PMID- 38394647
OWN - NLM
STAT- Publisher
LR  - 20240410
IS  - 1480-3321 (Electronic)
IS  - 0831-2796 (Linking)
DP  - 2024 Feb 23
TI  - Development and application of whole-chromosome painting of chromosomes 7A and 8A 
      of Arachis duranensis based on chromosome-specific single-copy oligonucleotides.
LID - 10.1139/gen-2023-0116 [doi]
AB  - For peanut, the lack of stable cytological markers is a barrier to tracking 
      specific chromosomes, elucidating the genetic relationships between genomes and 
      identifying chromosomal variations. Chromosome mapping using single-copy 
      oligonucleotide (oligo) probe libraries has unique advantages for identifying 
      homologous chromosomes and chromosomal rearrangements. In this study, we 
      developed two whole-chromosome single-copy oligo probe libraries, LS-7A and 
      LS-8A, based on the reference genome sequences of chromosomes 7A and 8A of 
      Arachis duranensis. Fluorescence in situ hybridization (FISH) analysis confirmed 
      that the libraries could specifically paint chromosomes 7 and 8. In addition, 
      sequential FISH and electronic localization of LS-7A and LS-8A in A. duranensis 
      (AA) and A. ipaensis (BB) showed that chromosomes 7A and 8A contained 
      translocations and inversions relative to chromosomes 7B and 8B. Analysis of the 
      chromosomes of wild Arachis species using LS-8A confirmed that this library could 
      accurately and effectively identify A genome species. Finally, LS-7A and LS-8A 
      were used to paint the chromosomes of interspecific hybrids and their progenies, 
      which verified the authenticity of the interspecific hybrids and identified a 
      disomic addition line. This study provides a model for developing specific oligo 
      probes to identify the structural variations of other chromosomes in Arachis and 
      demonstrates the practical utility of LS-7A and LS-8A.
FAU - Li, Chenyu
AU  - Li C
AUID- ORCID: 0009-0009-7492-2212
AD  - College of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, 
      China. RINGGOLD: 200644
FAU - Fu, Liuyang
AU  - Fu L
AD  - College of Life Science, Zhengzhou University, Zhengzhou, Henan 450001, China. 
      RINGGOLD: 12636
FAU - Wang, Qian
AU  - Wang Q
AD  - College of Life Science, Zhengzhou University, Zhengzhou, Henan 450001, China. 
      RINGGOLD: 12636
AD  - The Shennong Laboratory/Nation Industrial Innovation Centre for Bio-Breeding/Key 
      Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan 
      Provincial Key Laboratory for Oil Crops Improvement/Institute of Crop Molecular 
      Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China. 
      RINGGOLD: 74728
FAU - Liu, Hua
AU  - Liu H
AD  - The Shennong Laboratory/Nation Industrial Innovation Centre for Bio-Breeding/Key 
      Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan 
      Provincial Key Laboratory for Oil Crops Improvement/Institute of Crop Molecular 
      Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China. 
      RINGGOLD: 74728
FAU - Chen, Guoquan
AU  - Chen G
AD  - College of Life Science, Zhengzhou University, Zhengzhou, Henan 450001, China. 
      RINGGOLD: 12636
AD  - The Shennong Laboratory/Nation Industrial Innovation Centre for Bio-Breeding/Key 
      Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan 
      Provincial Key Laboratory for Oil Crops Improvement/Institute of Crop Molecular 
      Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China. 
      RINGGOLD: 74728
FAU - Qi, Feiyan
AU  - Qi F
AD  - The Shennong Laboratory/Nation Industrial Innovation Centre for Bio-Breeding/Key 
      Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan 
      Provincial Key Laboratory for Oil Crops Improvement/Institute of Crop Molecular 
      Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China. 
      RINGGOLD: 74728
FAU - Zhang, Maoning
AU  - Zhang M
AD  - College of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, 
      China. RINGGOLD: 200644
FAU - Jia, Yaoguang
AU  - Jia Y
AD  - College of Life Science, Zhengzhou University, Zhengzhou, Henan 450001, China. 
      RINGGOLD: 12636
FAU - Li, Xiaona
AU  - Li X
AD  - College of Life Science, Zhengzhou University, Zhengzhou, Henan 450001, China. 
      RINGGOLD: 12636
AD  - The Shennong Laboratory/Nation Industrial Innovation Centre for Bio-Breeding/Key 
      Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan 
      Provincial Key Laboratory for Oil Crops Improvement/Institute of Crop Molecular 
      Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China. 
      RINGGOLD: 74728
FAU - Huang, Bingyan
AU  - Huang B
AD  - The Shennong Laboratory/Nation Industrial Innovation Centre for Bio-Breeding/Key 
      Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan 
      Provincial Key Laboratory for Oil Crops Improvement/Institute of Crop Molecular 
      Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China. 
      RINGGOLD: 74728
FAU - Dong, Wenzhao
AU  - Dong W
AD  - The Shennong Laboratory/Nation Industrial Innovation Centre for Bio-Breeding/Key 
      Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan 
      Provincial Key Laboratory for Oil Crops Improvement/Institute of Crop Molecular 
      Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China. 
      RINGGOLD: 74728
FAU - Du, Pei
AU  - Du P
AUID- ORCID: 0000-0001-5646-5972
AD  - The Shennong Laboratory/Nation Industrial Innovation Centre for Bio-Breeding/Key 
      Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan 
      Provincial Key Laboratory for Oil Crops Improvement/Institute of Crop Molecular 
      Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China. 
      RINGGOLD: 74728
FAU - Zhang, Xinyou
AU  - Zhang X
AUID- ORCID: 0000-0002-1942-997X
AD  - College of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, 
      China. RINGGOLD: 200644
AD  - The Shennong Laboratory/Nation Industrial Innovation Centre for Bio-Breeding/Key 
      Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan 
      Provincial Key Laboratory for Oil Crops Improvement/Institute of Crop Molecular 
      Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China. 
      RINGGOLD: 74728
LA  - eng
PT  - Journal Article
DEP - 20240223
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
SB  - IM
OTO - NOTNLM
OT  - Arachis species
OT  - chromosomal variation
OT  - chromosome painting
OT  - interspecific hybridization
OT  - single-copy oligonucleotide
COIS- The authors declare that they have no competing interests.
EDAT- 2024/02/23 18:42
MHDA- 2024/02/23 18:42
CRDT- 2024/02/23 16:42
PHST- 2024/02/23 18:42 [pubmed]
PHST- 2024/02/23 18:42 [medline]
PHST- 2024/02/23 16:42 [entrez]
AID - 10.1139/gen-2023-0116 [doi]
PST - aheadofprint
SO  - Genome. 2024 Feb 23. doi: 10.1139/gen-2023-0116.