PMID- 38386424
OWN - NLM
STAT- MEDLINE
DCOM- 20240307
LR  - 20240307
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 16
IP  - 9
DP  - 2024 Mar 6
TI  - Targeted Single Particle Tracking with Upconverting Nanoparticles.
PG  - 11217-11227
LID - 10.1021/acsami.3c17116 [doi]
AB  - Single particle tracking (SPT) is a powerful technique for real-time microscopic 
      visualization of the movement of individual biomolecules within or on the surface 
      of living cells. However, SPT often suffers from the suboptimal performance of 
      the photon-emitting labels used to tag the biomolecules of interest. For example, 
      fluorescent dyes have poor photostability, while quantum dots suffer from 
      blinking that hampers track acquisition and interpretation. Upconverting 
      nanoparticles (UCNPs) have recently emerged as a promising anti-Stokes 
      luminescent label for SPT. In this work, we demonstrated targeted SPT using 
      UCNPs. For this, we synthesized 30 nm diameter doped UCNPs and coated them with 
      amphiphilic polymers decorated with polyethylene glycol chains to make them 
      water-dispersible and minimize their nonspecific interactions with cells. Coated 
      UCNPs highly homogeneous in brightness (as confirmed by a single particle 
      investigation) were functionalized by immunoglobulin E (IgE) using a 
      biotin-streptavidin strategy. Using these IgE-UCNP SPT labels, we tracked 
      high-affinity IgE receptors (FcepsilonRI) on the membrane of living RBL-2H3 mast cells 
      at 37  degrees C in the presence and absence of antigen and obtained good agreement with 
      the literature. Moreover, we used the FcepsilonRI-IgE receptor-antibody system to 
      directly compare the performance of UCNP-based SPT labels to organic dyes 
      (AlexaFluor647) and quantum dots (QD655). Due to their photostability as well as 
      their backgroundless and continuous luminescence, SPT trajectories obtained with 
      UCNP labels are no longer limited by the photophysics of the label but only by 
      the dynamics of the system and, in particular, the movement of the label out of 
      the field of view and/or focal plane.
FAU - Dukhno, Oleksii
AU  - Dukhno O
AUID- ORCID: 0000-0001-6860-7042
AD  - Laboratory of Biomaging and Pathologies, UMR 7021 CNRS, University of Strasbourg, 
      Strasbourg 67000, France.
FAU - Ghosh, Srijayee
AU  - Ghosh S
AUID- ORCID: 0000-0003-2869-1993
AD  - Laboratory of Biomaging and Pathologies, UMR 7021 CNRS, University of Strasbourg, 
      Strasbourg 67000, France.
FAU - Greiner, Vanille
AU  - Greiner V
AD  - Laboratory of Biomaging and Pathologies, UMR 7021 CNRS, University of Strasbourg, 
      Strasbourg 67000, France.
FAU - Bou, Sophie
AU  - Bou S
AD  - Laboratory of Biomaging and Pathologies, UMR 7021 CNRS, University of Strasbourg, 
      Strasbourg 67000, France.
FAU - Godet, Julien
AU  - Godet J
AD  - Laboratory of Biomaging and Pathologies, UMR 7021 CNRS, University of Strasbourg, 
      Strasbourg 67000, France.
AD  - IMAGeS team at ICube, UMR 7357, CNRS, Universite de Strasbourg, Strasbourg 67000, 
      France.
FAU - Muhr, Verena
AU  - Muhr V
AD  - Institute of Analytical Chemistry, Chemo- and Biosensors, University of 
      Regensburg, 93040 Regensburg, Germany.
FAU - Buchner, Markus
AU  - Buchner M
AD  - Institute of Analytical Chemistry, Chemo- and Biosensors, University of 
      Regensburg, 93040 Regensburg, Germany.
FAU - Hirsch, Thomas
AU  - Hirsch T
AD  - Institute of Analytical Chemistry, Chemo- and Biosensors, University of 
      Regensburg, 93040 Regensburg, Germany.
FAU - Mely, Yves
AU  - Mely Y
AUID- ORCID: 0000-0001-7328-8269
AD  - Laboratory of Biomaging and Pathologies, UMR 7021 CNRS, University of Strasbourg, 
      Strasbourg 67000, France.
FAU - Przybilla, Frederic
AU  - Przybilla F
AUID- ORCID: 0009-0009-2016-353X
AD  - Laboratory of Biomaging and Pathologies, UMR 7021 CNRS, University of Strasbourg, 
      Strasbourg 67000, France.
LA  - eng
PT  - Journal Article
DEP - 20240222
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Single Molecule Imaging
MH  - *Nanoparticles/ultrastructure
MH  - *Quantum Dots
MH  - Luminescence
MH  - Immunoglobulin E
OTO - NOTNLM
OT  - high-affinity IgE receptor
OT  - nanoparticles
OT  - single particle tracking
OT  - single-molecule microscopy
OT  - upconversion
EDAT- 2024/02/22 18:41
MHDA- 2024/03/07 06:42
CRDT- 2024/02/22 12:03
PHST- 2024/03/07 06:42 [medline]
PHST- 2024/02/22 18:41 [pubmed]
PHST- 2024/02/22 12:03 [entrez]
AID - 10.1021/acsami.3c17116 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2024 Mar 6;16(9):11217-11227. doi: 
      10.1021/acsami.3c17116. Epub 2024 Feb 22.