PMID- 38394389
OWN - NLM
STAT- Publisher
LR  - 20240305
IS  - 2192-2659 (Electronic)
IS  - 2192-2640 (Linking)
DP  - 2024 Feb 23
TI  - A 3D Bioprinted Human Neurovascular Unit Model of Glioblastoma Tumor Growth.
PG  - e2302831
LID - 10.1002/adhm.202302831 [doi]
AB  - A 3D bioprinted neurovascular unit (NVU) model is developed to study glioblastoma 
      (GBM) tumor growth in a brain-like microenvironment. The NVU model includes human 
      primary astrocytes, pericytes and brain microvascular endothelial cells, and 
      patient-derived glioblastoma cells (JHH-520) are used for this study. 
      Fluorescence reporters are used with confocal high content imaging to quantitate 
      real-time microvascular network formation and tumor growth. Extensive validation 
      of the NVU-GBM model includes immunostaining for brain relevant cellular markers 
      and extracellular matrix components; single cell RNA sequencing (scRNAseq) to 
      establish physiologically relevant transcriptomics changes; and secretion of NVU 
      and GBM-relevant cytokines. The scRNAseq reveals changes in gene expression and 
      cytokines secretion associated with wound healing/angiogenesis, including the 
      appearance of an endothelial mesenchymal transition cell population. The NVU-GBM 
      model is used to test 18 chemotherapeutics and anti-cancer drugs to assess the 
      pharmacological relevance of the model and robustness for high throughput 
      screening.
CI  - Published 2024. This article is a U.S. Government work and is in the public 
      domain in the USA. Advanced Healthcare Materials published by Wiley-VCH GmbH.
FAU - Tung, Yen-Ting
AU  - Tung YT
AD  - National Center for Advancing Translational Sciences (NCATS), National Institutes 
      of Health (NIH), Rockville, MD, 20850, USA.
FAU - Chen, Yu-Chi
AU  - Chen YC
AD  - National Center for Advancing Translational Sciences (NCATS), National Institutes 
      of Health (NIH), Rockville, MD, 20850, USA.
FAU - Derr, Kristy
AU  - Derr K
AD  - National Center for Advancing Translational Sciences (NCATS), National Institutes 
      of Health (NIH), Rockville, MD, 20850, USA.
FAU - Wilson, Kelli
AU  - Wilson K
AD  - National Center for Advancing Translational Sciences (NCATS), National Institutes 
      of Health (NIH), Rockville, MD, 20850, USA.
FAU - Song, Min Jae
AU  - Song MJ
AUID- ORCID: 0000-0003-1744-0806
AD  - National Center for Advancing Translational Sciences (NCATS), National Institutes 
      of Health (NIH), Rockville, MD, 20850, USA.
FAU - Ferrer, Marc
AU  - Ferrer M
AD  - National Center for Advancing Translational Sciences (NCATS), National Institutes 
      of Health (NIH), Rockville, MD, 20850, USA.
LA  - eng
PT  - Journal Article
DEP - 20240223
PL  - Germany
TA  - Adv Healthc Mater
JT  - Advanced healthcare materials
JID - 101581613
SB  - IM
OTO - NOTNLM
OT  - 3D Bioprinting
OT  - glioblastomas
OT  - high-throughput screening
OT  - neurovascular unit
OT  - transcriptomics
EDAT- 2024/02/23 18:42
MHDA- 2024/02/23 18:42
CRDT- 2024/02/23 15:12
PHST- 2024/01/29 00:00 [revised]
PHST- 2023/10/01 00:00 [received]
PHST- 2024/02/23 18:42 [pubmed]
PHST- 2024/02/23 18:42 [medline]
PHST- 2024/02/23 15:12 [entrez]
AID - 10.1002/adhm.202302831 [doi]
PST - aheadofprint
SO  - Adv Healthc Mater. 2024 Feb 23:e2302831. doi: 10.1002/adhm.202302831.