PMID- 38401119
OWN - NLM
STAT- MEDLINE
DCOM- 20240401
LR  - 20240504
IS  - 2211-1247 (Electronic)
VI  - 43
IP  - 3
DP  - 2024 Mar 26
TI  - A transcription factor atlas of stem cell fate in planarians.
PG  - 113843
LID - S2211-1247(24)00171-2 [pii]
LID - 10.1016/j.celrep.2024.113843 [doi]
AB  - Whole-body regeneration requires the ability to produce the full repertoire of 
      adult cell types. The planarian Schmidtea mediterranea contains over 125 cell 
      types, which can be regenerated from a stem cell population called neoblasts. 
      Neoblast fate choice can be regulated by the expression of fate-specific 
      transcription factors (FSTFs). How fate choices are made and distributed across 
      neoblasts versus their post-mitotic progeny remains unclear. We used single-cell 
      RNA sequencing to systematically map fate choices made in S/G(2)/M neoblasts and, 
      separately, in their post-mitotic progeny that serve as progenitors for all adult 
      cell types. We defined transcription factor expression signatures associated with 
      all detected fates, identifying numerous new progenitor classes and FSTFs that 
      regulate them. Our work generates an atlas of stem cell fates with associated 
      transcription factor signatures for most cell types in a complete adult organism.
CI  - Copyright (c) 2024 The Author(s). Published by Elsevier Inc. All rights reserved.
FAU - King, Hunter O
AU  - King HO
AD  - Whitehead Institute for Biomedical Research, Cambridge, MA, USA; Department of 
      Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, 
      MA, USA.
FAU - Owusu-Boaitey, Kwadwo E
AU  - Owusu-Boaitey KE
AD  - Whitehead Institute for Biomedical Research, Cambridge, MA, USA; Department of 
      Biology, Massachusetts Institute of Technology, Cambridge, MA, USA; Harvard/MIT 
      MD-PhD Program, Harvard Medical School, Boston, MA, USA.
FAU - Fincher, Christopher T
AU  - Fincher CT
AD  - Whitehead Institute for Biomedical Research, Cambridge, MA, USA; Department of 
      Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
FAU - Reddien, Peter W
AU  - Reddien PW
AD  - Howard Hughes Medical Institute, Chevy Chase, MD, USA; Whitehead Institute for 
      Biomedical Research, Cambridge, MA, USA; Department of Biology, Massachusetts 
      Institute of Technology, Cambridge, MA, USA. Electronic address: 
      reddien@wi.mit.edu.
LA  - eng
GR  - R35 GM145345/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20240223
PL  - United States
TA  - Cell Rep
JT  - Cell reports
JID - 101573691
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - *Transcription Factors/genetics/metabolism
MH  - *Planarians/metabolism
MH  - Stem Cells/metabolism
MH  - Cell Differentiation
MH  - Gene Expression Regulation
OTO - NOTNLM
OT  - CP: Stem cell research
OT  - cell fate
OT  - neoblasts
OT  - planarians
OT  - progenitor
OT  - regeneration
OT  - stem cells
OT  - transcription factor
COIS- Declaration of interests The authors declare no competing interests.
EDAT- 2024/02/24 21:44
MHDA- 2024/04/01 06:43
CRDT- 2024/02/24 12:35
PHST- 2023/04/05 00:00 [received]
PHST- 2023/12/05 00:00 [revised]
PHST- 2024/02/06 00:00 [accepted]
PHST- 2024/04/01 06:43 [medline]
PHST- 2024/02/24 21:44 [pubmed]
PHST- 2024/02/24 12:35 [entrez]
AID - S2211-1247(24)00171-2 [pii]
AID - 10.1016/j.celrep.2024.113843 [doi]
PST - ppublish
SO  - Cell Rep. 2024 Mar 26;43(3):113843. doi: 10.1016/j.celrep.2024.113843. Epub 2024 
      Feb 23.