PMID- 38396788
OWN - NLM
STAT- MEDLINE
DCOM- 20240226
LR  - 20240313
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 25
IP  - 4
DP  - 2024 Feb 9
TI  - The Differential Effect of a Shortage of Thyroid Hormone Compared with Knockout 
      of Thyroid Hormone Transporters Mct8 and Mct10 on Murine Macrophage Polarization.
LID - 10.3390/ijms25042111 [doi]
LID - 2111
AB  - Innate immune cells, including macrophages, are functionally affected by thyroid 
      hormone (TH). Macrophages can undergo phenotypical alterations, shifting between 
      proinflammatory (M1) and immunomodulatory (M2) profiles. Cellular TH 
      concentrations are, among others, determined by TH transporters. To study the 
      effect of TH and TH transporters on macrophage polarization, specific 
      proinflammatory and immunomodulatory markers were analyzed in bone marrow-derived 
      macrophages (BMDMs) depleted of triiodothyronine (T3) and BMDMs with a knockout 
      (KO) of Mct8 and Mct10 and a double KO (dKO) of Mct10/Mct8. Our findings show 
      that T3 is important for M1 polarization, while a lack of T3 stimulates M2 
      polarization. Mct8 KO BMDMs are unaffected in their T3 responsiveness, but 
      exhibit slight alterations in M2 polarization, while Mct10 KO BMDMs show reduced 
      T3 responsiveness, but unaltered polarization markers. KO of both the Mct8 and 
      Mct10 transporters decreased T3 availability and, contrary to the T3-depleted 
      BMDMs, showed partially increased M1 markers and unaltered M2 markers. These data 
      suggest a role for TH transporters besides transport of TH in BMDMs. This study 
      highlights the complex role of TH transporters in macrophages and provides a new 
      angle on the interaction between the endocrine and immune systems.
FAU - Hoen, Esmee
AU  - Hoen E
AD  - Endocrine Laboratory, Department of Laboratory Medicine, Amsterdam 
      Gastroenterology Endocrinology & Metabolism (AGEM), Amsterdam UMC, University of 
      Amsterdam, 1105 AZ Amsterdam, The Netherlands.
FAU - Goossens, Franka M
AU  - Goossens FM
AD  - Endocrine Laboratory, Department of Laboratory Medicine, Amsterdam 
      Gastroenterology Endocrinology & Metabolism (AGEM), Amsterdam UMC, University of 
      Amsterdam, 1105 AZ Amsterdam, The Netherlands.
FAU - Falize, Kim
AU  - Falize K
AD  - Endocrine Laboratory, Department of Laboratory Medicine, Amsterdam 
      Gastroenterology Endocrinology & Metabolism (AGEM), Amsterdam UMC, University of 
      Amsterdam, 1105 AZ Amsterdam, The Netherlands.
FAU - Mayerl, Steffen
AU  - Mayerl S
AD  - Department of Endocrinology, Diabetes & Metabolism, University Duisburg-Essen, 
      47057 Essen, Germany.
FAU - van der Spek, Anne H
AU  - van der Spek AH
AD  - Department of Endocrinology and Metabolism, Amsterdam Gastroenterology 
      Endocrinology & Metabolism (AGEM), Amsterdam UMC, University of Amsterdam, 1105 
      AZ Amsterdam, The Netherlands.
FAU - Boelen, Anita
AU  - Boelen A
AUID- ORCID: 0000-0002-4994-2918
AD  - Endocrine Laboratory, Department of Laboratory Medicine, Amsterdam 
      Gastroenterology Endocrinology & Metabolism (AGEM), Amsterdam UMC, University of 
      Amsterdam, 1105 AZ Amsterdam, The Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20240209
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Monocarboxylic Acid Transporters)
RN  - 0 (Symporters)
RN  - 0 (Thyroid Hormones)
RN  - 06LU7C9H1V (Triiodothyronine)
RN  - 0 (Slc16a2 protein, mouse)
RN  - 0 (Slc16a10 protein, mouse)
RN  - 0 (Amino Acid Transport Systems, Neutral)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Macrophages/metabolism
MH  - Monocarboxylic Acid Transporters/genetics
MH  - *Symporters/genetics
MH  - *Thyroid Hormones/metabolism/pharmacology
MH  - Triiodothyronine/pharmacology
MH  - Amino Acid Transport Systems, Neutral/genetics/metabolism
PMC - PMC10889717
OTO - NOTNLM
OT  - Mct10
OT  - Mct8
OT  - T3
OT  - bone marrow-derived macrophages
OT  - innate immune system
OT  - thyroid hormone
OT  - thyroid hormone transporters
COIS- The authors declare no conflicts of interest. The funders had no role in the 
      design of the study; in the collection, analyses or interpretation of data; in 
      the writing of the manuscript; or in the decision to publish the results.
EDAT- 2024/02/24 11:45
MHDA- 2024/02/26 06:44
PMCR- 2024/02/09
CRDT- 2024/02/24 01:03
PHST- 2023/12/22 00:00 [received]
PHST- 2024/01/24 00:00 [revised]
PHST- 2024/02/06 00:00 [accepted]
PHST- 2024/02/26 06:44 [medline]
PHST- 2024/02/24 11:45 [pubmed]
PHST- 2024/02/24 01:03 [entrez]
PHST- 2024/02/09 00:00 [pmc-release]
AID - ijms25042111 [pii]
AID - ijms-25-02111 [pii]
AID - 10.3390/ijms25042111 [doi]
PST - epublish
SO  - Int J Mol Sci. 2024 Feb 9;25(4):2111. doi: 10.3390/ijms25042111.