PMID- 38386543
OWN - NLM
STAT- Publisher
LR  - 20240222
IS  - 1557-8542 (Electronic)
IS  - 1545-8547 (Linking)
DP  - 2024 Feb 20
TI  - Initial Steps of XY Sex Chromosome Differentiation in the Armored Catfish 
      Hypostomus albopunctatus (Siluriformes: Loricariidae) Revealed by Heterochromatin 
      Accumulation.
LID - 10.1089/zeb.2023.0100 [doi]
AB  - In fish species, heterochromatinization is one process that could trigger sex 
      chromosome differentiation. The present article describes a nascent XX/XY sex 
      chromosome system evidenced by heterochromatin accumulation and microsatellite 
      (GATA)(8) in Hypostomus albopunctatus from two populations of the Parana River 
      basin. The specimens of H. albopunctatus from the Campo and Bossi Rivers share 
      the same karyotype. The species exhibits 74 chromosomes (8m+14sm +16st +36a, 
      fundamental number = 112). The C-banding technique suggests male heterogamety in 
      H. albopunctatus, where the Y-chromosome is morphologically like the X-chromosome 
      but differs from it for having long arms that are entirely heterochromatic. 
      Double fluorescence in situ hybridization (FISH) with 18S and 5S rDNA probes 
      confirmed the Ag-nucleolus organizer region sites in a single pair for both 
      populations, and minor rDNA clusters showed interpopulational variation. FISH 
      with the microsatellite (GATA)(8) probe showed a dispersed pattern in the 
      karyotype, accumulating these sequences of sex chromosomes of both populations. 
      FISH with microsatellite (CGC)(10) probe showed interpopulational variation. The 
      absence of differentiated sex chromosomes in H. albopunctatus is described 
      previously, and a new variant is documented herein where XY chromosomes can be 
      seen in an early stage of differentiation.
FAU - Balini, Ligia Carla
AU  - Balini LC
AUID- ORCID: 0009-0008-1502-8408
AD  - Department of Biotechnology, Genetics, and Cell Biology, State University of 
      Maringa, Maringa, Parana, Brazil.
FAU - Fernandes, Carlos Alexandre
AU  - Fernandes CA
AUID- ORCID: 0000-0002-9905-1507
AD  - Department of Biotechnology, Genetics, and Cell Biology, State University of 
      Maringa, Maringa, Parana, Brazil.
AD  - Limnology, Ichthyology and Aquaculture Research Nucleus (NUPELIA), Biological 
      Sciences Center, State University of Maringa, Maringa, Parana, Brazil.
FAU - Portela-Castro, Ana Luiza de Brito
AU  - Portela-Castro ALB
AUID- ORCID: 0000-0002-0881-0345
AD  - Department of Biotechnology, Genetics, and Cell Biology, State University of 
      Maringa, Maringa, Parana, Brazil.
AD  - Limnology, Ichthyology and Aquaculture Research Nucleus (NUPELIA), Biological 
      Sciences Center, State University of Maringa, Maringa, Parana, Brazil.
FAU - Melo, Rafael Fernando de
AU  - Melo RF
AUID- ORCID: 0000-0002-6975-6346
AD  - Department of Biotechnology, Genetics, and Cell Biology, State University of 
      Maringa, Maringa, Parana, Brazil.
FAU - Zawadzki, Claudio Henrique
AU  - Zawadzki CH
AUID- ORCID: 0000-0002-5642-4908
AD  - Limnology, Ichthyology and Aquaculture Research Nucleus (NUPELIA), Biological 
      Sciences Center, State University of Maringa, Maringa, Parana, Brazil.
AD  - Department of Biology, State University of Maringa, Maringa, Parana, Brazil.
FAU - Borin-Carvalho, Luciana Andreia
AU  - Borin-Carvalho LA
AUID- ORCID: 0000-0003-1990-4803
AD  - Department of Biotechnology, Genetics, and Cell Biology, State University of 
      Maringa, Maringa, Parana, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20240220
PL  - United States
TA  - Zebrafish
JT  - Zebrafish
JID - 101225070
SB  - IM
OTO - NOTNLM
OT  - C-banding
OT  - FISH
OT  - early XY differentiation
OT  - homomorphic sex chromosomes
OT  - repetitive DNA
EDAT- 2024/02/22 18:41
MHDA- 2024/02/22 18:41
CRDT- 2024/02/22 12:33
PHST- 2024/02/22 18:41 [medline]
PHST- 2024/02/22 18:41 [pubmed]
PHST- 2024/02/22 12:33 [entrez]
AID - 10.1089/zeb.2023.0100 [doi]
PST - aheadofprint
SO  - Zebrafish. 2024 Feb 20. doi: 10.1089/zeb.2023.0100.