PMID- 38381966
OWN - NLM
STAT- Publisher
LR  - 20240221
IS  - 0191-2917 (Print)
IS  - 0191-2917 (Linking)
DP  - 2024 Feb 21
TI  - Cytogenetic and genomic characterization of a novel wheat-tetraploid Thinopyrum 
      elongatum 1BS.1EL translocation line with stripe rust resistance.
LID - 10.1094/PDIS-12-23-2799-RE [doi]
AB  - Stripe rust, caused by Puccinia striiformis f. sp tritici (Pst), is a destructive 
      wheat disease pathogen. Thinopyrum elongatum is a valuable germplasm including 
      diploid, tetraploid, and decaploid with plenty of biotic and abiotic resistance. 
      In a previous study, we generated a stripe rust resistance wheat-tetraploid Th. 
      elongatum 1E/1D substitution line K17-841-1. To further apply the wild germplasm 
      for wheat breeding, we selected and obtained a new homozygous wheat-tetraploid 
      Th. elongatum translocation line T1BS.1EL using genomic in situ hybridization 
      (GISH), fluorescence in situ hybridization (FISH), oligo-FISH-Painting, and the 
      wheat 55K single nucleotide polymorphisms (SNPs) genotyping array. The T1BS.1EL 
      is highly resistant to stripe rust at the seedling and adult stage. Pedigree and 
      molecular marker analyses revealed that the resistance gene was located on 
      chromosome arm 1EL of tetraploid Th. elongatum, tentatively named Yr1EL. Besides, 
      we developed and validated 32 Simple Sequence Repeats (SSR) markers and two 
      kompititive allele specific PCR (KASP) assays which were specific to tetraploid 
      Th. elongatum chromosome arm 1EL to facilitate marker-assisted selection for 
      alien 1EL stripe rust resistance breeding. This will help us explore and locate 
      the stripe rust resistance gene mapping on the 1E chromosome and deploy it in the 
      wheat breeding program.
FAU - Wu, Dandan
AU  - Wu D
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, State Key Laboratory of 
      Crop Gene Exploration and Utilization in Southwest China, Chengdu, China.
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, Triticeae Research 
      Institute, Chengdu, Sichuan, China; 14646@sicau.edu.cn.
FAU - Zhao, Xin
AU  - Zhao X
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, State Key Laboratory of 
      Crop Gene Exploration and Utilization in Southwest China, Chengdu, Sichuan, 
      China.
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, Triticeae Research 
      Institute, Chengdu, Sichuan, China; 2765651980@qq.com.
FAU - Xie, Yangqiu
AU  - Xie Y
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, State Key Laboratory of 
      Crop Gene Exploration and Utilization in Southwest China, Chengdu, Sichuan, 
      China.
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, Triticeae Research 
      Institute, Chengdu, Sichuan, China; 18224463447@163.com.
FAU - Li, Lingyu
AU  - Li L
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, State Key Laboratory of 
      Crop Gene Exploration and Utilization in Southwest China, Chengdu, Sichuan, 
      China.
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, Triticeae Research 
      Institute, Chengdu, Sichuan, China; 847491286@qq.com.
FAU - Li, Yinghui
AU  - Li Y
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, State Key Laboratory of 
      Crop Gene Exploration and Utilization in Southwest China, Chengdu, Sichuan, 
      China.
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, Triticeae Research 
      Institute, Chengdu, Sichuan, China; liyinghui@sicau.edu.cn.
FAU - Zhu, Wei
AU  - Zhu W
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, State Key Laboratory of 
      Crop Gene Exploration and Utilization in Southwest China, Chengdu, Sichuan, 
      China.
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, Triticeae Research 
      Institute, Chengdu, Sichuan, China; zhuwei202209@163.com.
FAU - Xu, Lili
AU  - Xu L
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, Triticeae Research 
      Institute, Chengdu, Sichuan, China; xulili_0627@126.com.
FAU - Wang, Yi
AU  - Wang Y
AD  - Chengdu, State Key Laboratory of Crop Gene Exploration and Utilization in 
      Southwest China, China.
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, Triticeae Research 
      Institute, Chengdu, Sichuan, China; wangyi@sicau.edu.cn.
FAU - Zeng, Jian
AU  - Zeng J
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, College of Resources, 
      Chengdu, Sichuan, China; zengjian@sicau.edu.cn.
FAU - Cheng, Yiran
AU  - Cheng Y
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, State Key Laboratory of 
      Crop Gene Exploration and Utilization in Southwest China, Chengdu, Sichuan, 
      China; chengyiran@sicau.edu.cn.
FAU - Sha, Lina
AU  - Sha L
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, College of Grassland 
      Science and Technology, Chengdu, Sichuan, China; rice_shazhi@163.com.
FAU - Fan, Xing
AU  - Fan X
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, State Key Laboratory of 
      Crop Gene Exploration and Utilization in Southwest China, Chengdu, Sichuan, 
      China.
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, Triticeae Research 
      Institute, Chengdu, Sichuan, China; fanxing9988@163.com.
FAU - Zhang, Haiqin
AU  - Zhang H
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, College of Resources, 
      Chengdu, Sichuan, China; haiqinzhang@163.com.
FAU - Zhou, Yonghong
AU  - Zhou Y
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, State Key Laboratory of 
      Crop Gene Exploration and Utilization in Southwest China, Chengdu, Sichuan, 
      China.
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, Triticeae Research 
      Institute, Chengdu, Sichuan, China; zhouyh@sicau.edu.cn.
FAU - Kang, Huoyang
AU  - Kang H
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, State Key Laboratory of 
      Crop Gene Exploration and Utilization in Southwest China, Chengdu, Sichuan, 
      China.
AD  - Sichuan Agricultural University - Chengdu Campus, 506176, Triticeae Research 
      Institute, Chengdu, Sichuan, China; houyang.kang@sicau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20240221
PL  - United States
TA  - Plant Dis
JT  - Plant disease
JID - 9882809
SB  - IM
OTO - NOTNLM
OT  - Crop Type
OT  - Disease management
OT  - Epidemiology
OT  - Field crops
OT  - Subject Areas
OT  - cereals and grains
OT  - cultivar/resistance
OT  - disease development and spread
EDAT- 2024/02/21 18:43
MHDA- 2024/02/21 18:43
CRDT- 2024/02/21 15:23
PHST- 2024/02/21 18:43 [medline]
PHST- 2024/02/21 18:43 [pubmed]
PHST- 2024/02/21 15:23 [entrez]
AID - 10.1094/PDIS-12-23-2799-RE [doi]
PST - aheadofprint
SO  - Plant Dis. 2024 Feb 21. doi: 10.1094/PDIS-12-23-2799-RE.