsha, Hunan, China.
FAU - Zhou, Liang
AU  - Zhou L
AD  - Department of Urology, The Third Xiangya Hospital, Central South University, 
      Changsha, Hunan, China.
FAU - Li, Hongming
AU  - Li H
AD  - Movement System Injury and Repair Research Center, Xiangya Hospital, Central 
      South University, Changsha, Hunan, China.
FAU - Wan, Tengfei
AU  - Wan T
AD  - Movement System Injury and Repair Research Center, Xiangya Hospital, Central 
      South University, Changsha, Hunan, China.
FAU - Tang, Jin
AU  - Tang J
AD  - Department of Urology, The Third Xiangya Hospital, Central South University, 
      Changsha, Hunan, China.
FAU - Zhou, Lei
AU  - Zhou L
AD  - Department of Urology, The Third Xiangya Hospital, Central South University, 
      Changsha, Hunan, China.
FAU - Xie, Hui
AU  - Xie H
AD  - Movement System Injury and Repair Research Center, Xiangya Hospital, Central 
      South University, Changsha, Hunan, China.
FAU - Wang, Long
AU  - Wang L
AUID- ORCID: 0000-0003-4583-2428
AD  - Department of Urology, The Third Xiangya Hospital, Central South University, 
      Changsha, Hunan, China.
LA  - eng
PT  - Journal Article
DEP - 20240220
PL  - United States
TA  - Virulence
JT  - Virulence
JID - 101531386
RN  - 0 (NF-kappa B)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Male
MH  - Middle Aged
MH  - Aged
MH  - Humans
MH  - *Prostatic Hyperplasia/pathology
MH  - NF-kappa B/genetics
MH  - Pseudomonas
MH  - Dysbiosis
MH  - In Situ Hybridization, Fluorescence
MH  - Lipopolysaccharides
MH  - *Microbiota
PMC - PMC10880505
OTO - NOTNLM
OT  - Benign prostatic hyperplasia
OT  - NF-kappaB
OT  - Pseudomonas
OT  - lipopolysaccharide
OT  - microbiome
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2024/02/21 11:14
MHDA- 2024/02/22 06:43
PMCR- 2024/02/20
CRDT- 2024/02/20 23:31
PHST- 2024/02/22 06:43 [medline]
PHST- 2024/02/21 11:14 [pubmed]
PHST- 2024/02/20 23:31 [entrez]
PHST- 2024/02/20 00:00 [pmc-release]
AID - 2313410 [pii]
AID - 10.1080/21505594.2024.2313410 [doi]
PST - ppublish
SO  - Virulence. 2024 Dec;15(1):2313410. doi: 10.1080/21505594.2024.2313410. Epub 2024 
      Feb 20.