PMID- 38407521
OWN - NLM
STAT- Publisher
LR  - 20240313
IS  - 1526-4998 (Electronic)
IS  - 1526-498X (Linking)
DP  - 2024 Feb 26
TI  - Overexpression of miR-927-5p suppresses stalky expression and negatively reduces 
      the spermatid production in Zeugodacus cucurbitae.
LID - 10.1002/ps.8044 [doi]
AB  - BACKGROUND: The melon fly, Zeugodacus cucurbitae Coquillett, is one of the major 
      pests attacking Cucurbitaceae crops. Identifying critical genes or proteins 
      regulating fertility is essential for sustainable pest control and a research 
      hotspot in insect physiology. MicroRNAs (miRNAs) are short RNAs that do not 
      directly participate in protein translation, but instead function in 
      post-transcriptional regulation of gene expression involved in male fertility. 
      RESULTS: We found that miR-927-5p is highly expressed in the testes and 
      investigated its function in spermatogenesis in Z. cucurbitae. Fluorescence in 
      situ hybridization (FISH) showed miR-927-5p in the transformation and maturation 
      region of the testis, and overexpression of miR-927-5p reduced the number of 
      sperms by 53%. In continuation, we predicted 12 target genes of miR-927-5p using 
      bioinformatics combined with transcriptome sequencing data, and found that 
      miR-927-5p targets the new gene Stalky in insects, which was validated by 
      quantitative real-time PCR, RNA pull-down and dual luciferase reporter assays. 
      FISH also confirmed the co-localization of miR-927-5p and the transcript Stalky_1 
      in the testis. Moreover, silencing of Stalky_1 by RNA interference reduced the 
      number of sperms by 32% and reduced sperm viability by 39% in physiologically 
      mature male adults. Meanwhile, the silencing of Stalky_1 also resulted in low 
      hatchability. CONCLUSION: Our work not only presents a new, so far unreported 
      mechanism regulating spermatogenesis by miR-927-5p targeting a new unknown 
      target, Stalky, which is providing new knowledge on the regulatory network of 
      insect spermatogenesis, but also lays a foundation for the development of SIT 
      against important tephritid fly pests. (c) 2024 Society of Chemical Industry.
CI  - (c) 2024 Society of Chemical Industry.
FAU - Han, Hong-Liang
AU  - Han HL
AD  - Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of 
      Plant Protection, Southwest University, Chongqing, China.
AD  - International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, 
      Academy of Agricultural Sciences, Southwest University, Chongqing, China.
AD  - Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze 
      River (Ministry of Education), Southwest University, Chongqing, China.
FAU - Li, Jing-Ming
AU  - Li JM
AD  - Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of 
      Plant Protection, Southwest University, Chongqing, China.
AD  - International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, 
      Academy of Agricultural Sciences, Southwest University, Chongqing, China.
AD  - Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze 
      River (Ministry of Education), Southwest University, Chongqing, China.
FAU - Chen, Dong
AU  - Chen D
AD  - Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of 
      Plant Protection, Southwest University, Chongqing, China.
AD  - International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, 
      Academy of Agricultural Sciences, Southwest University, Chongqing, China.
AD  - Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze 
      River (Ministry of Education), Southwest University, Chongqing, China.
FAU - Zhai, Xiao-Di
AU  - Zhai XD
AD  - Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of 
      Plant Protection, Southwest University, Chongqing, China.
AD  - International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, 
      Academy of Agricultural Sciences, Southwest University, Chongqing, China.
AD  - Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze 
      River (Ministry of Education), Southwest University, Chongqing, China.
FAU - Smagghe, Guy
AU  - Smagghe G
AUID- ORCID: 0000-0001-8334-3313
AD  - Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of 
      Plant Protection, Southwest University, Chongqing, China.
AD  - International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, 
      Academy of Agricultural Sciences, Southwest University, Chongqing, China.
AD  - Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent 
      University, Ghent, Belgium.
AD  - Institute of Entomology, Guizhou University, Guiyang, China.
FAU - Jiang, Hongbo
AU  - Jiang H
AD  - Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of 
      Plant Protection, Southwest University, Chongqing, China.
AD  - International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, 
      Academy of Agricultural Sciences, Southwest University, Chongqing, China.
AD  - Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze 
      River (Ministry of Education), Southwest University, Chongqing, China.
FAU - Wang, Jin-Jun
AU  - Wang JJ
AD  - Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of 
      Plant Protection, Southwest University, Chongqing, China.
AD  - International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, 
      Academy of Agricultural Sciences, Southwest University, Chongqing, China.
AD  - Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze 
      River (Ministry of Education), Southwest University, Chongqing, China.
FAU - Wei, Dong
AU  - Wei D
AUID- ORCID: 0000-0002-3625-4358
AD  - Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of 
      Plant Protection, Southwest University, Chongqing, China.
AD  - International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, 
      Academy of Agricultural Sciences, Southwest University, Chongqing, China.
AD  - Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze 
      River (Ministry of Education), Southwest University, Chongqing, China.
LA  - eng
GR  - 32272527/National Natural Science Foundation of China/
GR  - SWU-XJLJ202304/Special Fund for Youth Team of the Southwest University/
GR  - (CARS-26)/the earmarked fund for China Agriculture Research System/
GR  - B18044/111 Project/
PT  - Journal Article
DEP - 20240226
PL  - England
TA  - Pest Manag Sci
JT  - Pest management science
JID - 100898744
SB  - IM
OTO - NOTNLM
OT  - melon fly
OT  - post-transcriptional regulation
OT  - siRNA
OT  - spermatid
OT  - targeting relationship
OT  - testis
EDAT- 2024/02/26 12:42
MHDA- 2024/02/26 12:42
CRDT- 2024/02/26 11:29
PHST- 2024/02/21 00:00 [revised]
PHST- 2023/12/13 00:00 [received]
PHST- 2024/02/22 00:00 [accepted]
PHST- 2024/02/26 12:42 [pubmed]
PHST- 2024/02/26 12:42 [medline]
PHST- 2024/02/26 11:29 [entrez]
AID - 10.1002/ps.8044 [doi]
PST - aheadofprint
SO  - Pest Manag Sci. 2024 Feb 26. doi: 10.1002/ps.8044.