PMID- 38389384
OWN - NLM
STAT- Publisher
LR  - 20240223
IS  - 0191-2917 (Print)
IS  - 0191-2917 (Linking)
DP  - 2024 Feb 22
TI  - Introgression of an all-stage and broad-spectrum powdery mildew resistance gene 
      Pm3VS from Dasypyrum villosum chromosome 3V into wheat.
LID - 10.1094/PDIS-11-23-2495-RE [doi]
AB  - Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a serious 
      disease tothat threatens wheat production globally. It is imperative to explore 
      novel resistance genes in order to control this disease throughby developing and 
      planting resistant varieties. Here, we identified a wheat-Dasypyrum villosum 3V 
      (3D) disomic substitution line, NAU3815 (2n=42), with a high level of powdery 
      mildew resistance at both the seedling and adult-plant stages. Subsequently, 
      NAU3815 was used to generate recombination between chromosomes 3V and 3D. Through 
      genomic in situ hybridization (GISH), fluorescence in situ hybridization 
      (FISH)GISH/FISH and 3VS, 3VL-specific markers analysis, four introgression lines 
      were developed from the selfing progenies of 3V and 3D double monosomic line 
      NAU3816, which was derived from the F1 hybrids of NAU3815/NAU0686. There were 
      t3VS (3D) ditelosomic substitution line NAU3817, t3VL (3D) ditelosomic 
      substitution line NAU3818, homozygous T3DL.3VS translocation line NAU3819, and 
      homozygous T3DS.3VL translocation line NAU3820. Powdery mildew tests of these 
      lines confirmed the presence of an all-stage and broad-spectrum powdery mildew 
      resistance gene, Pm3VS, located on chromosome arm 3VS. When compared with the 
      recurrent parent NAU0686 plants, the T3DL.3VS translocation line NAU3819 showed 
      no obvious negative effect on yield-related traits. However, the introduction of 
      the T3DL.3VS translocated chromosome had a strong effect on reducing the 
      flag-leaf length. Consequently, the T3DL.3VS translocation line NAU3819 provides 
      a new germplasm in breeding for both resistance and plant architecture.
FAU - Hou, Fu
AU  - Hou F
AD  - Nanjing Agricultural University, 70578, College of Agronomy, Nanjing, Jiangsu, 
      China; 476949446@qq.com.
FAU - Chen, Heyu
AU  - Chen H
AD  - Nanjing Agricultural University, 70578, College of Agronomy, Nanjing, Jiangsu, 
      China; 2022101163@stu.njau.edu.cn.
FAU - Zhang, Ting
AU  - Zhang T
AD  - Nanjing Agricultural University, 70578, College of Agronomy, Nanjing, Jiangsu, 
      China; 2022801258@stu.njau.edu.cn.
FAU - Jin, Yinyu
AU  - Jin Y
AD  - Nanjing Agricultural University, 70578, College of Agronomy, Nanjing, Jiangsu, 
      China; 2022101166@stu.njau.edu.cn.
FAU - Kong, Lingna
AU  - Kong L
AD  - Nanjing Agricultural University, 70578, College of Agronomy, Nanjing, Jiangsu, 
      China; klnjj@njau.edu.cn.
FAU - Liu, Xiaoxue
AU  - Liu X
AD  - Nanjing Agricultural University, 70578, College of Agronomy, Nanjing, Jiangsu, 
      China; liuxiaoxue@njau.edu.cn.
FAU - Xing, Liping
AU  - Xing L
AD  - Nanjing Agricultural University, 70578, A318,the Science Building, Weigang No.1#, 
      Nanjing, Jiangsu Province, Nanjing, China, 210095; xingliping@njau.edu.cn.
FAU - Cao, Aizhong
AU  - Cao A
AD  - Nanjing Agricultural University, 70578, Nanjing, China; caoaz@njau.edu.cn.
FAU - Zhang, Ruiqi
AU  - Zhang R
AD  - Nanjing Agricultural University, 70578, College of Agronomy, Tongwei 6# road, 
      Nanjing City, Nanjing, Jiangsu, China, 210095; zrq@njau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20240222
PL  - United States
TA  - Plant Dis
JT  - Plant disease
JID - 9882809
SB  - IM
OTO - NOTNLM
OT  - Causal Agent
OT  - Crop Type
OT  - Disease management
OT  - Field crops
OT  - Fungi
OT  - Subject Areas
OT  - cereals and grains
OT  - cultivar/resistance
EDAT- 2024/02/23 06:42
MHDA- 2024/02/23 06:42
CRDT- 2024/02/23 02:21
PHST- 2024/02/23 06:42 [medline]
PHST- 2024/02/23 06:42 [pubmed]
PHST- 2024/02/23 02:21 [entrez]
AID - 10.1094/PDIS-11-23-2495-RE [doi]
PST - aheadofprint
SO  - Plant Dis. 2024 Feb 22. doi: 10.1094/PDIS-11-23-2495-RE.