PMID- 38391417 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240225 IS - 2310-2861 (Electronic) IS - 2310-2861 (Linking) VI - 10 IP - 2 DP - 2024 Jan 23 TI - In Vitro Release of Glycyrrhiza Glabra Extract by a Gel-Based Microneedle Patch for Psoriasis Treatment. LID - 10.3390/gels10020087 [doi] LID - 87 AB - Microneedle patches are attractive drug delivery systems that give hope for treating skin disorders. In this study, to first fabricate a chitosan-based low-cost microneedle patch (MNP) using a CO(2) laser cutter for in vitro purposes was tried and then the delivery and impact of Glycyrrhiza glabra extract (GgE) on the cell population by this microneedle was evaluated. Microscopic analysis, swelling, penetration, degradation, biocompatibility, and drug delivery were carried out to assess the patch's performance. DAPI staining and acridine orange (AO) staining were performed to evaluate cell numbers. Based on the results, the MNs were conical and sharp enough (diameter: 400-500 mum, height: 700-900 mum). They showed notable swelling (2 folds) during 5 min and good degradability during 30 min, which can be considered a burst release. The MNP showed no cytotoxicity against fibroblast cell line L929. It also demonstrated good potential for GgE delivery. The results from AO and DAPI staining approved the reduction in the cell population after GgE delivery. To sum up, the fabricated MNP can be a useful recommendation for lab-scale studies. In addition, a GgE-loaded MNP can be a good remedy for skin disorders in which cell proliferation needs to be controlled. FAU - Khorshidian, Ayeh AU - Khorshidian A AD - Department of Biomedical Engineering, TISSUEHUB Co., Tehran 1956854977, Iran. AD - Department of Tissue Engineering, TISSUEHUB Co., Tehran 1956854977, Iran. FAU - Sharifi, Niloufar AU - Sharifi N AD - Department of Tissue Engineering, TISSUEHUB Co., Tehran 1956854977, Iran. AD - School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450066, China. FAU - Choupani Kheirabadi, Fatemeh AU - Choupani Kheirabadi F AD - Department of Tissue Engineering, TISSUEHUB Co., Tehran 1956854977, Iran. AD - Department of Biomedical Engineering, Faculty of Engineering, Islamic Azad University, Tabriz 54911, Iran. FAU - Rezaei, Farnoushsadat AU - Rezaei F AD - Department of Chemical and Biomedical Engineering, University of Missouri, Columbia, MO 65211, USA. FAU - Sheikholeslami, Seyed Alireza AU - Sheikholeslami SA AD - Department of Tissue Engineering, TISSUEHUB Co., Tehran 1956854977, Iran. AD - Department of Chemical Engineering, Faculty of Engineering, Arak University, Arak 3848177584, Iran. FAU - Ariyannejad, Ayda AU - Ariyannejad A AD - Department of Tissue Engineering, TISSUEHUB Co., Tehran 1956854977, Iran. AD - Department of Marine Biology, Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran 1983969411, Iran. FAU - Esmaeili, Javad AU - Esmaeili J AUID- ORCID: 0000-0003-2853-9916 AD - Department of Tissue Engineering, TISSUEHUB Co., Tehran 1956854977, Iran. AD - Department of Chemical Engineering, Faculty of Engineering, Arak University, Arak 3848177584, Iran. AD - Tissue Engineering Hub (TEHUB), Universal Scientific Education and Research Network (USERN), Tehran 1956854977, Iran. FAU - Basati, Hojat AU - Basati H AD - Department of Tissue Engineering, TISSUEHUB Co., Tehran 1956854977, Iran. AD - Department of Chemical Engineering, Faculty of Engineering, Tehran University, Tehran 3584014179, Iran. FAU - Barati, Aboulfazl AU - Barati A AD - Center for Materials and Manufacturing Sciences, Department of Chemistry and Physics, Troy University, Troy, AL 36082, USA. LA - eng PT - Journal Article DEP - 20240123 PL - Switzerland TA - Gels JT - Gels (Basel, Switzerland) JID - 101696925 PMC - PMC10887857 OTO - NOTNLM OT - burst release OT - drug delivery OT - microneedle patch OT - psoriasis OT - skin disorder COIS- Authors Ayeh Khorshidian, Niloufar Sharifi, Fatemeh Choupani Kheirabadi, Seyed Alireza Sheikholeslami, Ayda Ariyannejad, Javad Esmaeili and Hojat Basati were employed by the company TISSUEHUB Co. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2024/02/23 12:44 MHDA- 2024/02/23 12:45 PMCR- 2024/01/23 CRDT- 2024/02/23 10:13 PHST- 2023/06/30 00:00 [received] PHST- 2023/10/01 00:00 [revised] PHST- 2024/01/17 00:00 [accepted] PHST- 2024/02/23 12:45 [medline] PHST- 2024/02/23 12:44 [pubmed] PHST- 2024/02/23 10:13 [entrez] PHST- 2024/01/23 00:00 [pmc-release] AID - gels10020087 [pii] AID - gels-10-00087 [pii] AID - 10.3390/gels10020087 [doi] PST - epublish SO - Gels. 2024 Jan 23;10(2):87. doi: 10.3390/gels10020087.