PMID- 38401691
OWN - NLM
STAT- MEDLINE
DCOM- 20240427
LR  - 20240427
IS  - 1873-4847 (Electronic)
IS  - 0955-2863 (Linking)
VI  - 128
DP  - 2024 Jun
TI  - Nutrient composition of different energy-restricted diets determines plasma 
      endocannabinoid profiles and adipose tissue DAGL-alpha expression; a 12-week 
      randomized controlled trial in subjects with abdominal obesity.
PG  - 109605
LID - S0955-2863(24)00038-X [pii]
LID - 10.1016/j.jnutbio.2024.109605 [doi]
AB  - The endocannabinoid system (ECS) is dysregulated during obesity and metabolic 
      disorders. Weight loss favours the re-establishment of ECS homeostatic 
      conditions, but also the fatty acid composition of the diet can modulate 
      endocannabinoid profiles. However, the combined impact of nutrient quality and 
      energy restriction on the ECS remains unclear. In this 12 weeks randomized 
      controlled trial, men and women (40-70 years) with obesity (BMI: 31.3 +/- 3.5 kg/ 
      m(2)) followed either a low nutrient quality 25% energy-restricted (ER) diet 
      (n=39) high in saturated fats and fructose, or a high nutrient quality ER diet 
      (n=34) amongst others enriched in n-3 polyunsaturated fatty acids (PUFAs) or kept 
      their habitual diet (controls). Profiles of plasma- and adipose 
      N-acylethanolamines and mono-acyl glycerol esters were quantified using LC-MS/MS. 
      Gene expression of ECS-related enzymes and receptors was determined in adipose 
      tissue. Measurements were performed under fasting conditions before and after 12 
      weeks. Our results showed that plasma level of the DHA-derived compound 
      docosahexaenoylethanolamide (DHEA) was decreased in the low nutrient quality ER 
      diet (P<0.001) compared with the high nutrient quality ER diet, whereas 
      anandamide (AEA) and arachidonoylglycerol (2-AG) levels were unaltered. However, 
      adipose tissue gene expression of the 2-AG synthesizing enzyme diacylglycerol 
      lipase alpha (DAGL-alpha) was increased following the low nutrient quality ER diet 
      (P<.009) and differed upon intervention with both other diets. Concluding, 
      nutrient quality of the diet affects N-acylethanolamine profiles and gene 
      expression of ECS-related enzymes and receptors even under conditions of high 
      energy restriction in abdominally obese humans. ClinicalTrials.gov NCT02194504.
CI  - Copyright (c) 2024 The Author(s). Published by Elsevier Inc. All rights reserved.
FAU - Wang, Ya
AU  - Wang Y
AD  - Division of Human Nutrition and Health, Wageningen University, Wageningen, The 
      Netherlands; The Laboratory of Food Chemistry, Wageningen University, Wageningen, 
      The Netherlands.
FAU - Balvers, Michiel G J
AU  - Balvers MGJ
AD  - Division of Human Nutrition and Health, Wageningen University, Wageningen, The 
      Netherlands.
FAU - Esser, Diederik
AU  - Esser D
AD  - Division of Human Nutrition and Health, Wageningen University, Wageningen, The 
      Netherlands.
FAU - Schutte, Sophie
AU  - Schutte S
AD  - Division of Human Nutrition and Health, Wageningen University, Wageningen, The 
      Netherlands.
FAU - Vincken, Jean-Paul
AU  - Vincken JP
AD  - The Laboratory of Food Chemistry, Wageningen University, Wageningen, The 
      Netherlands.
FAU - Afman, Lydia A
AU  - Afman LA
AD  - Division of Human Nutrition and Health, Wageningen University, Wageningen, The 
      Netherlands.
FAU - Witkamp, Renger F
AU  - Witkamp RF
AD  - Division of Human Nutrition and Health, Wageningen University, Wageningen, The 
      Netherlands.
FAU - Meijerink, Jocelijn
AU  - Meijerink J
AD  - Division of Human Nutrition and Health, Wageningen University, Wageningen, The 
      Netherlands. Electronic address: jocelijn.meijerink@wur.nl.
LA  - eng
SI  - ClinicalTrials.gov/NCT02194504
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20240223
PL  - United States
TA  - J Nutr Biochem
JT  - The Journal of nutritional biochemistry
JID - 9010081
RN  - 0 (Endocannabinoids)
RN  - EC 3.1.1.34 (Lipoprotein Lipase)
RN  - 0 (N-acylethanolamines)
RN  - 0 (Ethanolamines)
SB  - IM
MH  - Humans
MH  - *Endocannabinoids/metabolism/blood
MH  - Middle Aged
MH  - Male
MH  - Female
MH  - Adult
MH  - Aged
MH  - *Caloric Restriction
MH  - *Adipose Tissue/metabolism
MH  - *Obesity, Abdominal/diet therapy/metabolism/blood
MH  - *Lipoprotein Lipase/metabolism
MH  - Ethanolamines/metabolism
MH  - Nutrients/metabolism
OTO - NOTNLM
OT  - CB(1)
OT  - Docosahexaenoylethanolamide (DHEA)
OT  - Endocannabinoid system
OT  - Metabolic disorders
OT  - N-acylethanolamines
OT  - n-3 PUFA
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2024/02/25 00:42
MHDA- 2024/04/28 07:24
CRDT- 2024/02/24 19:14
PHST- 2023/10/19 00:00 [received]
PHST- 2024/02/14 00:00 [revised]
PHST- 2024/02/19 00:00 [accepted]
PHST- 2024/04/28 07:24 [medline]
PHST- 2024/02/25 00:42 [pubmed]
PHST- 2024/02/24 19:14 [entrez]
AID - S0955-2863(24)00038-X [pii]
AID - 10.1016/j.jnutbio.2024.109605 [doi]
PST - ppublish
SO  - J Nutr Biochem. 2024 Jun;128:109605. doi: 10.1016/j.jnutbio.2024.109605. Epub 
      2024 Feb 23.