PMID- 38379463
OWN - NLM
STAT- MEDLINE
DCOM- 20240423
LR  - 20240423
IS  - 1751-553X (Electronic)
IS  - 1751-5521 (Linking)
VI  - 46
IP  - 3
DP  - 2024 Jun
TI  - Imaging flow cytometric detection of del(17p) in bone marrow and circulating 
      plasma cells in multiple myeloma.
PG  - 495-502
LID - 10.1111/ijlh.14248 [doi]
AB  - BACKGROUND: Detection of del(17p) in myeloma is generally performed by 
      fluorescence in situ hybridization (FISH) on a slide with analysis of up to 200 
      nuclei. The small cell sample analyzed makes this a low precision test. We report 
      the utility of an automated FISH method, called "immuno-flowFISH", to detect 
      plasma cells with adverse prognostic risk del(17p) in bone marrow and blood 
      samples of patients with myeloma. METHODS: Bone marrow (n = 31) and blood 
      (n = 19) samples from 35 patients with myeloma were analyzed using 
      immuno-flowFISH. Plasma cells were identified by CD38/CD138-immunophenotypic 
      gating and assessed for the 17p locus and centromere of chromosome 17. Cells were 
      acquired on an AMNIS ImageStreamX MkII imaging flow cytometer using INSPIRE 
      software. RESULTS: Chromosome 17 abnormalities were identified in 
      CD38/CD138-positive cells in bone marrow (6/31) and blood (4/19) samples when the 
      percent plasma cell burden ranged from 0.03% to 100% of cells. Abnormalities 
      could be identified in 14.5%-100% of plasma cells. CONCLUSIONS: The 
      "immuno-flowFISH" imaging flow cytometric method could detect del(17p) in plasma 
      cells in both bone marrow and blood samples of myeloma patients. This method was 
      also able to detect gains and losses of chromosome 17, which are also of 
      prognostic significance. The lowest levels of 0.009% (bone marrow) and 0.001% 
      (blood) for chromosome 17 abnormalities was below the detection limit of current 
      FISH method. This method offers potential as a new means of identifying these 
      prognostically important chromosomal defects, even when only rare cells are 
      present and for serial disease monitoring.
CI  - (c) 2024 The Authors. International Journal of Laboratory Hematology published by 
      John Wiley & Sons Ltd.
FAU - Mincherton, Thomas I
AU  - Mincherton TI
AUID- ORCID: 0000-0002-8347-4302
AD  - School of Biomedical Sciences, The University of Western Australia, Crawley, 
      Western Australia, Australia.
FAU - Lam, Stephanie J
AU  - Lam SJ
AD  - PathWest Laboratory Medicine, Nedlands, Western Australia, Australia.
AD  - Haematology Department, Fiona Stanley Hospital, Murdoch, Western Australia, 
      Australia.
FAU - Clarke, Sarah E
AU  - Clarke SE
AD  - School of Biomedical Sciences, The University of Western Australia, Crawley, 
      Western Australia, Australia.
AD  - PathWest Laboratory Medicine, Nedlands, Western Australia, Australia.
AD  - Haematology Department, Fiona Stanley Hospital, Murdoch, Western Australia, 
      Australia.
FAU - Hui, Henry Y L
AU  - Hui HYL
AUID- ORCID: 0000-0002-0442-6987
AD  - School of Biomedical Sciences, The University of Western Australia, Crawley, 
      Western Australia, Australia.
FAU - Malherbe, Jacques A J
AU  - Malherbe JAJ
AD  - School of Biomedical Sciences, The University of Western Australia, Crawley, 
      Western Australia, Australia.
AD  - Haematology Department, Fiona Stanley Hospital, Murdoch, Western Australia, 
      Australia.
FAU - Chuah, Hun S
AU  - Chuah HS
AD  - PathWest Laboratory Medicine, Nedlands, Western Australia, Australia.
AD  - Haematology Department, Royal Perth Hospital, Perth, Western Australia, 
      Australia.
FAU - Sidiqi, M Hasib
AU  - Sidiqi MH
AUID- ORCID: 0000-0003-1089-3853
AD  - PathWest Laboratory Medicine, Nedlands, Western Australia, Australia.
AD  - Haematology Department, Fiona Stanley Hospital, Murdoch, Western Australia, 
      Australia.
FAU - Fuller, Kathy A
AU  - Fuller KA
AUID- ORCID: 0000-0002-3462-7501
AD  - School of Biomedical Sciences, The University of Western Australia, Crawley, 
      Western Australia, Australia.
FAU - Erber, Wendy N
AU  - Erber WN
AUID- ORCID: 0000-0002-1028-9376
AD  - School of Biomedical Sciences, The University of Western Australia, Crawley, 
      Western Australia, Australia.
AD  - PathWest Laboratory Medicine, Nedlands, Western Australia, Australia.
LA  - eng
GR  - Australian Government Department of Education; Research Training Program (RTP) 
      Scholarship/
GR  - Government of Western Australian; Future Health Research and Innovation Fund/
GR  - Royal Perth Hospital Medical Research Foundation/
GR  - National Health and Medical Research Council/
PT  - Journal Article
DEP - 20240221
PL  - England
TA  - Int J Lab Hematol
JT  - International journal of laboratory hematology
JID - 101300213
SB  - IM
MH  - Humans
MH  - *Multiple Myeloma/diagnosis/genetics/blood/pathology
MH  - *Plasma Cells/pathology
MH  - *Flow Cytometry/methods
MH  - *In Situ Hybridization, Fluorescence
MH  - *Chromosomes, Human, Pair 17/genetics
MH  - Male
MH  - Female
MH  - Aged
MH  - Middle Aged
MH  - Bone Marrow/pathology
MH  - Chromosome Deletion
MH  - Aged, 80 and over
MH  - Immunophenotyping
MH  - Adult
OTO - NOTNLM
OT  - circulating plasma cells
OT  - del(17p)
OT  - fluorescence in situ hybridization
OT  - imaging flow cytometry
OT  - multiple myeloma
EDAT- 2024/02/21 11:15
MHDA- 2024/04/23 15:50
CRDT- 2024/02/21 03:33
PHST- 2023/11/27 00:00 [received]
PHST- 2024/02/04 00:00 [accepted]
PHST- 2024/04/23 15:50 [medline]
PHST- 2024/02/21 11:15 [pubmed]
PHST- 2024/02/21 03:33 [entrez]
AID - 10.1111/ijlh.14248 [doi]
PST - ppublish
SO  - Int J Lab Hematol. 2024 Jun;46(3):495-502. doi: 10.1111/ijlh.14248. Epub 2024 Feb 
      21.