PMID- 38392977
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240225
IS  - 2218-1989 (Print)
IS  - 2218-1989 (Electronic)
IS  - 2218-1989 (Linking)
VI  - 14
IP  - 2
DP  - 2024 Jan 24
TI  - Proteomics-Based Investigation of Different Live Prey Administered to Freshwater 
      Dark Sleeper (Odontobutis potamophila): Examining the Effects on Glycolipids and 
      Energy Metabolism.
LID - 10.3390/metabo14020085 [doi]
LID - 85
AB  - Live prey is characterized by balanced rich nutrients and high palatability and 
      is widely used for the seedling cultivation of freshwater dark sleeper 
      (Odontobutis potamophila) larvae. In this study, we evaluated the effects of four 
      groups of paired feeding regimens (group C (Daphnia magna), group L (Limnodrilus 
      hoffmeisteri), group H (Hypophthalmichthys molitrix fry), and group M (mixed 
      groups C, L, and H)) on glycolipid and energy metabolism in O. potamophila 
      larvae. We observed that fatty acid synthase (FAS) and 
      sterol-regulatory-element-binding protein-1 (SREBP-1) mRNA levels were 
      significantly lower in group H when compared to mRNA levels in the other three 
      groups (p < 0.05) and that carnitine palmitoyltransferase 1alpha (CPT1-alpha) mRNA levels 
      were significantly lower in group L when compared to group M (p < 0.05). Relative 
      glucokinase (GK) expression levels were significantly lower in group M when 
      compared to the other three groups (p < 0.05). Using proteomics, we analyzed and 
      compared groups H and L and identified 457 differentially expressed proteins 
      (DEPs), of which 151 were significantly up-regulated and 306 were significantly 
      down-regulated. In the comparison of group M with groups C, L, and H, we found 
      significant enrichment in glycolytic processes, the endoplasmic reticulum lumen, 
      NAD binding, intermediate filaments, and nutrient reservoir activity. Our results 
      provide a theoretical guidance for bait selection during larvae cultivation 
      stages in carnivorous fish.
FAU - Zhou, Zihan
AU  - Zhou Z
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China.
FAU - Jiang, Qichen
AU  - Jiang Q
AD  - Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, 
      China.
AD  - Low-Temperature Germplasm Bank of Important Economic Fish (Freshwater Fisheries 
      Research Institute of Jiangsu Province) of Jiangsu Provincial Science and 
      Technology Resources (Agricultural Germplasm Resources) Coordination Service 
      Platform, Nanjing 210017, China.
FAU - Zheng, You
AU  - Zheng Y
AD  - Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, 
      China.
AD  - Low-Temperature Germplasm Bank of Important Economic Fish (Freshwater Fisheries 
      Research Institute of Jiangsu Province) of Jiangsu Provincial Science and 
      Technology Resources (Agricultural Germplasm Resources) Coordination Service 
      Platform, Nanjing 210017, China.
FAU - Hao, Chen
AU  - Hao C
AD  - Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, 
      China.
AD  - Low-Temperature Germplasm Bank of Important Economic Fish (Freshwater Fisheries 
      Research Institute of Jiangsu Province) of Jiangsu Provincial Science and 
      Technology Resources (Agricultural Germplasm Resources) Coordination Service 
      Platform, Nanjing 210017, China.
FAU - Ding, Shuyan
AU  - Ding S
AD  - Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, 
      China.
AD  - Low-Temperature Germplasm Bank of Important Economic Fish (Freshwater Fisheries 
      Research Institute of Jiangsu Province) of Jiangsu Provincial Science and 
      Technology Resources (Agricultural Germplasm Resources) Coordination Service 
      Platform, Nanjing 210017, China.
FAU - Guo, Mengya
AU  - Guo M
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China.
FAU - Zhao, Yunlong
AU  - Zhao Y
AD  - School of Life Science, East China Normal University, Shanghai 200241, China.
FAU - Liu, Guoxing
AU  - Liu G
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China.
AD  - Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, 
      China.
AD  - Low-Temperature Germplasm Bank of Important Economic Fish (Freshwater Fisheries 
      Research Institute of Jiangsu Province) of Jiangsu Provincial Science and 
      Technology Resources (Agricultural Germplasm Resources) Coordination Service 
      Platform, Nanjing 210017, China.
FAU - Miao, Shuyan
AU  - Miao S
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China.
LA  - eng
GR  - No. 31901094/Unit Project implementation Plan-Fisheries Ecology and Resources 
      monitoring in Jiangsu prov-ince, the Science Foundation of Jiangsu, China/
PT  - Journal Article
DEP - 20240124
PL  - Switzerland
TA  - Metabolites
JT  - Metabolites
JID - 101578790
PMC - PMC10890520
OTO - NOTNLM
OT  - FAS
OT  - GK
OT  - Odontobutis potamophila
OT  - glycolipid metabolism
OT  - proteomics
COIS- The authors declare no conflicts of interest.
EDAT- 2024/02/23 12:42
MHDA- 2024/02/23 12:43
PMCR- 2024/01/24
CRDT- 2024/02/23 10:26
PHST- 2023/11/08 00:00 [received]
PHST- 2023/12/26 00:00 [revised]
PHST- 2023/12/28 00:00 [accepted]
PHST- 2024/02/23 12:43 [medline]
PHST- 2024/02/23 12:42 [pubmed]
PHST- 2024/02/23 10:26 [entrez]
PHST- 2024/01/24 00:00 [pmc-release]
AID - metabo14020085 [pii]
AID - metabolites-14-00085 [pii]
AID - 10.3390/metabo14020085 [doi]
PST - epublish
SO  - Metabolites. 2024 Jan 24;14(2):85. doi: 10.3390/metabo14020085.