PMID- 38397449
OWN - NLM
STAT- MEDLINE
DCOM- 20240226
LR  - 20240228
IS  - 2218-273X (Electronic)
IS  - 2218-273X (Linking)
VI  - 14
IP  - 2
DP  - 2024 Feb 11
TI  - The Association of Vitamin D Receptor Gene Polymorphisms with Vitamin D, Total 
      IgE, and Blood Eosinophils in Patients with Atopy.
LID - 10.3390/biom14020212 [doi]
LID - 212
AB  - BACKGROUND: In order to improve the control of atopic diseases, it is important 
      to clarify the pathogenesis of atopy and identify its various triggers. Single 
      nucleotide polymorphisms (SNPs) of the vitamin D receptor gene (VDR) may impact 
      atopy. The aim of this study was to investigate the possible associations between 
      VDR SNPs and vitamin D, total IgE, and eosinophils in atopy. METHODS: In total, 
      203 adults, including 122 patients with atopic diseases (45 with atopic 
      dermatitis, 77 with allergic asthma) and 81 healthy controls, were involved in 
      the study. The blood eosinophil count was determined with an automated hematology 
      analyzer. Vitamin D and total immunoglobulin E (IgE) levels were evaluated using 
      the ELISA method. Polymorphisms in the VDR gene were analyzed with real-time PCR 
      using TaqMan probes. RESULTS: We analyzed six VDR single nucleotide polymorphisms 
      and found a significant association between VDR rs731236 GG genotype and normal 
      vitamin D levels in atopic patients and healthy subjects (OR 11.33; 95% CI: 
      1.049-122.388 and OR 4.04; 95% CI: 1.117-14.588, respectively, p < 0.05). 
      Additionally, the study results revealed a significant relationship between the 
      VDR rs2228570 GG genotype and normal vitamin D levels in patients with atopy and 
      healthy subjects (OR 3.80; 95% CI: 1.190-12.134 and OR 2.09; 95% CI: 1.044-4.194, 
      respectively, p < 0.05). The rs2228570 allele A was associated with decreased 
      vitamin D levels in patients with atopy and healthy subjects (OR 0.28; 95% CI: 
      0.098-0.804 and OR 0.229; 95% CI: 0.069-0.761, respectively, p < 0.05). The VDR 
      rs3847987 genotypes AA and AC were significantly associated with normal vitamin D 
      levels in healthy subjects (OR 35.99; 95% CI: 6.401-202.446 and OR 4.72; 95% CI: 
      1.489-15.007, respectively, p < 0.05). In addition, a decreased amount of vitamin 
      D was associated with atopic diseases such as atopic dermatitis and allergic 
      asthma (OR 0.49; 95% CI: 0.439-1.308 and OR 0.58; 95% CI: 0.372-0.908, 
      respectively, p < 0.05). The rs11168293 allele T was associated with the normal 
      range of total IgE in atopy (OR 2.366; 95% CI: 1.133-5.027; p < 0.05). 
      Significant associations were found between VDR rs731263 allele G, rs11168293 
      allele G, and increased blood eosinophil levels in patients with atopy (OR 0.319; 
      95% CI: 0.163-0.934 and OR 0.323; 95% CI: 0.112-0.935, respectively, p < 0.05). 
      CONCLUSIONS: A decreased vitamin D level showed a significant relationship with 
      atopic diseases (atopic dermatitis and allergic asthma). The association between 
      the VDR gene polymorphisms rs2228570, rs731236, and rs11168293 and vitamin D, 
      total IgE, and blood eosinophils in patients with atopy suggested that VDR 
      polymorphisms and the vitamin D level should be considered when examining the 
      factors associated with atopy.
FAU - Bastyte, Daina
AU  - Bastyte D
AD  - Department of Immunology and Allergology, Lithuanian University of Health 
      Sciences, LT-50161 Kaunas, Lithuania.
AD  - Laboratory of Immunology, Department of Immunology and Allergology, Lithuanian 
      University of Health Sciences, LT-50161 Kaunas, Lithuania.
FAU - Tamasauskiene, Laura
AU  - Tamasauskiene L
AD  - Department of Immunology and Allergology, Lithuanian University of Health 
      Sciences, LT-50161 Kaunas, Lithuania.
AD  - Laboratory of Immunology, Department of Immunology and Allergology, Lithuanian 
      University of Health Sciences, LT-50161 Kaunas, Lithuania.
FAU - Stakaitiene, Ieva
AU  - Stakaitiene I
AUID- ORCID: 0000-0003-2739-8576
AD  - Department of Genetics and Molecular Medicine, Lithuanian University of Health 
      Sciences, LT-50161 Kaunas, Lithuania.
FAU - Ugenskiene, Rasa
AU  - Ugenskiene R
AD  - Department of Genetics and Molecular Medicine, Lithuanian University of Health 
      Sciences, LT-50161 Kaunas, Lithuania.
FAU - Gradauskiene Sitkauskiene, Brigita
AU  - Gradauskiene Sitkauskiene B
AD  - Department of Immunology and Allergology, Lithuanian University of Health 
      Sciences, LT-50161 Kaunas, Lithuania.
LA  - eng
GR  - P-LLT-20-4/Research Council of Lithuania from the Mutual Funds of the 
      Latvia-Lithuania-Taiwan Cooperation Project/
PT  - Journal Article
DEP - 20240211
PL  - Switzerland
TA  - Biomolecules
JT  - Biomolecules
JID - 101596414
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 0 (Receptors, Calcitriol)
RN  - 1406-16-2 (Vitamin D)
RN  - 0 (VDR protein, human)
SB  - IM
MH  - Adult
MH  - Humans
MH  - *Asthma/genetics
MH  - Case-Control Studies
MH  - *Dermatitis, Atopic/genetics
MH  - Eosinophils
MH  - Gene Frequency
MH  - Genetic Predisposition to Disease
MH  - Immunoglobulin E
MH  - Polymorphism, Single Nucleotide
MH  - Receptors, Calcitriol/genetics
MH  - Vitamin D
PMC - PMC10887061
OTO - NOTNLM
OT  - asthma
OT  - atopic dermatitis
OT  - atopy
OT  - single nucleotide polymorphism
OT  - vitamin D
OT  - vitamin D deficiency
OT  - vitamin D receptor
COIS- The authors declare no conflicts of interest.
EDAT- 2024/02/24 11:45
MHDA- 2024/02/26 06:45
PMCR- 2024/02/11
CRDT- 2024/02/24 01:07
PHST- 2023/12/15 00:00 [received]
PHST- 2024/01/24 00:00 [revised]
PHST- 2024/02/09 00:00 [accepted]
PHST- 2024/02/26 06:45 [medline]
PHST- 2024/02/24 11:45 [pubmed]
PHST- 2024/02/24 01:07 [entrez]
PHST- 2024/02/11 00:00 [pmc-release]
AID - biom14020212 [pii]
AID - biomolecules-14-00212 [pii]
AID - 10.3390/biom14020212 [doi]
PST - epublish
SO  - Biomolecules. 2024 Feb 11;14(2):212. doi: 10.3390/biom14020212.